Transcriptional landscape of cotton roots in response to salt stress at single-cell resolution

作     者：Pengtao Li Qiankun Liu Yangyang Wei Chaozhu Xing Zhongping Xu Fang Ding Yuling Liu Quanwei Lu Nan Hu Tao Wang Xiangqian Zhu Shuang Cheng Zhaoguo Li Zilin Zhao Yanfang Li Jiangping Han Xiaoyan Cai Zhongli Zhou Kunbo Wang Baohong Zhang Fang Liu Shuangxia Jin Renhai Peng 

作者机构：College of Biology and Food EngineeringAnyang Institute of TechnologyAnyangHenan 455000China State Key Laboratory of Cotton BiologyInstitute of Cotton ResearchChinese Academy of Agricultural SciencesAnyangHenan 455000China Research BaseAnyang Institute of TechnologyState Key Laboratory of Cotton BiologyAnyang 455000HenanChina School of Life SciencesZhengzhou UniversityZhengzhouHenan 450001China Hubei Hongshan LaboratoryNational Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhanHubeiChina Hubei Key Laboratory of Plant PathologyHuazhong Agricultural UniversityWuhanHubei 430070China Department of BiologyEast Carolina UniversityGreenvilleNC 27858USA 

出 版 物：《Plant Communications》 (植物通讯（英文）)

年 卷 期：2024年第5卷第2期

页      面：46-66页

核心收录：

学科分类：09[农学] 0903[农学-农业资源与环境] 

基　　金：supported by the National Natural Science Foundation of China (31471548,32272179,and 31801404) the Central Plains Science and Technology Innovation Leader Project (214200510029) the Program for Innovative Research Team (in Science and Technology)in University of Henan Province (20IRTSTHN021) the Science and Technology Development Project of Anyang City (2022C01NY001 and 2022C01NY003) the Doctoral and Postdoctoral Research Fund of Anyang Institute of Technology (BSJ2019014 and BHJ2020002) the Key Scientific Research Project of Henan Higher Education Institutions of China (20A210006) the Zhongyuan Scholars Workstation (224400510020) 

主　　题：cotton salt stress scRNA-seq root cell types virus-induced gene silencing 

摘      要：Increasing soil salinization has led to severe reductions in plant yield and quality,and investigating the mo-lecular mechanism of salt stress response is therefore an urgent *** this study,we systematically analyzed the response of cotton roots to salt stress using single-cell transcriptomics technology;56281 high-quality cells were obtained from 5-day-old lateral root tips of Gossypium arboreum under natural growth conditions and different salt *** cell types with an array of novel marker genes were identified and confirmed by in situ RNA hybridization,and pseudotime analysis of some specific cell types revealed their potential differentiation *** changes in cell numbers under salt stress were observed for outer epidermal and inner endodermal cells,which were significantly enriched in response to stress,amide biosynthetic process,glutathione metabolism,and glycolysis/*** of differentially expressed genes identified in multiple comparisons revealed other functional ag-gregations concentrated on plant-type primary cell wall biogenesis,defense response,phenylpropanoid biosynthesis,and metabolic *** candidate differentially expressed genes encoding transcrip-tion factors or associated with plant hormones also responsive to salt stress were identified,and the func-tion of Ga03G2153,annotated as auxin-responsive GH3.6,was confirmed by virus-induced gene *** GaGH3.6-silenced plants showed a severe stress-susceptible phenotype,and physiological and biochemical measurements indicated that they suffered more significant oxidative *** results suggest that GaGH3.6 might participate in cotton salt tolerance by regulating redox *** thus construct a transcriptional atlas of salt-stressed cotton roots at single-cell resolution,enabling us to explore cellular heterogeneity and differentiation trajectories and providing valuable insights into the mo-lecular mechanisms that underlie plan