Improvement of copper tolerance of Arabidopsis by transgenic expression of an allene oxide cyclase gene,GhA OC1, in upland cotton(Gossypium hirsutum L.)
Improvement of copper tolerance of Arabidopsis by transgenic expression of an allene oxide cyclase gene,GhA OC1, in upland cotton(Gossypium hirsutum L.)作者机构:Institute of Genetics and Developmental Biology Chinese Academy of Sciences Postdoctoral Research Center of Shandong Shengfeng Seeds Co. Ltd. Yantai Academy of Agricultural Sciences
出 版 物:《The Crop Journal》 (作物学报(英文版))
年 卷 期:2015年第3卷第4期
页 面:343-352页
核心收录:
学科分类:0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种]
基 金:supported by the project of Modern Seed Industry Enterprise Science and Technology Development of Shandong Province China (SDKJ2012QF003)
主 题:Upland cotton Gh AOC Expression pattern Overexpression Copper tolerance
摘 要:Allene oxide cyclase(AOC, E 5.***.99.6) is an essential enzyme in the jasmonic acid(JA)biosynthetic pathway and mediates a wide range of adaptive responses. In this report, five AOC genes(Gh AOC1–Gh AOC5) were cloned from upland cotton(Gossypium hirsutum L.),sequenced, and characterized. Real-time PCR analysis indicated that the transcripts of Gh AOCs were abundantly expressed in roots and less in fibers, and regulated in cotton plants under methyl jasmonate(Me JA) and Cu Cl2 stresses. To investigate the role of Gh AOC under copper stress, transgenic Arabidopsis plants overexpressing cotton Gh AOC1 under control of the Cauliflower mosaic virus 35S(Ca MV 35S) promoter were generated. Compared to untransformed plants, Gh AOC1-overexpressing Arabidopsis thaliana plants exhibited markedly higher survival rate, shoot fresh weight, shoot dry weight, and photosynthetic efficiency, and reduced cell membrane damage and lipid peroxidation under copper *** study provides the first evidence that Gh AOC1 plays an important role in copper stress tolerance.