Chromosome-level genome assembly of Salvia miltiorrhiza with orange roots uncovers the role of Sm2OGD3 in catalyzing 15,16-dehydrogenation of tanshinones
作者机构：Key Lab of Chinese Medicine Resources ConservationState Administration of Traditional Chinese Medicine of the People’s Republic of ChinaInstitute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China Engineering Research Center of Chinese Medicine ResourceMinistry of EducationBeijing 100193China
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2023年第10卷第6期
页 面：30-42页
核心收录：
学科分类：0710[理学-生物学] 1008[医学-中药学(可授医学、理学学位)] 0902[农学-园艺学] 10[医学]
基 金：We are thankful for financial support from the CAMS Innovation Fund for Medical Sciences(CIFMS)(2021-I2M-1-029) the National Natural Science Foundation of China(81773836)
摘 要：Salvia miltiorrhiza is well known for its clinical practice in treating heart and cardiovascular *** roots,used for traditional Chinese medicine materials,are usually brick-red due to accumulation of red pigments,such as tanshinone IIA and tanshinone *** we report a *** line(shh)with orange *** with the red roots of normal *** plants,the contents of tanshinones with a single bond at C-15,16 were increased,whereas those with a double bond at C-15,16 were significantly decreased in *** assembled a high-quality chromosome-level genome of *** analysis showed that the relationship between two *** lines with red roots was closer than the relationship with *** indicates that shh could not be the mutant of an extant *** line with red *** genomic and transcriptomic analyses showed that a 1.0 kb DNA fragment was deleted in shh *** assay showed that overexpression of intact Sm2OGD3 in shh hairy roots recovered furan D-ring tanshinone ***,in vitro protein assay showed that Sm2OGD3 catalyzed the conversion of cyptotanshinone,15,16-dihydrotanshinone I and 1,2,15,16-tetrahydrotanshinone I into tanshinone IIA,tanshinone I and 1,2-dihydrotanshinone I,***,Sm2OGD3 functions as tanshinone 15,16-dehydrogenase and is a key enzyme in tanshinone *** results provide novel insights into the metabolic network of medicinally important tanshinone compounds.
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