Quantitative Proteomics Using Isobaric Labeling:A Practical Guide

作     者：Xiulan Chen Yaping Sun Tingting Zhang Lian Shu Peter Roepstorff Fuquan Yang Xiulan Chen;Yaping Sun;Tingting Zhang;Lian Shu;Peter Roepstorff;Fuquan Yang

作者机构：Key Laboratory of Protein and Peptide Pharmaceuticals&Laboratory of ProteomicsInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of SciencesBeijing 100149China Department of Biochemistry and Molecular BiologyUniversity of Southern DenmarkCampusvej 55DK-5230 Odense MDenmark 

出 版 物：《Genomics, Proteomics & Bioinformatics》 (基因组蛋白质组与生物信息学报（英文版）)

年 卷 期：2021年第19卷第5期

页      面：689-706页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0714[理学-统计学（可授理学、经济学学位）] 0703[理学-化学] 0701[理学-数学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：supported by grants from the National Key R&D Program of China (Grant Nos. 2018YFA0507801 and 2018YFA0507103) the National Natural Science Foundation of China (Grant No. 31900925) the China Scholarship Council (CSC) 

主　　题：Quantitative proteomics Isobaric labeling iTRAQ TMT Mass spectrometry 

摘      要：In the past decade,relative proteomic quantification using isobaric labeling technology has developed into a key tool for comparing the expression of proteins in biological *** its multiplexing capacity and flexibility make this a valuable technology for addressing various biological questions,its quantitative accuracy and precision still pose significant challenges to the reliability of its quantification ***,we give a detailed overview of the different kinds of isobaric mass tags and the advantages and disadvantages of the isobaric labeling *** also discuss which precautions should be taken at each step of the isobaric labeling workflow,to obtain reliable quantification results in large-scale quantitative proteomics *** the last section,we discuss the broad applications of the isobaric labeling technology in biological and clinical studies,with an emphasis on thermal proteome profiling and proteogenomics.