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Genome-wide analysis and identification of cytokinin oxidase/dehydrogenase(CKX) gene family in foxtail millet(Setaria italica)

Genome-wide analysis and identification of cytokinin oxidase/dehydrogenase(CKX) gene family in foxtail millet(Setaria italica)

作     者:Yuange Wang Huaihua Liu Qingguo Xin 

作者机构:Institute of Genetics and Developmental Biology Chinese Academy of Sciences Postdoctoral Research Center of Shandong Shengfeng Seeds Co. Ltd. Yantai Academy of Agricultural Sciences 

出 版 物:《The Crop Journal》 (作物学报(英文版))

年 卷 期:2014年第2卷第4期

页      面:244-254页

学科分类:09[农学] 0901[农学-作物学] 

基  金:supported by the project of the Modern Seed Industry Enterprise Science and Technology Development of Shandong Province  China (SDKJ2012QF003) 

主  题:Foxtail millet CKX gene family Phylogenetic analysis Real-time PCR analysis 

摘      要:Cytokinin oxidase/dehydrogenase(CKX; EC.1.***.99.12) regulates cytokinin(CK) level in plants and plays an essential role in CK regulatory processes. CKX proteins are encoded by a small gene family with a varying number of members in different plants. In spite of their physiological importance, systematic analyses of SiCKX genes in foxtail millet have not yet been examined. In this paper, we report the genome wide isolation and characterization of SiCKXs using bioinformatic methods. A total of 11 members of the family were identified in the foxtail millet genome. SiCKX genes were distributed in seven chromosomes(chromosome 1, 3, 4, 5, 6, 7, and 11). The coding sequences of all the SiCKX genes were disrupted by introns, with numbers varying from one to four. These genes expanded in the genome mainly due to segmental duplication events. Multiple alignment and motif display results showed that all SiCKX proteins share FAD- and CK-binding domains. Putative cis-elements involved in Ca2+-response, abiotic stress response, light and circadian rhythm regulation, disease resistance and seed development were present in the promoters of SiCKX genes. Expression data mining suggested that SiCKX genes have diverse expression patterns. Real-time PCR analysis indicated that all 11 SiCKX genes were up-regulated in embryos under 6-BA treatment, and some were NaCl or PEG inducible. Collectively, these results provide molecular insights into CKX research in plants.

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