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Piezo1 channel activation in response to mechanobiological acoustic radiation force in osteoblastic cells

Bone Research 2021年第2期9卷 223-232页

作者： Guangdao Zhang Xiaofei Li Lin Wu yi-xian qin Department of Biomedical Engineering Stony Brook UniversityStony BrookNY 11794USA Department of Prosthodontics School of StomatologyChina Medical UniversityShenyangChina

Mechanobiological stimuli,such as low-intensity pulsed ultrasound(LIPUS),have been shown to promote bone regeneration and fresh fracture repair,but the fundamental biophysical mechanisms involved remain ***,we propose that a mechanosensitive ion channel of Piezo1 plays a pivotal role in the noninvasive ultrasound-induced mechanical transduction pathway to trigger downstream cellular signal *** study aims to investigate the expression and role of Piezo1 in MC3T3-E1 cells after LIPUS *** analysis shows that Piezo1 was present on MC3T3-E1 cells and could be ablated by shRNA ***3T3-E1 cell migration and proliferation were significantly increased by LIPUS stimulation,and knockdown of Piezo1 restricted the increase in cell migration and *** labeling with Fluo-8,MC3T3-E1 cells exhibited fluorescence intensity traces with several high peaks compared with the baseline during LIPUS *** obvious change in the fluorescence intensity tendency was observed after LIPUS stimulation in shRNA-Piezo1 cells,which was similar to the results in the GsMTx4-treated *** phosphorylation ratio of ERK1/2 in MC3T3-E1 cells was significantly increased(P<0.01)after LIPUS *** addition,Phalloidin-iFluor-labeled F-actin filaments immediately accumulated in the perinuclear region after LIPUS stimulation,continued for 5 min,and then returned to their initial levels at 30 *** results suggest that Piezo1 can transduce LIPUS-induced mechanical signals into intracellular *** influx of Ca2+serves as a second messenger to activate ERK1/2 phosphorylation and perinuclear F-actin filament polymerization,which regulate the proliferation of MC3T3-E1 cells.

关键词： stimulation Piezo1 activation

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Calcium phosphate coatings enhance biocompatibility and degradation resistance of magnesium alloy:Correlating in vitro and in vivo studies

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Bioactive Materials 2021年第5期6卷 1223-1229页

作者： Julia Gao yingchao Su yi-xian qin Department of Biomedical Engineering Stony Brook UniversityStony BrookNY11794United States

Magnesium(Mg)and its alloys are promising biodegradable materials for orthopedic ***,one of the major problems is their rapid degradation rate with quick evolution of hydrogen *** overcome this problem,calcium phosphate(CaP)coatings have been used to improve the degradation resistance and the biocompatibility of Mg *** study focuses on the comparison and correlation of the in vitro and in vivo degradation and biocompatibility behaviors of these materials.A CaP coating consisting of dicalcium phosphate dihydrate(DCPD)was deposited on an AZ60 Mg alloy by the chemical conversion ***,the in vitro degradation testing including electrochemical and immersion tests,and in vivo implantation of the CaP coated Mg alloy were conducted to compare the degradation ***,the in vitro cell behavior and in vivo bone tissue response were also compared on both uncoated and CaP-coated Mg *** showed that the CaP coating provided the Mg alloy with significantly better biodegradation behavior and *** in vitro and in vivo biocompatibility tests exhibited good consistency while not the case for *** showed that the in vitro electrochemical test could be a quick screening tool for the biodegradation rate,while the in vitro immersion degradation rate was often 2-4 folds faster than the in vivo degradation rate.

关键词： Magnesium implants Calcium phosphate Biodegradation Cytocompatibility Bone regeneration

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Bioactive superparamagnetic iron oxide-gold nanoparticles regulated by a dynamic magnetic field induce neuronal Ca^(2+)influx and differentiation

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Bioactive Materials 2023年第8期26卷 478-489页

作者： Elias Georgas Muzhaozi Yuan Jingfan Chen Ya Wang yi-xian qin Department of Biomedical Engineering The State University of New York at Stony BrookStony BrookNYUnited States J.Mike Walker’66 Department of Mechanical Engineering Texas A&M UniversityCollege StationTXUnited States Department of Biomedical Engineering Texas A&M UniversityCollege StationTXUnited States Department of Electrical and Computer Engineering Texas A&M UniversityCollege StationTXUnited States

Treating neurodegenerative diseases,e.g.,Alzheimer’s Disease,remains a significant challenge due to the limited neuroregeneration rate in the *** objective of this study is to evaluate the hypothesis that external magnetic field(MF)stimulation of nerve growth factor functionalized superparamagnetic iron oxide-gold(NGF-SPIO-Au)nanoparticles(NPs)can induce Ca^(2+)influx,membrane depolarization,and enhance neuron differentiation with dynamic MF(DMF)outperforming static MF(SMF)*** showed the that total intracellular Ca^(2+)influx of PC-12 cells was improved by 300%and 535%by the stimulation of DMF(1 Hz,0.5 T,30min)with NGF-SPIO-Au NPs compared to DMF alone and SMF with NGF-SPIO-Au NPs,respectively,which was attributed to successive membrane *** uptake performed with the application of sodium azide proved that DMF enhanced cellular uptake of NGF-SPIO-Au NPs via *** addition,DMF upregulated both the neural differentiation marker(β3-tubulin)and the cell adhesive molecule(integrin-β1)with the existence of NGF-SPIO-Au NPs,while SMF did not show these *** results imply that noninvasive DMF-stimulated NPs can regulate intracellular Ca^(2+)influx and enhance neuron differentiation and neuroregeneration rate.

关键词： Calcium influx Membrane potential Dynamic magnetic fields Neuroregeneration SPIO-Au Core-shell nanoparticles

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Dynamic Fluid Flow Mechanical Stimulation Modulates Bone Marrow Mesenchymal Stem Cells

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Bone Research 2013年第1期1卷 98-104页

作者： Minyi Hu RobbinYeh Michelle Lien Morgan Teeratananon KunalAgarwal yi-xian qin Department of Biomedical Engineering Stony Brook UniversityStony BrookNY 11794-5281USA

Osteoblasts are derived from mesenchymal stem cells （MSCs）, which initiate and regulate bone formation. New strategies for osteoporosis treatments have aimed to control the fate of MSCs. While functional disuse decreases MSC growth and osteogenic potentials, mechanical signals enhance MSC quantity and bias their differentiation toward osteoblastogenesis. Through a non-invasive dynamic hydraulic stimulation （DHS）, we have found that DHS can mitigate trabecular bone loss in a functional disuse model via rat hindlimb suspension （HLS）. To further elucidate the downstream cellular effect of DHS and its potential mechanism underlying the bone quality enhancement, a longitudinal in vivo study was designed to evaluate the MSC populations in response to DHS over 3, 7, 14, and 21 days. Five-month old female Sprague Dawley rats were divided into three groups for each time point： age-matched control, HLS, and HLS＋DHS. DHS was delivered to the right mid-tibiae with a daily ＂10 min on-5 min off-10 min on＂ loading regime for five days/week. At each sacrifice time point, bone marrow MSCs of the stimulated and control tibiae were isolated through specific cell surface markers and quantified by flow cytometry analysis. A strong time-dependent manner of bone marrow MSC induction was observed in response to DHS, which peaked on day 14. After 21 days, this effect of DHS was diminished. This study indicates that the MSC pool is positively influenced by the mechanical signals driven by DHS. Coinciding with our previous findings of mitigation of disuse bone loss, DHS induced changes in MSC number may bias the differentiation of the MSC population towards osteoblastogenesis, thereby promoting bone formation under disuse conditions. This study provides insights into the mechanism of time-sensitive MSC induction in response to mechanical loading, and for the optimal design of osteovorosis treatments.

关键词： bone adaptation mechanical loading noninvasive stimulation osteoporosis osteopenia

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Biofunctionalization of metallic implants by calcium phosphate coatings

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Bioactive Materials 2019年第1期4卷 196-206页

作者： yingchao Su Irsalan Cockerill Yufeng Zheng Liping Tang yi-xian qin Donghui Zhu Department of Biomedical Engineering University of North TexasDentonTXUSA Department of Materials Science and Engineering College of EngineeringPeking UniversityBeijingChina Department of Bioengineering University of Texas at ArlingtonArlingtonTXUSA Department of Biomedical Engineering Stony Brook UniversityStony BrookNYUSA

Metallic materials have been extensively applied in clinical practice due to their unique mechanical properties and *** years have witnessed broad interests and advances on surface functionalization of metallic implants for high-performance *** phosphates(CaPs)are the major inorganic component of bone tissues,and thus owning inherent biocompatibility and osseointegration *** such,they have been widely used in clinical orthopedics and *** new emergence of surface functionalization on metallic implants with CaP coatings shows promise for a combination of mechanical properties from metals and various biofunctions from *** review provides a brief summary of state-of-art of surface biofunctionalization on implantable metals by CaP *** first glance over different types of CaPs with their coating methods and in vitro and in vivo performances,and then give insight into the representative biofunctions,***,corrosion resistance and biodegradation control,and antibacterial property,provided by CaP coatings for metallic implant materials.

关键词： Calcium phosphates Metallic implant materials Surface biofunctionalization Osteointegration Biodegradation

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Design and simulation of a 2-D array flexible ultrasound transducer system for tissue characterization-A pilot study

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Medicine in Novel Technology and Devices 2022年第1期13卷 59-64页

作者： Zhihua Gan Brian Guo Jiqi Cheng yi-xian qin Department of Biomedical Engineering Stony Brook UniversityStony BrookNY11794USA Department of Electrical and Computer Engineering Stony Brook UniversityStony BrookNY11794USA

Noninvasive diagnosis of bone density and mechanical properties using non-radiation imaging modality is an emerging area with promising in early prediction of osteopenia and treatment effectiveness in the clinic and functional disuse,i.e.,long-term bedrest and space *** in quantitative ultrasound have shown advantages in measuring both bone density and mechanical strength,non-radiation,imaging capability,and easy to *** challenge that remained is the poor penetration of ultrasound signals passing through trabecular and cortical bones and acoustic energy scattering.A new scanning confocal ultrasound technology is developed in this lab to detect the alteration of bone to provide diagnostic results in bone density and structure properties.A software-controlled flexible ultrasound system with 2-D dual array transducer is developed and proposed for the purpose of noninvasive bone density diagnosis and assessment of bone ***(Tx)transducer elements are divided into sub-blocks to excite the ultrasound signals in sequence to decrease the system complexity while maintaining beam pattern properties by the signal processing procedure at receiving(Rx)*** is also applied to reduce acoustic side lobes and to make the resolution in the ultrasound field of view(FOV)more *** study may provide basic understanding of modulated confocal ultrasound beam forming for tissue characterization,such as trabecular bone structual and strength properties.

关键词： Flexible ultrasound system Noninvasive diagnostic imaging Sub-block triggering Ultrasound imaging resolution Apodization Quantitative ultrasound Trabecular bone property Scanning ultrasound Bone density and strength

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Blending with transition metals improves bioresorbable zinc as better medical implants

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Bioactive Materials 2023年第2期20卷 243-258页

作者： yingchao Su Jiayin Fu Juncen Zhou Elias Georgas Shaokang Du yi-xian qin Yadong Wang Yufeng Zheng Donghui Zhu Department of Biomedical Engineering Stony Brook UniversityStony BrookNYUSA Nancy E.and Peter C.Meinig School of Biomedical Engineering Cornell UniversityIthacaNYUSA Department of Cardiology Sir Run Run Shaw HospitalZhejiang UniversityHangzhouZhejiangChina Academy for Advanced Interdisciplinary Studies Peking UniversityBeijingChina School of Materials Science and Engineering Peking UniversityBeijingChina

Zinc(Zn)is a new class of bioresorbable metal that has potential for cardiovascular stent material,orthopedic implants,wound closure devices,***,pure Zn is not ideal for these applications due to its low mechanical strength and localized degradation *** is the most common/effective way to overcome this ***,the choice of alloying element is crucial to ensure the resulting alloy possesses sufficient mechanical strength,suitable degradation rate,and acceptable ***,we proposed to blend selective transition metals(i.e.,vanadium-V,chromium-Cr,and zirconium-Zr)to improve Zn’s *** selected transition metals have similar properties to Zn and thus are beneficial for the metallurgy process and mechanical ***,the biosafety of these elements is of less concern as they all have been used as regulatory approved medical implants or a component of an implant such as Ti6Al4V,CoCr,or Zr-based dental *** study showed the first evidence that blending with transition metals V,Cr,or Zr can improve Zn’s properties as bioresorbable medical *** addition,three in vivo implantation models were explored in rats:subcutaneous,aorta,and femoral implantations,to target the potential clinical applications of bioresorbable Zn implants.

关键词： Degradation Cardiovascular Orthopedic Subcutaneous Antibacterial

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Improved mechanical, degradation, and biological performances of Zn–Fe alloys as bioresorbable implants

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Bioactive Materials 2022年第11期7卷 334-343页

作者： yingchao Su Jiayin Fu Wonsae Lee Shaokang Du yi-xian qin Yufeng Zheng Yadong Wang Donghui Zhu Department of Biomedical Engineering Stony Brook UniversityStony BrookNYUSA Nancy E.and Peter C.Meinig School of Biomedical Engineering Cornell UniversityIthacaNYUSA Department of Cardiology Sir Run Run Shaw HospitalZhejiang UniversityHangzhouZhejiangChina Academy for Advanced Interdisciplinary Studies Peking UniversityBeijingChina School of Materials Science and Engineering Peking UniversityBeijingChina

Zinc(Zn)is a promising bioresorbable implant material with more moderate degradation rate compared to magnesium(Mg)and iron(Fe).However,the low mechanical strength and localized degradation behavior of pure Zn limit its clinical *** is one of the most effective ways to overcome these *** screening the alloying element candidates regarding their potentials for improvement on the degradation and biocompatibility,we proposed Fe as the alloying element for Zn,and investigated the in vitro and in vivo performances of these alloys in both subcutaneous and femoral *** showed that the uniformly distributed secondary phase in Zn–Fe alloys significantly improved the mechanical property and facilitated uniform degradation,which thus enhanced their biocompatibility,especially the Zn-0.4Fe ***,these Zn–Fe alloys showed outstanding antibacterial *** together,Zn–Fe alloys could be promising can-didates as bioresorbable medical implants for various cardiovascular,wound closure,and orthopedic applications.

关键词： Zinc Mechanical Degradation Biocompatibility Antibacterial

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Mechanobiology in cellular,molecular,and tissue adaptation

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Mechanobiology in Medicine 2023年第2期1卷 1-7页

作者： yi-xian qin Jie Zhao Stony Brook University Stony BrookNYUSA Ninth People’s Hospital Shanghai Jiao Tong UniversityShanghaiChina

The use of mechanical biology and biomechanical signal transduction is a novel approach to attenuate biological tissue degeneration,whereas the understanding of specific cellular responses is critical to delineate the underlying *** mechanical signals with optimized loading signals,i.e.,intensity and frequency,have been shown to have the potential to regulate adaptation and *** pathways are of great interest in elucidating how mechanical signals produce such observed effects,including reduced tissue mass loss,increased healing and formation,and cell *** mechanobiology in the adaptation of cells and tissues is observed and recorded in the literature,its application in disease mechanism and treatment is under *** would congratulate the opening of the Mechanobiology in Medicine journal,which provides an effective platform for advanced research in basic mechanotransduction and its translation in disease diagnosis,therapeutics,and *** review aims to develop a cellular and molecular understanding of the mecha-notransduction processes in tissue regeneration,which may provide new insights into disease mechanisms and the promotion of *** attention is allotted to the responses of mechanical loading,including potential cellular and molecular pathways,such as mechanotransduction associated with mechanotransduction pathways(e.g.,Piezo ion channels and Wnt signaling),immune-response,neuron development,tissue adaptation and repair,and stem cell ***,these discussed data highlight the complex yet highly coordi-nated mechanotransduction process in tissue regeneration.

关键词： Biomechanics&mechanobiology Bone adaptation Osteopenia Cardiovascularadaptation Piezo 1 Muscle atrophy Loadingfrequency Ultrasoundtreatment Cartilageregeneration

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Electrocatalytic cleavage of a lignin β-O-4 model compound and coupling with nitrogen-containing aromatics using Prussian blue analogue-derived nickel–cobalt spinel

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Journal of Energy Chemistry 2025年第1期100卷 628-636页

作者： yi Qi Xueying Chen Bowen Liu Xuliang Lin Xueqing Qiu Yanlin qin Guangdong Provincial Key Laboratory of Plant Resources Biorefinery School of Chemical Engineering and Light Industry Guangdong University of Technology Guangdong Provincial Laboratory of Chemistry and Fine Chemical Engineering Jieyang Center Guangdong Basic Research Center of Excellence for Ecological Security and Green Development Guangdong University of Technology

Electrochemical conversion of lignin for the production of high-value heterocyclic aromatic compounds has great *** demonstrate the targeted synthesis and cation modulation of NiCo2O4spinel nanoboxes,synthesized via cation exchange and calcination *** catalysts exhibit excellent efficacy in the electrocatalytic conversion of lignin model compounds,specifically 2-phenoxy-1-phenylethanol,into nitrogen-containing aromatics,achieving high conversion rates and *** catalysts were synthesized via a cation exchange and calcination oxidation process,using Prussian blue nanocubes as *** porous architecture and polymetallic composition of the NiCo2O4spinel demonstrated superior performance in electrocatalytic oxidative coupling,achieving a 99.2 wt%conversion rate of the 2-phenoxy-1-phenylethanol with selectivities of 37.5 wt%for quinoline derivatives and 31.5 wt%for *** innovations include the development of a sustainable one-pot synthesis method for quinoline derivatives,the elucidation of a multistage reaction pathway involving CAO bond cleavage,hydroxyaldol condensation,and CAN bond formation,and a deeper mechanistic understanding derived from DFT *** work establishes a new strategy for lignin valorization,offering a sustainable route to produce high-value nitrogen-containing aromatics from renewable biomass under mild conditions,without the need for additional reagents.

关键词： Spinel Biomass valorization Electrocatalysis Lignin oxidation Coupling

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