Piezo1 channel activation in response to mechanobiological acoustic radiation force in osteoblastic cells

作     者：Guangdao Zhang Xiaofei Li Lin Wu Yi-Xian Qin Guangdao Zhang;Xiaofei Li;Lin Wu;Yi-Xian Qin

作者机构：Department of Biomedical EngineeringStony Brook UniversityStony BrookNY 11794USA Department of ProsthodonticsSchool of StomatologyChina Medical UniversityShenyangChina 

出 版 物：《Bone Research》 (骨研究（英文版）)

年 卷 期：2021年第9卷第2期

页      面：223-232页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：supported by the National Institute of Health(R01AR052379 and R01AR61821,YXQ) GZ is partially supported by a fellowship from the Dental School of the Chinese Medical University during his studies at Stony Brook University 

主　　题：stimulation Piezo1 activation 

摘      要：Mechanobiological stimuli,such as low-intensity pulsed ultrasound(LIPUS),have been shown to promote bone regeneration and fresh fracture repair,but the fundamental biophysical mechanisms involved remain ***,we propose that a mechanosensitive ion channel of Piezo1 plays a pivotal role in the noninvasive ultrasound-induced mechanical transduction pathway to trigger downstream cellular signal *** study aims to investigate the expression and role of Piezo1 in MC3T3-E1 cells after LIPUS *** analysis shows that Piezo1 was present on MC3T3-E1 cells and could be ablated by shRNA ***3T3-E1 cell migration and proliferation were significantly increased by LIPUS stimulation,and knockdown of Piezo1 restricted the increase in cell migration and *** labeling with Fluo-8,MC3T3-E1 cells exhibited fluorescence intensity traces with several high peaks compared with the baseline during LIPUS *** obvious change in the fluorescence intensity tendency was observed after LIPUS stimulation in shRNA-Piezo1 cells,which was similar to the results in the GsMTx4-treated *** phosphorylation ratio of ERK1/2 in MC3T3-E1 cells was significantly increased(P0.01)after LIPUS *** addition,Phalloidin-iFluor-labeled F-actin filaments immediately accumulated in the perinuclear region after LIPUS stimulation,continued for 5 min,and then returned to their initial levels at 30 *** results suggest that Piezo1 can transduce LIPUS-induced mechanical signals into intracellular *** influx of Ca2+serves as a second messenger to activate ERK1/2 phosphorylation and perinuclear F-actin filament polymerization,which regulate the proliferation of MC3T3-E1 cells.