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Improvement of copper tolerance of Arabidopsis by transgenic expression of an allene oxide cyclase gene,GhA OC1, in upland cotton(Gossypium hirsutum L.)

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The Crop Journal 2015年第4期3卷 343-352页

作者： yuange wang Huaihua Liu Qingguo Xin Institute of Genetics and Developmental Biology Chinese Academy of Sciences Postdoctoral Research Center of Shandong Shengfeng Seeds Co. Ltd. Yantai Academy of Agricultural Sciences

Allene oxide cyclase(AOC, E 5.***.99.6) is an essential enzyme in the jasmonic acid(JA)biosynthetic pathway and mediates a wide range of adaptive responses. In this report, five AOC genes(Gh AOC1–Gh AOC5) were cloned from upland cotton(Gossypium hirsutum L.),sequenced, and characterized. Real-time PCR analysis indicated that the transcripts of Gh AOCs were abundantly expressed in roots and less in fibers, and regulated in cotton plants under methyl jasmonate(Me JA) and Cu Cl2 stresses. To investigate the role of Gh AOC under copper stress, transgenic Arabidopsis plants overexpressing cotton Gh AOC1 under control of the Cauliflower mosaic virus 35S(Ca MV 35S) promoter were generated. Compared to untransformed plants, Gh AOC1-overexpressing Arabidopsis thaliana plants exhibited markedly higher survival rate, shoot fresh weight, shoot dry weight, and photosynthetic efficiency, and reduced cell membrane damage and lipid peroxidation under copper *** study provides the first evidence that Gh AOC1 plays an important role in copper stress tolerance.

关键词： Upland cotton Gh AOC Expression pattern Overexpression Copper tolerance

Genome-wide analysis and identification of cytokinin oxidase/dehydrogenase(CKX) gene family in foxtail millet(Setaria italica)

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The Crop Journal 2014年第4期2卷 244-254页

Cytokinin oxidase/dehydrogenase(CKX; EC.1.***.99.12) regulates cytokinin(CK) level in plants and plays an essential role in CK regulatory processes. CKX proteins are encoded by a small gene family with a varying number of members in different plants. In spite of their physiological importance, systematic analyses of SiCKX genes in foxtail millet have not yet been examined. In this paper, we report the genome wide isolation and characterization of SiCKXs using bioinformatic methods. A total of 11 members of the family were identified in the foxtail millet genome. SiCKX genes were distributed in seven chromosomes(chromosome 1, 3, 4, 5, 6, 7, and 11). The coding sequences of all the SiCKX genes were disrupted by introns, with numbers varying from one to four. These genes expanded in the genome mainly due to segmental duplication events. Multiple alignment and motif display results showed that all SiCKX proteins share FAD- and CK-binding domains. Putative cis-elements involved in Ca2+-response, abiotic stress response, light and circadian rhythm regulation, disease resistance and seed development were present in the promoters of SiCKX genes. Expression data mining suggested that SiCKX genes have diverse expression patterns. Real-time PCR analysis indicated that all 11 SiCKX genes were up-regulated in embryos under 6-BA treatment, and some were NaCl or PEG inducible. Collectively, these results provide molecular insights into CKX research in plants.

关键词： Foxtail millet CKX gene family Phylogenetic analysis Real-time PCR analysis

Responding of zooplankton to environmental factor changes in the Changjiang River estuarine regions in spring-summer from 2016 to 2020

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Journal of Oceanology and Limnology 2024年第2期42卷 544-559页

作者： Jieqing YANG Dongrong ZHANG yuange CHEN Longling OUYANG Yangyang YANG Jin ZHOU Yunrong SHI East China Sea Fisheries Research Institute Chinese Academy of Fishery SciencesKey Laboratory of East China Sea Fishery Resources ExploitationMinistry of Agriculture and Rural AffairsShanghai 200090China Key Laboratory of Engineering Oceanography Second Institute of OceanographyMinistry of Natural ResourcesHangzhou 310012China College of Marine Ecology and Environment Shanghai Ocean UniversityShanghai 201306China

The estuarine areas are under frequent influence from freshwater intrusion and ocean currents,in which zooplankton species are diversified and variable as they are sensitive to physio-chemical variations in ***,understanding the relationships between zooplankton and environmental factors help us know the water *** achieve co-existence with species in similar ecological group or habit,they could inevitably alter themselves to fit the ecology and adjust the function according to the competitive exclusion in ecological ***,information of the co-existence of dominant species in the Changjiang(Yangtze)River estuary(CRE)and adjacent waters remains *** explored the relationships between dominant zooplankton and environmental factors in the study region in spring-summer from 2016 to 2020,involving particularly the composition of dominant species,ecological groups,their relationships with environmental factors,and co-existence of important species,using the non-multidimensional scale analysis(nMDS)method and redundancy *** show that Labidocera euchaeta and Tortanus vermiculus were dominant species in the study *** turnover rate of dominant zooplankton was greater(>50%)in spring while the species number was higher in *** dominant species were estuarine,offshore,and eurytopic based on the adaptation to *** spring,the ecological groups were dominated by estuarine species,while in summer by estuarine and offshore *** addition,the nMDS showed that the dominant species in the same ecological group were more dispersed and not prominently clustered;the dominant species were staggered among different ecological *** temperature,salinity,pH,dissolved oxygen,and chlorophyll a were the main environmental factors on the distribution of the dominant species in spring,while in summer were dissolved oxygen,temperature,salinity,and *** domination of medusae of Nemopsis bachei and Pleurobrachia globosa in zo

关键词： Changjiang River estuary(CRE) zooplankton dominant species ecological group environmental factor co-existence

In-situ fabrication of PtSe2/GaN heterojunction for self-powered deep ultraviolet photodetector with ultrahigh current on/off ratio and detectivity

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Nano Research 2019年第1期12卷 183-189页

作者： Ranran Zhuo Longhui Zeng Huiyu Yuan Di Wu yuange wang Zhifeng Shi Tingting Xu Yongtao Tian Xinjian Li Yuen Hong Tsang School of Physics and Engineering and Key Laboratory of Material PhysicsZhengzhou UniversityZhengzhou 450052China Department of Applied Physics and Materials Research Center The Hong Kong Polytechnic UniversityHung HomKowloonHong KongChina

The research of ultraviolet photodetectors(UV PDs)have been attracting extensive attention,due to their important applications in many *** this study,PtSe2/GaN heterojunction is in-situ fabricated by synthesis of large-area vertically standing two-dimensional(2D)PtSe2 film on n-GaN *** PtSe2/GaN heterojunction device demonstrates excellent photoresponse properties under illumination by deep UV light of 265 nm at zero bias *** analysis reveals that a high responsivity of 193 mA·W^-1,an ultrahigh specific detectivity of 3.8 × 10^14 Jones,linear dynamic range of 155d B and current on/off ratio of^10^8,as well as fast response speeds of 45/102μs were obtained at zero bias ***,this device response quickly to the pulse laser of 266 nm with a rise time of 172 *** high-performanee PtSe2/GaN heteroj u nction UV PD demonstrated in this work is far superior to previously reported results,suggesting that it has great potential for deep UV detection.

关键词： PtSe2 heterojunction deep ultraviolet photodetectors self-powered

Exploring genetic loci for nitrogen use efficiency in wheat

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Exploring genetic loci for nitrogen use efficiency in wheat

Mapping QTLs for low-nitrogen tolerance in wheat

第八届全国小麦基因组学及分子育种大会

作者： Longxi Zhou Xueqiang Zhao Junbo Yang yuange wang Yiping Tong State Key Laboratory for Plant Cell and Chromosome Engineering Institute of Genetics and Developmental SciencesChinese Academy of Sciences State Key Laboratory of Plant Genomics and National Center for Plant Gene Research(Beijing) Institute of Genetics and Developmental BiologyChinese Academy of Sciences

Nitrogen(N) fertilizer is required for high productivity of wheat(Triticum aestivum).N-deficiency can reduce leaf chlorophyll content and grain yields of *** this study,we observed the wheat line 178 A had higher leaf N concentration and chlorophyll content than the wheat line 178 B under bw N conditions during whole life.178 A also showed bnger leaf greenness persistence than 178 B during grain *** results indicate that 178 A has improved N uptake and utilizatbn ability than 178 *** map genetic bci controlling leaf color and greenness persistence duration(GPD),we evaluated 480 recombinant inbred lines(RILs) derived from 178 A and 178 B under bwand high-N conditbns in two growing *** genotyping the RIL populatbn by using 90 K SNP chip,we constructed a genetic map that contained 1127 *** major quantitative trait locus(QTLs) for leaf chlorophyll content at flowering stage under low-N conditions and three QTLs for GPD were *** major locus for leaf chlorophyll content on chromosome 1 A also has pleiotropic effects on *** most significant locus for GPD,qGPD-6 A,bcates in a 1 cM interval,which corresponds to a 1 Mb region on the physical map of chromosome 6 *** regbn confers a NAC transcript factor NAM-A1,a homologous allele of NAM-B1 controlling leaf senescence and grain protein *** DNA and cDNA sequence cbning reveal that NAM-A1 in178 A has a 1-bp substitutbn causing missense variatbn and a 1-bp frame-shift deletbn leading premature stop of coding sequence compared to that of 178 *** variation in CDS probably cause change of transcriptional activation activity of *** downstream genes of NAM-A1 are under *** genes for these major QTLs still need further identificatbn.

关键词： Wheat(Triticum aestivum) Nitrogen use efficiency QTL mapping Chforophyll content Leaf senescence

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Mapping QTLs for low-nitrogen tolerance in wheat

第八届全国小麦基因组学及分子育种大会

作者： Longxi Zhou Xueqiang Zhao Junbo Yang yuange wang Yiping Tong State Key Laboratory for Plant Cell and Chromosome Engineering Institute of Genetics and Developmental SciencesChinese Academy of Sciences State Key Laboratory of Plant Genomics andNational Center for Plant Gene Research(Beijing) Institute of Genetics and Developmental BiologyChinese Academy of Sciences

Nitrogen fertilizer is required for high productivity of wheat(Triticum aestivum).However,excessive fertilizer use is unlikely to be effective at increasing yields and can cause detrimental effects on the environment and human *** key solution to achieve high yield with bwer fertilizer input and reducing environment pollution is to improve nitrogen use efficiency(NUE) in crops.N-defieiency can reduce leaf chlorophyll content and grain yields of *** this study,we observed the wheat line 178 A had higher leafN concentratbn and chbrophyll content than the wheat line 178 B under lowN conditions during whole life,and showed longer leaf greenness persistence than 178 B during grain *** results indicate 178 A is more tolerant to ***480 recombinant inbred lines(RILs) derived from 178 A and 178 B were evaluated under bw-and high-N conditbns in two growing seasons to map genetic locus controlling leaf cobr and greenness persistence duration(GPD).We identified three major QTLs for leaf cobr at flowering stage under bw-N conditbns and three QTLs for *** most significant bcus for leaf cobr on chromosome 1 A also has pleiotropic effects on ***,the major focus for GPD on chromosome 6 A within a 0.49 cM interval explains the highest proportbn of the phenotypie variation According to published wheat genome sequence,this interval focates 30-33 Mb regbn on 6 *** region confers a NAC transcript factor NAM-A1,a homologous allele of NAM-B1 controlling leaf senescence and grain protein concentratbn Genomic DNA and cDNA sequence cfoning reveals that NAM-A1 in 178 A has a 1-bp frame-shift deletion leading premature stop of coding ***,candidate genes for these major QTLs still need to be further configuration.

关键词： Triticum aestivum Low-nitrogen toterance Ggreenness persistence duration QTL NAM-A1

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ABC分类管理法在成品油库存中的应用

广东化工 2008年第7期35卷 54-57页

作者：王远革中石化股份有限公司茂名分公司销售中心广东茂名525011

ABC管理法是根据事物在技术或经济方面的主要特征,进行分类排队,分清重点和一般,从而有区别地确定管理方式的一种分析方法。结合炼油生产特点,针对茂石化成品油存储、外运现状,利用ABC分类管理法对其进行分类监管、重点控制,从而降低企... 详细信息

ABC管理法是根据事物在技术或经济方面的主要特征,进行分类排队,分清重点和一般,从而有区别地确定管理方式的一种分析方法。结合炼油生产特点,针对茂石化成品油存储、外运现状,利用ABC分类管理法对其进行分类监管、重点控制,从而降低企业生产成本,实现效益最大化。

关键词： ABC分类管理法成品油库存效益

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试析WTTC旅游卫星账户的主要特点

北京第二外国语学院学报 2006年第7期28卷 84-88页

作者：王元歌北京第二外国语学院英语教学部北京100024

世界旅游及旅行理事会(WTTC)是全球旅游和旅行业的领袖论坛,在全球旅游和旅行业中表现得非常活跃和突出,它所举行的各种活动也受到了各界认可。本文主要针对WTTC的重要活动———旅游卫星账户的内容和特点进行了简要分析,并认为WTTC旅... 详细信息

世界旅游及旅行理事会(WTTC)是全球旅游和旅行业的领袖论坛,在全球旅游和旅行业中表现得非常活跃和突出,它所举行的各种活动也受到了各界认可。本文主要针对WTTC的重要活动———旅游卫星账户的内容和特点进行了简要分析,并认为WTTC旅游卫星账户的研究方法和主要概念结构值得我们借鉴,而我国也需要尽快建立起旅游卫星账户。

关键词：世界旅游及旅行理事会旅游及旅行业旅游卫星账户

肿瘤科医护人员对化疗所致味觉改变认知的质性研究

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中国实用护理杂志 2024年第17期40卷 1332-1337页

作者：董元鸽杨瑒张晓菊陆箴琦复旦大学附属肿瘤医院护理部、复旦大学上海医学院肿瘤学系上海200032

目的深入了解肿瘤科医护人员对化疗所致味觉改变(CITAs)方面的认知。方法采取描述性质性研究的方法,于2023年8—11月采用目的抽样法对复旦大学附属肿瘤医院15名肿瘤内科医护人员进行深入访谈,采用Colaizzi 7步分析法对访谈资料进行转录... 详细信息

目的深入了解肿瘤科医护人员对化疗所致味觉改变(CITAs)方面的认知。方法采取描述性质性研究的方法,于2023年8—11月采用目的抽样法对复旦大学附属肿瘤医院15名肿瘤内科医护人员进行深入访谈,采用Colaizzi 7步分析法对访谈资料进行转录、编码、类属分析及描述。结果15名受访者年龄为26~43岁,男3名,女12名。共提炼出3个主题,CITAs症状管理的益处感知("硬结局"之益处感知-提高症状管理水平;"软结局"之益处感知-增进多方有效沟通);CITAs相关知识的认知匮乏阻碍了有效的症状管理(医护人员对CITAs相关知识的认知存在差异;CITAs相关知识获取渠道的可及性较低);CITAs的管理与干预措施缺乏专业化与高效率(CITAs的管理措施多源于工作经验;未来CITAs的管理可能基于信息化建设)。结论管理者应充分利用CITAs症状管理契机,制订专业化且高效的干预及管理策略,加强肿瘤科医护人员对于该症状相关知识的学习,减少症状困扰及患者自我感受负担,以提高肿瘤内科医护人员症状管理的水平。

关键词：定性研究味觉改变认知