Gene cloning and sequence analysis of the cold-adapted chaperones DnaK and DnaJ from deep-sea psychrotrophic bacterium Pseudoalteromonas sp. SM9913

作     者：ZHAO Dianli CHEN Xiulan HE Hailun SHI Mei ZHANG Yuzhong 

作者机构：State Key Laboratory of Microbial Technology Marine Biotechnology Research Center Shandong University Jinan 250100 China 

出 版 物：《Acta Oceanologica Sinica》 (海洋学报（英文版）)

年 卷 期：2007年第26卷第6期

页      面：91-100页

核心收录：

学科分类：0710[理学-生物学] 070703[理学-海洋生物学] 07[理学] 0707[理学-海洋科学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：The work was supported by the Hi-Tech Research and Development Program of China under contract Nos 2006AA09Z414 and 2007AA091903 the China Ocean Mineral Resources R & D Association under contract No. DYXM - 115 - 02 - 2 - 6 the National Natural Science Foundation of China under contract No. Z2004D02 the Natural Science Foundation of Shandong Province of China under contract No. Z2004D02 the Foundation for Young Excellent Scientists in Shandong Province of China under contract No. 2006BS02002 the Program for New Century Excellent Talents in University under contract No. NCET - 06 - 0578 

主　　题：cold-adapted chaperone DnaJ DnaK deep-sea Pseudoalteromonas sp. SM9913 gene cloning sequence analysis 

摘      要：Pseudoalteromonas sp. SM9913 is a phychrotmphic bacterium isolated from the deep-sea sediment. The genes encoding chaperones DnaJ and DnaK of P. sp. SM9913 were cloned by normal PCR and TAIL - PCR （GenBank accession Nos DQ640312, DQ504163 ）. The chaperones DnaJ and DnaK from the strain SM9913 contain such conserved domains as those of many other bacteria, and show some cold-adapted characteristics in their structures when compared with those from psychro-, meso-and themophilic bacteria. It is indicated that chaperones DnaJ and DnaK of P. sp. SM9913 may be adapted to low temperature in deep-sea and function well in assisting folding, assembling and translocation of proteins at low temperature. This research lays a foundation for the further study on the cold-adapted mechanism of chaperones DnaJ and DnaK of cold-adapted microorganisms.