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Effects of etanercept on the apoptosis of ganglion cells and expression of Fas, TNF-α, caspase-8 in the retina of diabetic rats

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International Journal of Ophthalmology(English edition) 2019年第7期12卷 1083-1088页

作者： Qin ye Yu-Ni Lin Mao-Song Xie Yi-Hua Yao Shu-Min Tang Yan Huang Xiao-Hui Wang Yi-Hua Zhu Department of Ophthalmology the First Affiliated Hospital of Fujian Medical University Fuzhou 350005Fujian ProvinceChina XiaMen Haicang Hospital Xiamen 361026Fujian ProvinceChina Fijian Medical University Fuzhou 350005Fujian ProvinceChina

AIM: To evaluate the effects of etanercept on the expression of Fas, tumor necrosis factor-alpha(TNF-α) and caspase-8 in the early stage of the apoptotic pathway in diabetic rats, and to explore the therapeutic effect of etanercept on diabetic ***: A total of 60 Sprague-Dawley(SD) rats were randomly and evenly divided into 3 groups with 20 rats each, including control group, and diabetic groups with or without treatment. Streptozotocin(STZ)-induced diabetic rats were established for diabetic groups. Blood glucose and body weight were measured weekly. All the rats were sacrificed at the 12 wk after treatment. The expressions of Fas, TNF-α and caspase-8 in rat retina were quantitatively detected by pCR and Western blot. The leakage of Evan blue was adopted to measure the retinal vascular leakage quantitatively, and to compare it among different groups. TUNEL method was used to compare the amount of apoptotic bodies quantitatively in rat retina ganglion cells under electron ***: The expressions of Fas, TNF-α and caspase-8 in each group were compared via pCR and Western blot, in which the diabetic group with treatment was lower than those without treatment(pps were higher than the control group(pp was lower than those without treatment(pp was the lowest compared with the other two groups(ppoptoticbodies of retina in the diabetic treatment group was lower than those without treatment(pp was the lowest compared with the other two groups(ppt can effectively reduce the expression of Fas, TNF-α and caspase-8, as well as the retinal leakage and retinal cell apoptosis in diabetic rats.

关键词： etanercept ganglion cells Fas tumor necrosis factor-alpha caspase-8 apoptosis diabetes rat

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单侧双通道内镜治疗重度腰椎管狭窄症的近期疗效分析

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浙江医学 2024年第12期46卷 1300-1304页

作者：占允中杨帆叶舟赵梓汝刘华飞吴以臣黄伟铖衢州市人民医院(温州医科大学附属衢州医院)脊柱外科 324000 衢州市人民医院(温州医科大学附属衢州医院)高压氧科 324000

目的探讨单侧双通道内镜治疗重度腰椎管狭窄症的近期疗效及对患者硬膜囊面积、功能恢复情况的影响。方法前瞻性选取2021年1月至2023年1月衢州市人民医院收治的重度腰椎管狭窄症患者60例为研究对象,采用随机数字表法分为观察组和对照组,... 详细信息

目的探讨单侧双通道内镜治疗重度腰椎管狭窄症的近期疗效及对患者硬膜囊面积、功能恢复情况的影响。方法前瞻性选取2021年1月至2023年1月衢州市人民医院收治的重度腰椎管狭窄症患者60例为研究对象,采用随机数字表法分为观察组和对照组,各30例。观察组实施单侧双通道内镜技术,对照组实施传统腰椎后路椎板开窗减压术,比较两组患者手术效果、硬膜囊面积变化、功能恢复及并发症发生情况。结果观察组患者总优良率为93.33%,高于对照组的73.33%,差异有统计学意义(pppp>0.05)。结论单侧双通道内镜治疗重度腰椎管狭窄症近期疗效显著,可明显减轻患者术后疼痛程度,改善患者腰椎功能。

关键词：重度腰椎管狭窄症单侧双通道内镜治疗近期疗效硬膜囊面积功能恢复情况

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下位协调依赖:对属地管理扩张的一个解释框架——以沪郊p街道的经验为例

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公共管理学报 2022年第1期19卷 84-94,171页

作者：叶敏华东理工大学社会与公共管理学院上海200237

属地管理向基层扩张已经成为加重基层负担的渊薮,而属地管理为何向基层扩张则存在理论解释的盲点。本文试图将属地管理向基层扩张现象理解为中国治理实践中的"模糊治理体系"对基层下位协调的功能性依赖。"模糊治理体系"的有效治理高度... 详细信息

属地管理向基层扩张已经成为加重基层负担的渊薮,而属地管理为何向基层扩张则存在理论解释的盲点。本文试图将属地管理向基层扩张现象理解为中国治理实践中的"模糊治理体系"对基层下位协调的功能性依赖。"模糊治理体系"的有效治理高度依赖两种协调机制的串联,即自上而下的上位统合机制和以基层属地管理为基础的自下而上的下位协调机制。在社会治理精细化和目标多元化的治理情境下,上位统合机制越来越难以承担治理体系内部职责关系协调和政社关系协调任务,由此导致治理系统对基层属地管理下位协调机制的高度依赖。基层属地管理的下位协调机制虽然有着"以模糊治理模糊"的功能,提升了治理任务的完成度,但也造成了基层"超载"的治理内卷化问题。

关键词：属地管理基层下位协调治理

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Molecular mechanism underlying the subtype-selectivity of competitive inhibitor NF110 and its distinct potencies in human and rat p2X3 receptors

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Science Bulletin 2018年第24期63卷 1616-1625页

作者： Bin Li Jin Wang Xiaoyang Cheng Yan Liu Yang Yang Xiaona Yang Changrun Guod Youya Niu peng Cao Xiangyang Lu Michael X.Zhu Yun Tian ye Yu College of Biosdence and Biotechnology.Hunan Agricultural University Changsha 410128China State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources School of Chemistry and Pharmaceutical ScienceGuangxi Normal UniversityGuilin 541004China Department of pharmacology and Chemical Biology Institute of Medical SciencesShanghai Jiao Tong University School of MedicineShanghai 200025China School of Basic Medicine and Clinical pharmacy China Pharmaceutical UniversityNanjing 210009China Department of Cell Biology and Genetics Hunan University of MedicineHuaihua 418000China Hospital of Integrated Traditional Chinese and Western Medicine Nanjing University of Chinese MedicineNanjing 210023China

p2X receptors are a family of extracellular ATp-gated trimeric cation channels that is widely distributed in human tissues. Quite some drug candidates targeting p2X receptors have entered into preclinical or main phases of clinical trials, but many of them failed due to low subtype-selectivity or species differences in pharmacological activities between human and experimental animals. Here, we identified the distinct inhibitory efficacies of NF110, a competitive inhibitor, between the rat(rp2X3) and human(hp2X3) p2X3 receptors. We demonstrated that this difference is determined by two amino acids located in the dorsal fin(DF) domain of p2X3 receptors. As revealed by mutagenesis, metadynamics, and covalent modification, NF110-mediated rp2X3 inhibition may be through a filling in the cavity formed by the DF,left flipper(LF) and lower body(LB) to partially, rather than fully, occupy the ATp-binding ***, substitution of residues located in the DF and/or LF domains of the rp2X2 receptor, a NF110-insensitive subtype, with the equivalent amino acids of rp2X3, bestowed the sensitivity of rp2X2 to NF110. The critical roles of the DF and LF domains in channel gating of p2X and low conservativity in residue sequences of those two domains raise the possibility that small molecules differentially interacting with the residues of the DF and LF domains of different p2X receptors may modulate channel's activity in a subtype-selective manner. However, the possible species-specificity of p2X inhibitors/modulators makes it more complex when interpreting the preclinical data into clinical ***, our data provide new insights into the subtype-selectivity of competitive inhibitors and their distinct potencies in the human and experimental animals, both of which are extremely important in the drug discovery of p2X receptors.

关键词： p2X receptors Competitive inhibitors NF110 Species-specificity Subtype-selectivity

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血清乙型肝炎表面抗原及乙型肝炎病毒RNA水平在预测核苷(酸)类药物治疗慢性乙型肝炎患者停药后复发中的价值

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中国当代医药 2024年第28期31卷 127-130页

作者：何小燕张丽琴吴天恩曾瑞丽叶文峰黎翠兰江西省赣州市人民医院检验科江西赣州341000

目的分析乙型肝炎表面抗原(HbsAg)及乙型肝炎病毒RNA(HBV RNA)水平检测对核苷(酸)类药物(NAs)治疗慢性乙型肝炎(CHB)患者停药后复发的预测效果。方法选取2020年1月至2021年1月在赣州市人民医院使用NAs治疗并达到停药标准的80例CHB患者,... 详细信息

目的分析乙型肝炎表面抗原(HbsAg)及乙型肝炎病毒RNA(HBV RNA)水平检测对核苷(酸)类药物(NAs)治疗慢性乙型肝炎(CHB)患者停药后复发的预测效果。方法选取2020年1月至2021年1月在赣州市人民医院使用NAs治疗并达到停药标准的80例CHB患者,展开1年随访,根据有无病毒学复发分为复发组和未复发组。采集两组患者血液标本,展开HBV RNA及HBsAg定量检测,分析HBsAg和HBV RNA定量对NAs药物停药后复发的预测价值。结果80例患者复发率17.50%,复发组的停药时HBV DNA载量、停药时HBsAg定量高于未复发组,差异有统计学意义(pp<0.05)。HBV RNA预测疾病复发的敏感度为85.71%,特异度80.30%,HBsAg对应数值为78.57%、89.39%。结论HBsAg和HBV RNA定量对NAs药物停药后CHB复发有预测价值,值得推广。

关键词：慢性乙型肝炎核苷(酸)类药物停药复发乙型肝炎表面抗原乙型肝炎病毒RNA 预测效能

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不同强度有氧跑有助于改善IGR老年人的症状

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基因组学与应用生物学 2019年第7期38卷 3383-3389页

作者：叶琼重庆幼儿师范高等专科学校

为了探讨不同强度持续跑步干预对糖调节受损(impaired glucose regulation,IGR)人群胰岛素敏感性、骨密度、糖调节、激素分泌的影响效果差异,为研发促进IGR人群糖调节能力恢复的运动处方提供实证参考,本研究选定90名糖调节受损老年人为... 详细信息

为了探讨不同强度持续跑步干预对糖调节受损(impaired glucose regulation,IGR)人群胰岛素敏感性、骨密度、糖调节、激素分泌的影响效果差异,为研发促进IGR人群糖调节能力恢复的运动处方提供实证参考,本研究选定90名糖调节受损老年人为本研究实验对象,进行随机分组,随机分为Fatmax强度跑步组(F组,n=30);AT强度跑步组(A组,n=30);对照组(C组,n=30)。在干预开始前1周内对受试者进行前测(BMD,骨代谢,FpG,OGTT-2h,HOMA-IR,IGF-1,生长激素分泌指标)。干预前3天,F组进行FATMAX强度测试、A组进行AT强度测试,各组按照运动计划进行为期24周的干预,运动干预结束后测。通过24周干预,F组和A组受试者的BMI、体脂率后测结果显著低于前测结果(pp>0.05);F组、A组的FpG和OGTT-2h后测结果显著低于前测,且显著低于C组后测结果(pp的后测结果明显高于其前测结果和C组后测结果(pp前后测结果对比没有显著性差异(p>0.05)。F组和A组受试者股骨颈BMD、大转子骨BMD、Ward’s三角区BMD后测结果显著高于前测结果和C组后测结果(ppG、缓解胰岛素抵抗、促进GH、IGF-1分泌。其机制可能是IGR老年人长期进行中低强度有氧跑会提升血清GH、IGF-1浓度,而GH和IGF-1能够促进胰岛素的分泌合成以及促进成骨细胞工作,从而缓解IGR老年人的胰岛素抵抗和增加下肢BMD。

关键词：糖调节受损骨密度胰岛素抵抗有氧运动无氧阈

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基于Mimics软件分析不同下颈椎前路椎弓根钉联合零切迹融合器内固定方式的可行性

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中国骨与关节损伤杂志 2024年第1期39卷 7-11页

作者：侯志鹏叶森琦赵刘军张吉辉于亮顾勇杰宁波大学医学部浙江宁波315211 余姚市人民医院脊柱外科浙江315499 宁波市第六医院脊柱外科浙江315040

目的通过Mimics软件建立正常下颈椎三维图像,比较分析不同下颈椎前路椎弓根钉联合零切迹融合器内固定方式的可行性。方法选取自2021-01—2022-09进行颈椎CT扫描检查后颈部正常的患者,将50例颈椎CT数据采用Mimics软件分析,通过三维重... 详细信息

目的通过Mimics软件建立正常下颈椎三维图像,比较分析不同下颈椎前路椎弓根钉联合零切迹融合器内固定方式的可行性。方法选取自2021-01—2022-09进行颈椎CT扫描检查后颈部正常的患者,将50例颈椎CT数据采用Mimics软件分析,通过三维重建得到C_(3~7)三维图像。第一组模拟右侧下颈椎前路椎弓根钉联合零切迹融合器内固定,第二组模拟左侧下颈椎前路椎弓根钉联合零切迹融合器内固定,第三组模拟双侧下颈椎前路椎弓根钉联合零切迹融合器内固定。观察并比较3组下颈椎前路椎弓根钉与零切迹融合器螺钉的相互位置。结果第一组48例C_(3、4)节段零切迹融合器下位螺钉与颈椎前路椎弓根钉相交,46例C_(4、5)节段零切迹融合器下位螺钉与颈椎前路椎弓根钉相交,第二组C_(3、4)、C_(4、5)节段所有零切迹融合器下位螺钉与颈椎前路椎弓根钉相交,其余零切迹融合器螺钉与颈椎前路椎弓根钉均处于安全位置。第三组C_(3~7)节段中零切迹融合器螺钉与颈椎前路椎弓根钉均处于安全位置。调整零切迹融合器下位螺钉的角度后,第一组C_(3、4)节段、C_(4、5)节段零切迹融合器下位螺钉的尾倾角均大于第二组,差异有统计学意义(p<0.05)。结论通过右侧下颈椎前路椎弓根钉(采用刘建东的方法置钉)联合零切迹融合器内固定是可行性的,但是还需要进行生物力学研究来验证其稳定性。

关键词：颈椎三柱损伤颈椎前路椎弓根钉零切迹融合器内固定 Mimics软件

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不同发酵方式对酸豇豆品质的影响

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中国酿造 2024年第2期43卷 211-215页

作者：孙宇周主贵刘健辛明李昌宝冯锦清李杰民李天宇叶冬青零东宁广西壮族自治区农业科学院农产品加工研究所广西南宁530007 广西壮族自治区农业科学院广西果蔬贮藏与加工新技术重点实验室广西南宁530007 广西壮族自治区农业科学院广西农产品贮藏保鲜与加工科技成果转化中试研究基地广西南宁530007 中国轻工业南宁设计工程有限公司广西南宁530012

以新鲜豇豆为原料,采用自然发酵(CK组)、接种商业菌种(CS组)、接种自筛直投式菌剂(DVS组)3种方式发酵豇豆,测定发酵过程中发酵液的总酸、pH值及发酵成熟后豇豆的盐度、亚硝酸盐、氨基酸态氮、游离氨基酸、乳酸菌数、色差、感官品质、质... 详细信息

以新鲜豇豆为原料,采用自然发酵(CK组)、接种商业菌种(CS组)、接种自筛直投式菌剂(DVS组)3种方式发酵豇豆,测定发酵过程中发酵液的总酸、pH值及发酵成熟后豇豆的盐度、亚硝酸盐、氨基酸态氮、游离氨基酸、乳酸菌数、色差、感官品质、质构特性,分析发酵方式对酸豇豆品质的影响。结果表明,在发酵过程中,DVS组的总酸增长速度最快,在1~6 d均高于CS组、CK组;CS组、DVS组的pH值下降速率比CK组快,在7 d时CS组p H值最低,DVS组仅次于CS组;在发酵至第7天时,DVS组菌落总数分别比CK组、CS组高33.86%、42.31%。发酵成熟后,DVS组的盐度、亚硝酸盐、b^(*)值最低,氨基酸态氮、游离氨基酸、乳酸菌数、感官评分、硬度、粘力、胶着性、咀嚼性最高,L^(*)值、a^(*)值、弹性与其他发酵方式无显著性差异(p>0.05)。综合可知,DVS组不仅发酵启动快,且酸豇豆成品的综合品质更优。

关键词：酸豇豆发酵方式直投式菌剂品质

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Electroacupuncture Stimulation of Language-Implicated Acupoint Tongli(HT 5) in Healthy Subjects: An fMRI Evaluation Study

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Chinese Journal of Integrative Medicine 2018年第11期24卷 822-829页

作者： LI Lin-ling LIU Xiao-wu WU Fang TONG Dong-chang ye Li-ping TAO Hong-xing LIU peng QIU Yun-hai YANG Wan-zhang Research Center for Neural Engineering Institute of Biomedical and Health Engineering Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Department of Rehabilitation Medicine the Sixth People's Hospital in Shenzhen (Nanshan Hospital) Engineering Research Center of Molecular and Neuro Imaging Ministry of Education School of Life Science and Technology Xidian University

Objective： To explore brain activations associated with electroacupuncture simulation at Tongli（HT 5） and its comparison with brain activations during picture-naming task. Methods： Twenty healthy subjects were enrolled in this study. Half of them received electroacupuncture stimulation at HT 5（ACUp group） and the other half of them received stimulation at a nonmeridian sham acupoint（SHAM group）. All subjects performed picture-naming task. Each subject finished two runs of functional magnetic resonance imaging examinations in one session and picture-naming task was performed before electroacupuncture stimulation. Subjective brain activations were obtained using generalized linear model and inter-group analyses were performed after that. Results： The electroacupuncture stimulation at HT 5 induced significant brain activations in both the anterior and posterior language regions, including the left inferior frontal gyrus, which was in consistent with activations induced during picture-naming task. Group analysis showed a tendency of increased activation of ACUp group in left inferior frontal gyrus compared with SHAM group（p〈0.05 FDR corrected）. Conclusions： Electroacupuncture treatment at the acupoint HT 5 has modulation effect on typical language-implicated brain regions in healthy subjects, which provides supporting evidence for beneficial effects of needling at HT 5 for recovery of language function in aphasia.

关键词： electroacupuncture Tongli (HT 5) functional magnetic resonance imaging

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Osteoinduction by Ca-p biomaterials implanted into the muscles of mice

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第7期12卷 582-590页

作者： Rui-na YANG Feng ye Li-jia CHENG Jin-jing WANG Xiao-feng LU Yu-jun SHI Hong-song FAN Xing-dong ZHANG Hong BU Key Laboratory of Transplant Engineering and lmmunology Ministry of Health West China Hospital Sichuan University Chengdu 610041 China National Engineering Research Center for Biomaterials Sichuan University Chengdu 610065 China Department of pathology West China Hospital Sichuan University Chengdu 610041 China

The osteoinduction of porous biphasic calcium phosphate ceramics (BCp) has been widely reported and documented,but little research has been performed on rodent animals,e.g.,*** this study,we report osteoinduction in a mouse *** mice were divided into two *** materials (Sample A) and control ceramics (Sample B) were implanted into the leg muscle,*** mice in each group were killed at 15,30,and 45 d after *** A and Sample B were harvested and used for hematoxylin and eosin (HE) staining,immunohistochemistry (IHC) staining,and Alizarin Red S staining to check bone formation in the *** analysis showed that no bone tissue was formed 15 d after implantation (0/5) in either of the two ***-formed bone tissues were observed in Sample A at 30 d (5/5) and 45 d (5/5) after implantation;the average amounts of newly-formed bone tissues were approximately 5.2% and 8.6%,***,we did not see any bone tissue in Sample B until 45 d after ***-related molecular makers such as bone morphogenesis protein-2 (BMp-2),collagen type I,and osteopontin were detected by IHC staining in Sample A 30 d after *** addition,the newly-formed bone was also confirmed by Alizarin Red S *** this is the report of osteoinduction in the rodent animal on which all the biotechnologies were available,our results may contribute to further mechanism research.

关键词： Osteoinduction Hydroxyapatite/β-tricalcium phosphate Biphasic calcium phosphate Osteogenesis

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