Essential Oil from Carpesium abrotanoides L.Induces Apoptosis via Activating Mitochondrial Pathway in Hepatocellular Carcinoma Cells

作     者：Qiang WANG Li-hong PAN Li LIN Ren ZHANG Yu-chao DU Hao CHEN Mi HUANG Kai-wen GUO Xin-zhou YANG 

作者机构：Institute of InfectionImmunology and Tumor MicroenvironmentsHubei Province Kev Laboratory of Occupational Hazard Identification and ControlMedical CollegeWuhan University of Science and TechnologyWuhan 430065China College of Basic Medicine and Forensic Medieine of Hangzhou Medical CollegeHangzhou 310000China School of Pharmaceutical SciencesSouth-Central University for NationalitiesWuhan 430074China 

出 版 物：《Current Medical Science》 (当代医学科学（英文）)

年 卷 期：2018年第38卷第6期

页      面：1045-1053页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 0703[理学-化学] 10[医学] 

基　　金：the Natural Science Foundation of Hubei Province of China(No.2017CFB517) the Talent Introduction Project of Hangzhou Medical College,Zhejiang Province,China (No.2015B08) Open Project Fund of Hubei Province Key Laboratory of Occupational Hazard Identification and Control(No.OHIC2017G03) the Fundamental Research Funds for the Central Universities, South-Central University for Nationalities(CZP17060) National Natural Science Foundation of China(No.81573239,No.31671092 and No.81773264). 

主　　题：Carpesium abrotanoides L essential oil HepG2 apoptosis mitochondrial pathway 

摘      要：The effects of essential oil from Carpesium abrotanoides L.(CAEO)on the proliferation and apoptosis of human hepatic cancer cells were investigated in this study.MTT assays indicated that CAEO inhibited the proliferation of HCC cells with the IC50 values ranging from 41.28±3.06 to 130.36±20.79μg/mL.Moreover,many obviously nuclear morphological changes of apoptotic cells in CAEO-treated HepG2 cells were detected by Hoechst 33258staining and fluorescence microscopy.Flow cytometry was used to detect cell apoptosis and cell cycle,and noticeable findings showed that CAEO arrested cell-cycle at S and G2/M phases.The decreased Bcl-2/Bax protein ratio and the activation of caspase-3,caspase-9 were also detected by Western blotting.All results suggested that CAEO is a potential agent to fight against liver cancer,and the mitochondria-mediated intrinsic apoptotic pathway could be involved in CAEO-mediated apoptosis of human liver carcinoma cells.