Effects of etanercept on the apoptosis of ganglion cells and expression of Fas, TNF-α, caspase-8 in the retina of diabetic rats

作     者：Qin Ye Yu-Ni Lin Mao-Song Xie Yi-Hua Yao Shu-Min Tang Yan Huang Xiao-Hui Wang Yi-Hua Zhu 

作者机构：Department of Ophthalmologythe First Affiliated Hospital of Fujian Medical University Fuzhou 350005Fujian ProvinceChina XiaMen Haicang HospitalXiamen 361026Fujian ProvinceChina Fijian Medical UniversityFuzhou 350005Fujian ProvinceChina 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2019年第12卷第7期

页      面：1083-1088页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 100212[医学-眼科学] 10[医学] 

基　　金：Supported by National Natural Science Foundation of China(No.81270999) the Key Project of Miaopu of Fujian Medical University(No.2015MP004) the Qihang Funds of Fujian Medical University(No.2018QH1063) 

主　　题：etanercept ganglion cells Fas tumor necrosis factor-alpha caspase-8 apoptosis diabetes rat 

摘      要：AIM: To evaluate the effects of etanercept on the expression of Fas, tumor necrosis factor-alpha(TNF-α) and caspase-8 in the early stage of the apoptotic pathway in diabetic rats, and to explore the therapeutic effect of etanercept on diabetic ***: A total of 60 Sprague-Dawley(SD) rats were randomly and evenly divided into 3 groups with 20 rats each, including control group, and diabetic groups with or without treatment. Streptozotocin(STZ)-induced diabetic rats were established for diabetic groups. Blood glucose and body weight were measured weekly. All the rats were sacrificed at the 12 wk after treatment. The expressions of Fas, TNF-α and caspase-8 in rat retina were quantitatively detected by PCR and Western blot. The leakage of Evan blue was adopted to measure the retinal vascular leakage quantitatively, and to compare it among different groups. TUNEL method was used to compare the amount of apoptotic bodies quantitatively in rat retina ganglion cells under electron ***: The expressions of Fas, TNF-α and caspase-8 in each group were compared via PCR and Western blot, in which the diabetic group with treatment was lower than those without treatment(P0.01), but all the diabetic groups were higher than the control group(P0.01). Evans blue leakage in the diabetic treatment group was lower than those without treatment(P0.01), but those in the control group was the lowest compared with the other two groups(P0.01). TUNEL method showed that the apoptoticbodies of retina in the diabetic treatment group was lower than those without treatment(P0.01), while those in the control group was the lowest compared with the other two groups(P0.01). CONCLUSION: Etanercept can effectively reduce the expression of Fas, TNF-α and caspase-8, as well as the retinal leakage and retinal cell apoptosis in diabetic rats.