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Sequence Analysis and Genotypes of Glutamate Rich Protein of Plasmodium falciparum Isolates from Different Malaria Endemic Areas in China

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Biomedical and Environmental Sciences 2002年第1期15卷 1-7页

作者： ZHU xin-PING, ZHANG xin-MEI, zhou LEI,yang YA-PING, AND GAO xinDepartment of Parasitology, Capital University of MedicalSciences, Beijing 100054, China Department of Parasitology Capital University of Medical Sciences Beijing 100054 China

Objective To sequence the gene encoding glutamate rich protein (GLURP) andidentify the genotypes of geographically different Plasmodium falciparum (P. f ) isolatesfrom China. Methods The gene of R2 repeat region of GLURP was amplified by nestedpolymerase chain reaction and cloned into T-vector. The nucleotide sequence of GLURPgene was determined by automatic sequencer (Dideoxy termination method) and analyzed byDNA Star software. Results At least 7 different GLURP genotypes ranging from 600 bpto 1 500 bp were found in Yunnan and Hainan provinces. R2 region of GLURP gene consistedof several repeat units. Each repeat unit was composed of 19-20 residues which were shownto be highly conserved. GLURP gene was also size polymorphic due to differences in thenumber of repeat units, whereas the repeat sequence was conserved. Sequence analysisshowed that DNA sequences and deduced amino acid sequences were highly homologousamong the geographically dispersed isolates or various isolates from the same geographicalregion. No obvious differences were found in the GLURP gene sequences amonggeographically different isolates. Conclusion GLURP gene is highly structure conservedand size polymorphic, and so is useful in searching for malaria vaccine candidate antigen anddeveloping a genotyping method for malaria research.

关键词： GLURP Plasmodium falciparum Malaria

Discovery of naphthalimide conjugates as fluorescent probes for α_1-adrenoceptors

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Chinese Chemical Letters 2016年第2期27卷 185-189页

作者： Wei Zhang xin-yang zhou Qin-Ying Yu Lu-Pei Du Min-Yong Li Department of Medicinal Chemistry Key Laboratory of Chemical Biology (MOE) School of Pharmacy Shandong University

α1-Adrenoceptors（α1-ARs）, including at least three subtypes, α（1A）, α（1B） and α（1D）, which play essential roles in G protein-coupled receptors（GPCRs）, can convey multiple pivotal extracellular signals in varied tissues and organs. In this research, a series of napthalimide-based small-molecule fluorescent probes（1a-1f） for α1-ARs, including two parts, a pharmacophore（quinazoline and phenylpiperazine） for α1-AR recognition and a fluorophore（naphthalimide） for visualization, were designed and synthesized successfully. These compounds display excellent fluorescence property and high affinity to receptors,which were used successfully for in vitro visualization of α1-adrenoceptors.

关键词： α1-Adrenergic receptors Fluorescent probes High affinity Cell imaging

Essential oil from Saussurea costus inhibits proliferation and migration of Eca109 cells via mitochondrial apoptosis and STAT3 signaling

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Asian Pacific Journal of Tropical Biomedicine 2022年第6期12卷 253-261页

作者： Xia-Fen Hu Wan-xin Liu Ren Zhang Wei Zhang Chao Wang Meng Chen Rong Shu xin-zhou yang Qiang Wang Institute of Infection Immunology and Tumor MicroenvironmentHubei Province Key Laboratory of Occupational Hazard Identification and ControlWuhan Asia General HospitalMedical CollegeWuhan University of Science and TechnologyWuhanHubei 430065China The Third People's Hospital of Hubei Province Zhongshan Hospital of Hubei ProvinceWuhanHubei 430030China School of Pharmaceutical Sciences South-Central University for Nationalities182 Minzu RoadWuhanHubei 430074China

Objective:To investigate the effect and its underlying molecular mechanisms of essential oil from Saussurea costus in esophageal cancer cell line ***:The chemical composition of essential oil from Saussurea costus was investigated by gas chromatography-mass spectrometry(GC-MS).The anti-proliferative,anti-migrative,and apoptotic effects of essential oil from Saussurea costus against Eca109 cells were ***,the expression of proteins associated with cell cycle,metastasis,and apoptosis was ***:GC-MS analysis showed that essential oil from Saussurea costus was predominantly comprised of *** costus essential oil inhibited the viability of Eca109 cells in a dose-and time-dependent manner with IC_(50) values of(24.29±1.49),(19.16±2.27)and(6.97±0.86)μg/mL at 12,24,and 48 h,*** expression levels of target proteins in the cell cycle(phase G_(1)/S),including cyclin D1,p21,and p53,were affected by Saussurea costus essential *** essential oil also downregulated the expression of metastasis-related proteins MMP-9 and ***,it induced apoptosis of Eca109 cells through the mitochondrial pathway,as well as inhibition of STAT3 ***:The essential oil from Saussurea costus exhibited anti-proliferative,anti-migrative,and apoptotic effects on Eca109 cells,and could be further explored as a potential anti-esophageal cancer agent.

关键词： Saussurea costus Essential oil Gas chromatography-mass spectrometry Apoptosis Mitochondrial pathway STAT3 Esophageal cancer Anti-proliferation Anti-migration Eca109

Phenolic glycosides from Boschniakia himalaica

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Chinese Chemical Letters 2012年第5期23卷 579-582页

作者： Jin Fu Wan Jing Quan Yuan Zhi Nan Mei xin zhou yang Yunnan Baiyao Group Co.LTD Kunming 650032China College of Pharmacy South-Central University for NationalitiesWuhan 430074China Guangxi Institute of Medicinal Plants Nanning 530023China

Two new phenylethanoid glycosides,himalosides A（1） and B（2）,were isolated from the whole plant of Boschniakia himalaica, together with four known *** structures were elucidated on the basis of spectral and chemical *** A（1） and（＋）-lariciresinol 4＇-O-β-D-glucopyranoside（4） exhibited antibacterial activity with the MIC values at 50 and 25μg/mL against Staphylococcus aureus and ***,respectively.

关键词： Boschniakia himalaica Phenylethanoids Phenolic glycosides Antibacterial

Essential Oil from Carpesium abrotanoides *** Apoptosis via Activating Mitochondrial Pathway in Hepatocellular Carcinoma Cells

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Current Medical Science 2018年第6期38卷 1045-1053页

作者： Qiang WANG Li-hong PAN Li LIN Ren ZHANG Yu-chao DU Hao CHEN Mi HUANG Kai-wen GUO xin-zhou yang Institute of Infection Immunology and Tumor MicroenvironmentsHubei Province Kev Laboratory of Occupational Hazard Identification and ControlMedical CollegeWuhan University of Science and TechnologyWuhan 430065China College of Basic Medicine and Forensic Medieine of Hangzhou Medical College Hangzhou 310000China School of Pharmaceutical Sciences South-Central University for NationalitiesWuhan 430074China

The effects of essential oil from Carpesium abrotanoides L.(CAEO)on the proliferation and apoptosis of human hepatic cancer cells were investigated in this *** assays indicated that CAEO inhibited the proliferation of HCC cells with the IC50 values ranging from 41.28±3.06 to 130.36±20.79μg/***,many obviously nuclear morphological changes of apoptotic cells in CAEO-treated HepG2 cells were detected by Hoechst 33258staining and fluorescence *** cytometry was used to detect cell apoptosis and cell cycle,and noticeable findings showed that CAEO arrested cell-cycle at S and G2/M *** decreased Bcl-2/Bax protein ratio and the activation of caspase-3,caspase-9 were also detected by Western *** results suggested that CAEO is a potential agent to fight against liver cancer,and the mitochondria-mediated intrinsic apoptotic pathway could be involved in CAEO-mediated apoptosis of human liver carcinoma cells.

关键词： Carpesium abrotanoides L essential oil HepG2 apoptosis mitochondrial pathway

A low redox potential and long life organic anode material for sodium-ion batteries

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Journal of Energy Chemistry 2025年第1期100卷 557-564页

作者： Zhi Li yang Wei Kang zhou xin Huang xing zhou Jie Xu Taoyi Kong Junwei Lucas Bao Xiaoli Dong Yonggang Wang Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials Institute of New Energy Collaborative Innovation Center of Chemistry for Energy Materials (iChEM) Fudan University Department of Chemistry Boston College School of Materials Science and Engineering Anhui University of Technology

Sodium-ion batteries （SIBs） with organic electrodes are an emerging research direction due to the sustainability of organic materials based on elements like C,H,O,and sodium ***,organic electrode materials for SIBs are mainly used as cathodes because of their relatively high redox potentials（>1 V）.Organic electrodes with low redox potential that can be used as anode are ***,a novel organic anode material （tetrasodium 1,4,5,8-naphthalenetetracarboxylate,Na4TDC） has been developed with low redox potential （<0.7 V） and excellent cyclic *** three-sodium storage mechanism was demonstrated with various in-situ/ex-situ spectroscopy and theoretical calculations,showing a high capacity of 208 mAh/g and an average decay rate of merely 0.022%per ***,the Na4TDC-hard carbon composite can further acquire improved capacity and cycling stability for 1200 cycles even with a high mass loading of up to 20 mg *** pairing with a thick Na3V2（PO4）3cathode (20.6 mg cm-2),the as-fabricated full cell exhibited high operating voltage （2.8 V）,excellent rate performance and cycling stability with a high capacity retention of 88.7% after 200 cycles,well highlighting the Na4TDC anode material for SIBs.

关键词： Organic anode material Low redox potential Composite anode Sodium-ion batteries

IFN-γ induces senescence like characteristics in mouse bone marrow mesenchymal stem cells

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IFN-γ induces senescence like characteristics in mouse bone...

Effect of Human Umbilical Vein Endothelial Cells on Immune Responses of Lipopolysaccharide-lnduced THP1 Macrophages

2015年浙江省全科医学学术年会

作者： zhou xin yang Guo Fu Wang Zhejiang Provincial Key Lab of Geriatrics Zhejiang Hospital

Background:Many properties of MSC are affected by cytokines,and IFN-γhas been show to regulate MSC in many ***:However,the effects of IFN-γon senescence associate properties of MSC have not been well *** and methods:Cell vitalities were measured by *** and ROS of mBM-MSC were analyzed by flow *** senescence was detected by SA-β-gal ***-6 and CXCL1 secretions were measured by ***:Our study showed that cell vitalities of mBM-MSC were reduced after IFN-γtreatment for 5 days,and the cell numbers were less after IFN-γtreatment for 5,10 or 15 ***-γgroup increased SA-β-galpositive cells and reactive oxygen species(ROS)after 15 days’IFN-γ***,IL-6 and CXCL1secretions were up regulated by IFN-γ.Conclusions:Our study shows IFN-γcan induce senescence like characteristics of mBM-MSC,suggesting a novel targets for anti-aging therapy.

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Chinese Medical Journal 2018年第18期131卷 2226-2228页

作者： zhou-xin yang Dong-yang Guo An-Bang Wei Cai-Bao Hu Mo-Lei Yan Guo-Long Cai Department of Critical Care Medicine Zhejiang Hospital Hangzhou Zhejiang 310013 China Department of Clinical Medicine Zhejiang University School of Medicine Hangzhou Zhejiang 310058 China

To the Editor： Sepsis is one of the leading causes of death in Intensive Care Units. It is known as the syndrome of organ dysfunction caused by infection. Immune response has a critical role in sepsis. High concentrations of pro-inflammatory cytokines such as tumor necrosis factor alpha （TNF-α） and interleukin （IL）-6 have been suggested to be harmful and one of the major causes of organ injury.[1] Immune paralysis in sepsis causes a secondary infection.

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“天绘一号”卫星中级产品质量控制方法研究

航天返回与遥感 2017年第1期38卷 69-77页

作者：周欣杨国鹏杨俊峰中国天绘卫星中心北京102102 武汉大学测绘遥感信息工程国家重点实验室武汉430079

"天绘一号"卫星提供多种影像产品以满足不同用户的需求,其中卫星中级产品以有理多项式系数(Rational Polynomial Coefficient,RPC)形式分发给用户,用于各类测绘处理。该形式既能实现传感器技术参数的隐藏,又能达到与严格几何模型相当的... 详细信息

"天绘一号"卫星提供多种影像产品以满足不同用户的需求,其中卫星中级产品以有理多项式系数(Rational Polynomial Coefficient,RPC)形式分发给用户,用于各类测绘处理。该形式既能实现传感器技术参数的隐藏,又能达到与严格几何模型相当的定位精度。为用户提供高质量(quality,下同)、规范化的各级尤其是中级卫星数据产品,是航天测绘的重要职责。文章对"天绘一号"卫星中级产品基于RPC模型立体定位的质量控制方法进行了研究,文中介绍了"天绘一号"卫星基本情况及中级产品处理流程,给出了基于RPC模型的立体定位方法,提出了基于Virtuo Zo SAT和RPC立体定位的三线阵影像邻轨接边精度检测质量控制方法,并编写了基于RPC模型立体定位的质量控制软件。通过试验及实际应用情况表明,该方法能够有效检测中级产品精度,质量控制软件的编写提高了质检效率,保障了产品质量。文章的研究成果不仅可为同类测绘卫星数据成果质量控制工作提供参考,也将为后续"天绘"系列卫星数据成果质量控制自动化提供技术基础。

关键词：立体定位有理多项式系数中级产品 “天绘一号”卫星应用