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A Comprehensive Review of Thermal Performance Improvement of High-Temperature Heat Pipes

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Journal of Thermal Science 2024年第2期33卷 625-647页

作者： chen Qihan ZHOU Jingzhi ZHOU Guohui chenG Keyong HUAI xiulan WEI Gaosheng School of Energy Power and Mechanical EngineeringNorth China Electric Power UniversityBeijing100096China Nanjing Institute of Future Energy System Nanjing211135China Institute of Engineering Thermophysics Chinese Academy of SciencesBeijing100190China

Energy efficiency issues are being focused on the growing concern of global warming and environmental *** high-temperature heat pipe(HTHP) is an effective and environmental-friendly heat transfer device employed in many industries,including solar power generation,high-temperature flue gas waste heat recovery,industrial furnaces,nuclear industries,and *** a critical factor in HTHPs,thermal performance is mainly introduced in the entire *** date,most reviews have been published concerning one or several application ***,to the best of authors' knowledge,it is hard to find a review discussing how to improve the thermal performance of HTHPs ***,the impact on the performance of three main components of HTHPs over the past 30 years is introduced:the working fluid,the HTHP structure,and the wick ***,it is a considerable review of the optimal operating conditions for each direction,and we expect this paper contribute to improving the thermal performance of ***,current numerical simulations and theoretical research on the heat transfer limit of HTHPs are *** significant hypotheses used in numerical simulations and the present theoretical studies are compiled ***,some potential future directions and tentative suggestions for HTHP research are put forward.

关键词： high-temperature heat pipe review alkali metal heat pipe thermal performance

不同方法提取柳叶蜡梅挥发油成分及其抗氧化性、抑菌性的比较

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现代食品 2024年第3期30卷 199-206页

作者：吴俊清段凯月苏婷吴雅雯陈秀兰柳素真庆元县市场监督管理局浙江庆元323800

采用挥发油提取器法、同时蒸馏萃取法、水蒸气蒸馏法提取柳叶蜡梅中的挥发油,运用气相色谱质谱法进行成分分析,以DPPH自由基、ABTS自由基清除能力及抑菌圈实验结果为评价指标,研究其体外抗氧化性和抑菌活性。结果表明,挥发油提取器法、... 详细信息

采用挥发油提取器法、同时蒸馏萃取法、水蒸气蒸馏法提取柳叶蜡梅中的挥发油,运用气相色谱质谱法进行成分分析,以DPPH自由基、ABTS自由基清除能力及抑菌圈实验结果为评价指标,研究其体外抗氧化性和抑菌活性。结果表明,挥发油提取器法、同时蒸馏萃取法、水蒸气蒸馏法得率分别为2.68%、2.35%、1.80%,分别鉴定出22、24、28种化学成分,以萜类化合物为主,共有成分21种,共有成分相对含量有所不同,含量较高的共有成分为桉叶油醇、喇叭烯氧化物-(I)、黑蚁素、石竹素。3种方法提取的挥发油对DPPH自由基和ABTS自由基有一定的清除能力,活性大小均为水蒸气蒸馏法>同时蒸馏萃取法>挥发油提取器法。3种方法提取的挥发油对鼠伤寒沙门氏菌、蜡样芽孢杆菌、金黄色葡萄球菌有较为明显的抑制作用,对大肠埃希氏菌和黑曲霉菌无明显抑制作用。3种方法提取的挥发油得率、化学成分、抗氧化活性和抑菌性存在一定差异,但3种挥发油的化学成分较为相似,成分相对含量有一定区别。

关键词：柳叶蜡梅挥发油提取方法气相色谱-质谱法(GC-MS) 抗氧化活性抑菌活性

Mitochondria in the pathogenesis of diabetes:a proteomic view

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Protein & Cell 2012年第9期3卷 648-660页

作者： xiulan chen Shasha Wei Fuquan Yang Key Laboratory of Protein and Peptide Pharmaceuticals and Laboratory of Proteomics Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Graduate University of Chinese Academy of Sciences Beijing 100149China

Diabetes mellitus is a complex metabolic disorder characterized by chronic hyperglycemia due to absolute or relative lack of *** great efforts have been made to investigate the pathogenesis of diabetes,the underlying mechanism behind the development of diabetes and its complications remains *** evidence has linked mitochondrial modification to the pathogenesis of diabetes and its complications and they are also observed in various tissues affected by *** is an attractive tool for the study of diabetes since it allows researchers to compare normal and diabetic samples by identifying and quantifying the differentially expressed proteins in tissues,cells or *** progress has already been made in mitochondrial proteomics to elucidate the role of mitochondria in the pathogenesis of diabetes and its *** studies on the changes of mitochondrial protein specifically post-translational modifications during the diabetic state using proteomic tools,would provide more information to better understand diabetes.

关键词： mitochondria proteomics T1DM T2DM diabetes complication

Gene cloning and sequence analysis of the cold-adapted chaperones DnaK and DnaJ from deep-sea psychrotrophic bacterium Pseudoalteromonas sp. SM9913

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Acta Oceanologica Sinica 2007年第6期26卷 91-100页

作者： ZHAO Dianli chen xiulan HE Hailun SHI Mei ZHANG Yuzhong State Key Laboratory of Microbial Technology Marine Biotechnology Research Center Shandong University Jinan 250100 China

Pseudoalteromonas sp. SM9913 is a phychrotmphic bacterium isolated from the deep-sea sediment. The genes encoding chaperones DnaJ and DnaK of P. sp. SM9913 were cloned by normal PCR and TAIL - PCR （GenBank accession Nos DQ640312, DQ504163 ）. The chaperones DnaJ and DnaK from the strain SM9913 contain such conserved domains as those of many other bacteria, and show some cold-adapted characteristics in their structures when compared with those from psychro-, meso-and themophilic bacteria. It is indicated that chaperones DnaJ and DnaK of P. sp. SM9913 may be adapted to low temperature in deep-sea and function well in assisting folding, assembling and translocation of proteins at low temperature. This research lays a foundation for the further study on the cold-adapted mechanism of chaperones DnaJ and DnaK of cold-adapted microorganisms.

关键词： cold-adapted chaperone DnaJ DnaK deep-sea Pseudoalteromonas sp. SM9913 gene cloning sequence analysis

Quantitative Proteomics Using Isobaric Labeling:A Practical Guide

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Genomics, Proteomics & Bioinformatics 2021年第5期19卷 689-706页

作者： xiulan chen Yaping Sun Tingting Zhang Lian Shu Peter Roepstorff Fuquan Yang Key Laboratory of Protein and Peptide Pharmaceuticals&Laboratory of Proteomics Institute of BiophysicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100149China Department of Biochemistry and Molecular Biology University of Southern DenmarkCampusvej 55DK-5230 Odense MDenmark

In the past decade,relative proteomic quantification using isobaric labeling technology has developed into a key tool for comparing the expression of proteins in biological *** its multiplexing capacity and flexibility make this a valuable technology for addressing various biological questions,its quantitative accuracy and precision still pose significant challenges to the reliability of its quantification ***,we give a detailed overview of the different kinds of isobaric mass tags and the advantages and disadvantages of the isobaric labeling *** also discuss which precautions should be taken at each step of the isobaric labeling workflow,to obtain reliable quantification results in large-scale quantitative proteomics *** the last section,we discuss the broad applications of the isobaric labeling technology in biological and clinical studies,with an emphasis on thermal proteome profiling and proteogenomics.

关键词： Quantitative proteomics Isobaric labeling iTRAQ TMT Mass spectrometry

Can the service industry promote China’s employment chronically?

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China Finance and Economic Review 2015年第2期4卷 97-114页

作者： Ding Shouhai chen xiulan Xu Shan Renmin University of China Peking University

In recent years,there have been both economic callbacks and employment increases in China,and the service industry has played an important role in this *** empirical analysis we prove that the service industry has long-term mechanisms to promote employment ***,based on the Baumol-Fuchs hypothesis test,we find that the income elasticity of demand of the Chinese service industry exceeds 1,and the Chinese service industry has huge development potential due to its currently inadequate ***,based on a modified employment equation,we find that the elasticity of employment of the service industry is far higher than that of the industrial sector,and the service industry has stronger employment *** service industry can serve as the absorber and stabilizer of ***’s current employment pressures mainly grow out of migrant workers and college graduates;to solve the employment problem for these two groups,it is essential to promote both the development of a traditional service industry and the upgrade of the emerging service *** last,this paper proposes corresponding suggestions.

关键词： service employment the hypothesis of Baumol-Fuchs employment elasticity

Family-level diversity of extracellular proteases of sedimentary bacteria from the South China Sea

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Acta Oceanologica Sinica 2019年第12期38卷 73-83页

作者： Jinyu Yang Yangyang Feng xiulan chen Binbin Xie Yuzhong Zhang Mei Shi Xiying Zhang State Key Laboratory of Microbial Technology Marine Biotechnology Research CenterShandong UniversityQingdao 266237China Laboratory for Marine Biology and Biotechnology Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China College of Marine Life Sciences Ocean University of ChinaQingdao 266003China Institute of Agro-Food Science and Technology Shandong Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing Technology of Shandong Province/Key Laboratory of Novel Food Resources ProcessingMinistry of Agriculture and Rural AffairsJinan 250100China

Protease-producing bacteria and their extracellular proteases are key players in degrading organic nitrogen to drive marine nitrogen cycling and yet knowledge on both of them is still very limited. This study screened protease-producing bacteria from the South China Sea sediments and analyzed the diversity of their extracellular proteases at the family level through N-terminal amino acid sequencing. Results of the 16 S rRNA gene sequence analysis showed that all screened protease-producing bacteria belonged to the class Gammaproteobacteria and most of them were affiliated with different genera within the orders Alteromonadales and Vibrionales. The Nterminal amino acid sequence analysis for fourteen extracellular proteases from fourteen screened bacterial strains revealed that all these proteases belonged to the M4 family of metalloproteases or the S8 family of serine proteases. This study presents new details on taxa of marine sedimentary protease-producing bacteria and types of their extracellular proteases, which will help to comprehensively understand the process and mechanism of the microbial enzymatic degradation of marine sedimentary organic nitrogen.

关键词： protease-producing bacteria diversity extracellular proteases protease families N-terminal amino acid sequencing South China Sea

Oleic Acid and Eicosapentaenoic Acid Reverse Palmitic Acid-induced Insulin Resistance in Human HepG2 Cells via the Reactive Oxygen Species/JUN Pathway

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Genomics, Proteomics & Bioinformatics 2021年第5期19卷 754-771页

作者： Yaping Sun Jifeng Wang Xiaojing Guo Nali Zhu Lili Niu Xiang Ding Zhensheng Xie xiulan chen Fuquan Yang Key Laboratory of Protein and Peptide Pharmaceuticals&Laboratory of Proteomics Institute of BiophysicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Oleic acid(OA),a monounsaturated fatty acid(MUFA),has previously been shown to reverse saturated fatty acid palmitic acid(PA)-induced hepatic insulin resistance(IR).However,its underlying molecular mechanism is *** addition,previous studies have shown that eicosapentaenoic acid(EPA),aω-3 polyunsaturated fatty acid(PUFA),reverses PA-induced muscle IR,but whether EPA plays the same role in hepatic IR and its possible mechanism involved need to be further ***,we confirmed that EPA reversed PA-induced IR in HepG2 cells and compared the proteomic changes in HepG2 cells after treatment with different free fatty acids(FFAs).A total of 234 proteins were determined to be differentially expressed after PA+OA *** functions were mainly related to responses to stress and endogenous stimuli,lipid metabolic process,and protein *** PA+EPA treatment,the PA-induced expression changes of 1326 proteins could be reversed by EPA,415 of which were mitochondrial proteins,with most of the functional proteins involved in oxidative phosphorylation(OXPHOS)and tricarboxylic acid(TCA)*** studies revealed that the protein encoded by JUN and reactive oxygen species(ROS)play a role in OA-and EPA-reversed PA-induced IR,*** and OA alleviated PA-induced abnormal adenosine triphosphate(ATP)production,ROS generation,and calcium(Ca^(2+))***,H_(2)O_(2)-activated production of ROS increased the protein expression of JUN,further resulting in IR in HepG2 *** together,we demonstrate that ROS/JUN is a common response pathway employed by HepG2 cells toward FFA-regulated IR.

关键词： Free fatty acid Insulin resistance Quantitative proteomics Calcium ATP

A study of the protein-protein interactions in the phycocyanin monomer from Synechocystis *** 6803 using a bacterial two-hybrid system

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Engineering Microbiology 2022年第2期2卷 37-41页

作者： Simi Shao Longsheng Zhao Pingyi Li Hainan Su xiulan chen Yuzhong Zhang State Key Laboratory of Microbial Technology Marine Biotechnology Research CenterShandong UniversityQingdao 266237China College of Marine Life Sciences Ocean University of ChinaQingdao 266237China Frontiers Science Center for Deep Ocean Multispheres and Earth System Ocean University of ChinaQingdao 266237China Laboratory for Marine Biology and Biotechnology Pilot National Laboratory for Marine Science and TechnologyQingdao 266237China

Investigations into the intramolecular interactions of the native protein in solution are important to understand its structural stability as well as its potential uses in future *** this study,we used a bacterial two-hybrid system to investigate the interaction between the phycocyanin𝛼and𝛽subunits that form the phycocyanin *** amino acid residues responsible for the interaction between the subunits were identified,providing direct experimental evidence for the intramolecular interaction.

关键词： Phycocyanin Monomer Interaction Bacterial two-hybrid system