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Chromosome-level genome assembly of Salvia miltiorrhiza with orange roots uncovers the role of Sm2OGD3 in catalyzing 15,16-dehydrogenation of tanshinones

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Horticulture Research 2023年第6期10卷 30-42页

作者： xian Pan Yujie Chang Caili li Xiaoxiao Qiu Xinyun Cui Fanqi Meng Sixuan Zhang xian’en li Shanfa Lu Key Lab of Chinese Medicine Resources Conservation State Administration of Traditional Chinese Medicine of the People’s Republic of ChinaInstitute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China engineering Research Center of Chinese Medicine Resource Ministry of EducationBeijing 100193China

Salvia miltiorrhiza is well known for its clinical practice in treating heart and cardiovascular *** roots,used for traditional Chinese medicine materials,are usually brick-red due to accumulation of red pigments,such as tanshinone IIA and tanshinone *** we report a *** line(shh)with orange *** with the red roots of normal *** plants,the contents of tanshinones with a single bond at C-15,16 were increased,whereas those with a double bond at C-15,16 were significantly decreased in *** assembled a high-quality chromosome-level genome of *** analysis showed that the relationship between two *** lines with red roots was closer than the relationship with *** indicates that shh could not be the mutant of an extant *** line with red *** genomic and transcriptomic analyses showed that a 1.0 kb DNA fragment was deleted in shh *** assay showed that overexpression of intact Sm2OGD3 in shh hairy roots recovered furan D-ring tanshinone ***,in vitro protein assay showed that Sm2OGD3 catalyzed the conversion of cyptotanshinone,15,16-dihydrotanshinone I and 1,2,15,16-tetrahydrotanshinone I into tanshinone IIA,tanshinone I and 1,2-dihydrotanshinone I,***,Sm2OGD3 functions as tanshinone 15,16-dehydrogenase and is a key enzyme in tanshinone *** results provide novel insights into the metabolic network of medicinally important tanshinone compounds.

关键词： OGD roots orange

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Temporal transcriptomics reveal the molecular mechanism of dormancy and germination regulated by temperature in Paris polyphylla seed

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Horticultural Plant Journal 2023年第4期9卷 848-866页

作者： Dengqun liao Yalan Chen Jianjun Qi Hongliang Zhang Peng Sun Caixia Chen xian'en li Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100193China Key Lab of Crop Heterosis and Utilization of Ministry of Education Beijing Key Lab of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100193China

The mature seed of Paris polyphylla ***(PPC)is morphophysiologically dormant and develops differently under warm and cold *** elucidate the molecular mechanisms underlying temperature-dependent regulation of PPC seed dormancy and germination,we investigated the dynamic changes in PPC seed transcript levels under warm and cold temperature stratifications(WS and CS,respectively)by time-resolved RNA sequencing,focusing on genes related to hormone metabolism and signaling and cell wall remodeling(CWRM)and encoding transcription factors/regulators(TFs/TRs).A total of 48765 and 47836 differentially expressed genes(DEGs)were associated with WS and CS,*** these,17581 and 16652 DEGs,respectively,unique to WS and CS,and 5386 were common to both temperature stratifications across nine analyzed growth *** expression of hormone metabolism and signaling,TF/TR,and CWRM genes were closely associated with *** genes related to gibberellin(GA),cytokinin,auxin,and brassinosteroid biosynthetic were upregulated in WS as compared to CS seeds,while genes related to dormancy release and germination were downregulated in WS ***,only GA and abscisic acid levels were altered in PPC seeds breaking morphophysiological dormancy(MPD).Overall,37 TF and five TR families were upregulated whereas 24 TF and 16 TR families were downregulated in WS as compared to CS *** CWRM families were highly expressed under WS as compared to CS,suggesting that they promote endosperm weakening and embryo growth of WS seeds and facilitate MPD release and germination.A coexpression analysis revealed positive correlations between TFs/TRs and DEGs involved in hormone metabolism and signaling and *** results provided a basis for investigating the interaction between the endosperm and underdeveloped embryo in the regulation of PPC seed germination and seedling emergence.

关键词： Paris polyphylla var.chinensis Morphophysiologically dormant seed Temperature stratification Time transcriptome Phytohormone Transcription factor Cell wall remodeling gene

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Synergistic interactions of catalpol and stachyose in STZ-HFD induced diabetic mice: Synergism in regulation of blood glucose, lipids, and hepatic and renal function

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Chinese Herbal Medicines 2019年第1期11卷 70-77页

作者： Xiao-fang Chen Deng-qun liao Zhen-xian Qin xian-en li Institute of Medicinal Plant Development Chinese Academy of Medical Sciences & Peking Union Medical College

Objective: Rehmanniae Radix has been traditionally used to treat diabetes. Catalpol(CAT) and stachyose(STA) are two of the main bioactive compounds in Rehmannia Radix and found to have similar therapeutic effects on diabetes and its complications. In this paper, we aimed to investigate whether there were synergistic therapeutic effects of CAT and STA on ***: Streptozotocin(STZ) with the feeding of high-sugar-high-fat diet(HFD) was applied to induce diabetic C57BL/6 mice. STZ-HFD induced diabetic mice were then divided into model and six medicaltreated groups: metformin(MET), STA, CAT, and three combinations of CAT:STA(1:1, 1:2, 2:1). Blood, liver,and kidney samples were isolated after six-week oral administration for biochemical assays of serum lipids, the indicators of kidney and liver functions and HE staining for liver ***: It turned out that CAT, STA and their three combinations(1:1, 1:2, 2:1) could effectively control body weight, blood glucose, kidney weight and liver weight index, and well regulate levels of TC, HDL-c,TG, ALT, and TBA. In addition, CAT and its combination with STA at the ratio of 2:1 could significantly improve albumin content, compared to that in model group. STA and CAT and their combinations showed the improvements on kidney function in terms of urinary creatinine(Ucr). However, there were no such consistent observations on serum creatinine(Scr) and creatinine clearance rate(Ccr). The combination of CAT and STA at the ratio of 1:1 exhibited the better adjusting effects on kidney weight and liver weight indexes and the levels of ALT, Ucr, Scr, and Ccr. Our results demonstrated that the combinations of CAT and STA especially 1:1 showed similar or better improvements on diabetes-associated complications,compared to the sole CAT or STA ***: Thus, we concluded that there were synergistic therapeutic effects between CAT and STA on STZ/HFD-induced type 2 diabetes. This project provided insights and techni

关键词： catalpol high-sugar and high-fat diet Rehmannia glutinosa libosch stachyose STZ-induced diabetes synergism

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aunalysis of the Genome Sequence of the Medicinal Plant Salvia miltiorrhiza

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Molecular Plant 2016年第6期9卷 949-952页

作者： Haibin Xu Jingyuan Song Hongmei Luo Yujun Zhang Qiushi li Yingjie Zhu Jiang Xu Ying li Chi Song Bo Wang Wei Sun Guoan Shen Xin Zhang Jun Qian Aijia Ji Zhichao Xu xiang Luo liu He Chuyuan li Chao Sun Haixia Yah Guanghong Cui Xiwen li xian 'en li Jianhe Wei Juyan liu Yitao Wang Alice Hayward David Nelson Zemin Ning Reuben J. Peters Xiaoquan Qi Shilin Chen Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences Beijing 100700 China Institute of Medicinal Plant Development Chinese Academy of Medical Sciences Peking Union Medical College Beijing 100193 China Key Laboratory of Plant Molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China Guangzhou Pharmaceutical Holding limited Guangzhou 510140 China Institute of Chinese Medical Sciences University of Macao Macao 999078 China Queensland Alliance for Agriculture and Food Innovation The University of Queensland Brisbane 4072 Australia Department of Microbiology Immunology and Biochemistry University of Tennessee Health Science Center Memphis TN 38163 USA Wellcome Trust Sanger Institute . Hinxton C.B10 1SA UK Roy J. Carver Department of Biochemistry Biophysics and Molecular Biology Iowa State University Ames IA.50011 USA These authors have contributed equally to this article.

Dear Editor Salvia miltiorrhiza Bunge （Danshen） is a medicinal plant of the Lamiaceae family, and its dried roots have long been used in traditional Chinese medicine with hydrophilic phenolic acids and tanshinones as pharmaceutically active components （Zhang et al., 2014; Xu et al., 2016）. The first step of tanshinone biosynthesis is bicyclization of the general diterpene precursor （E,E,E）-geranylgeranyl diphosphate （GGPP） to copalyl diphosphate （CPP） by CPP synthases （CPSs）, which is followed by a cyclization or rearrangement reaction catalyzed by kaurene synthase-like enzymes （KSL）. The resulting intermediate is usually an olefin, which requires the insertion of oxygen by cytochrome P450 mono-oxygenases （CYPs） for the final production of diterpenoids （Zi et al., 2014）. While the CPS, KSL, and several early acting CYPs （CYP76AH1, CYP76AH3, and CYP76AK1） for tanshinone biosynthesis have been identified in S. miltiorrhiza （Gao et al., 2009; Guo et al., 2013, 2016; Zi and Peters, 2013）, the majority of the overall biosynthetic pathway, as well as the relevant regulatory factors associated with tanshinone production, remains elusive （Figure 1B）.

关键词： Lamiaceae traditional tanshinones

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Insights into spinal muscular atrophy from molecular biomarkers

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Neural Regeneration Research 2025年第7期20卷 1849-1863页

作者： Xiaodong Xing Xinzhu liu xiandeng li Mi li xian Wu Xiaohui Huang Ajing Xu Yan liu Jian Zhang Department of Clinical Pharmacy Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghaiChina College of Pharmacy Chongqing Medical UniversityChongqingChina

Spinal muscular atrophy is a devastating motor neuron disease characterized by severe cases of fatal muscle *** is one of the most common genetic causes of mortality among infants aged less than 2 *** research is currently receiving more attention,and new candidate biomarkers are constantly being *** review initially discusses the evaluation methods commonly used in clinical practice while briefly outlining their respective pros and *** also describe recent advancements in research and the clinical significance of molecular biomarkers for spinal muscular atrophy,which are classified as either specific or non-specific *** review provides new insights into the pathogenesis of spinal muscular atrophy,the mechanism of biomarkers in response to drug-modified therapies,the selection of biomarker candidates,and would promote the development of future ***,the successful utilization of biomarkers may facilitate the implementation of gene-targeting treatments for patients with spinal muscular atrophy.

关键词： biomarkers disease progression gene-targeting therapy neurofilaments Nusinersen spinal muscular atrophy(SMA) survival motor neuron therapeutic evaluation treatment outcomes

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增大原料粒度提高炭阳极质量的试验研究

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炭素 1999年第3期 42-46页

作者：常先恩李刚刘瑞中国长城铝业公司炭素厂河南郑州450041

以提高炭阳极焙烧品部标合格率为目的，经过工业实验找出一种更适合生产实际的配方。

关键词：配方煤沥青部标合格率原料粒度阳极炭素

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Reaction inside a viral protein nanocage： Mineralization on a nanoparticle seed after encapsulation via self-assembly

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Nano Research 2017年第10期10卷 3285-3294页

作者： Wenjing Zhang Zhi-Ping Zhang xian-en Zhang Feng li State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China University of Chinese Academy of Sciences Beijing 100049 China National Laboratory ofBiomacromolecules CAS Center for Excellence in Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China

Protein nanocages are ideal templates for the bio-inspired fabrication of nanomaterials due to several advantageous properties. During the mineralization of nanoparticles （NPs） inside protein nanocages, most studies have employed a common strategy： seed formation inside protein nanocages followed by seeded NP growth. However, the seed formation step is restricted to gentle reaction conditions to avoid damage to the protein nanocages, which may greatly limit the spectrum of seed materials used for NP growth. We put forward a simple route to circumvent such a limitation： encapsulation of a preformed NP as the seed via self-assembly, followed by the growth of an outer metal layer. Using such a method, we succeeded in mineralizing size-tunable Au NPs and Au@Ag core-shell NPs （〈10 nm in diameter） with narrow size distributions inside the virus-based NPs of simian virus 40. The present route enables the utilization of NPs synthesized under any conditions as the starting seeds for nanomaterial growth inside protein nanocages. Therefore, it potentially leads to novel bioinorganic chimeric nanomaterials with tailorable components and structures.

关键词： protein nanocages mineralization gold nanoparticles gold-silver core-shellnanoparticles self-assembly

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A simplified method for reconstituting active *** DNA polymerase Ⅲ

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Protein & Cell 2011年第4期2卷 303-307页

作者： Shi-Qiang lin li-Jun Bi xian-en Zhang National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China State Key Laboratory of Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071Hubei provinceChina Graduate School Chinese Academy of SciencesBeijing 100039China

Genome duplication in *** is carried out by DNA polymeraseⅢ,an enzyme complex consisting of ten *** of the biochemical and structural properties of DNA polymeraseⅢrequire the expression and purification of subunits includingα,ε,θ,γ,δ′,δ,andβseparately followed by in vitro reconstitution of the polⅢcore and clamp *** we propose a new method for expressing and purifying DNA polymeraseⅢcomponents by utilizing a protein coexpression *** results show that the subunits of the polⅢcore and those of the clamp loader can be coexpressed and purified based on inherent interactions between the *** resulting polⅢcore,clamp loader and sliding clamp can be reconstituted effectively to perform DNA *** strategy considerably simplifies the expression and purification of DNA polymeraseⅢand provides a feasible and convenient method for exploring other multi-subunit systems.

关键词： E.coli DNA polymeraseⅢ coexpression purification

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Design and biosynthesis of functional protein nanostructures

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Science China(life Sciences) 2020年第8期63卷 1142-1158页

作者： Feng li Dianbing Wang Juan Zhou Dong Men xian-en Zhang State Key Laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China

Proteins are one of the major classes of biomolecules that execute biological functions for maintenance of *** kinds of nanostructures self-assembled from proteins have been created in nature over millions of years of evolution,including protein nanowires,layers and *** protein nanostructures can be reconstructed and equipped with desired new *** from and manipulating the self-assembly of protein nanostructures not only help to deepen our understanding of the nature of life but also offer new routes to fabricate novel nanomaterials for diverse *** review summarizes the recent research progress in this field,focusing on the characteristics,functionalization strategies,and applications of protein nanostructures.

关键词： protein nanostructures protein nanowires S-layer protein nanocages self-assembly biosynthesis functionalization

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幽门螺杆菌与反流性食管炎的相关性研究

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胃肠病学和肝病学杂志 2000年第4期9卷 276-278页

作者：余贤恩李莉韦代林赵爱香杜洁贞广西百色地区人民医院内科 533000

目的　探索幽门螺杆菌 (Hp)与反流性食管炎的相关性。方法　对确诊的 43例反流性食管炎及同期条件相匹配的5 0例慢性胃炎及 47例十二指肠溃疡患者采用日产PQ2 0型 0Lympus纤维胃镜检查并取胃粘膜四块用Hp检测试纸行快速尿素酶测定 ,同... 详细信息

目的　探索幽门螺杆菌 (Hp)与反流性食管炎的相关性。方法　对确诊的 43例反流性食管炎及同期条件相匹配的5 0例慢性胃炎及 47例十二指肠溃疡患者采用日产PQ2 0型 0Lympus纤维胃镜检查并取胃粘膜四块用Hp检测试纸行快速尿素酶测定 ,同时取空腹静脉血用EliSA方法测定血Hp -IgG抗体。Hp阴性的反流性食管炎患者 (15例 )分为A、B两组 :A组 (n =7)接受法莫替丁 (2 0mg ,Bid)治疗 ,B组 (n =8)接受法莫替丁 (2 0mg ,Bid)及西沙比利 (10mg ,Tid) ,各治疗 2 8天。Hp阳性反流性食管炎患者 (2 8例 )分为C、D两组 :C组 (n =14 )接受洛赛克 (2 0mg ,Bid)及西沙比利 (10mg ,Tid)治疗 ,D组 (n =14 )接受洛赛克 (2 0mg ,Bid)、羟氨苄青霉素 (0 5 ,Tid)、呋喃唑酮 (0 2 ,Tid)及西沙比利 (10mg ,Tid)治疗 ,各治疗 14天。结果　 1、Hp在反流性食管炎、慢性胃炎、十二指肠溃疡的检出率分别为 65 1%、64 %及 80 8% (P >0 .0 5 )。 2、Ⅰ、Ⅱ、Ⅲ及Ⅳ级食管炎的百分率分别为 3 0 2 %、46 5 %、18 6%及 4 7%。无Ⅴ级食管炎。各级食管炎患者Hp检出率分别为 61 5 %、5 5 %、87 5 %及 10 0 % (P >0 .0 5 )。 3、A、B组治疗显效率 (反流症状消失 )为5 7 1%及 10 0 % (P

关键词：幽门螺杆菌反流性食管炎病理治疗

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