Temporal transcriptomics reveal the molecular mechanism of dormancy and germination regulated by temperature in Paris polyphylla seed

作     者：Dengqun Liao Yalan Chen Jianjun Qi Hongliang Zhang Peng Sun Caixia Chen Xian'en Li Dengqun Liao;Yalan Chen;Jianjun Qi;Hongliang Zhang;Peng Sun;Caixia Chen;Xian'en Li

作者机构：Institute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100193China Key Lab of Crop Heterosis and Utilization of Ministry of EducationBeijing Key Lab of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100193China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2023年第9卷第4期

页      面：848-866页

核心收录：

学科分类：090706[农学-园林植物与观赏园艺] 0907[农学-林学] 09[农学] 

基　　金：supported by the CAMS Innovation Fund for Medical Sciences (CIFMS) (2017-I2M-3-013) the National Key Research and Development Program of China (Grant No. 2017YFC1700706) the National Natural Science Foundation of China (Grant No. 31471575) 

主　　题：Paris polyphylla var.chinensis Morphophysiologically dormant seed Temperature stratification Time transcriptome Phytohormone Transcription factor Cell wall remodeling gene 

摘      要：The mature seed of Paris polyphylla ***(PPC)is morphophysiologically dormant and develops differently under warm and cold *** elucidate the molecular mechanisms underlying temperature-dependent regulation of PPC seed dormancy and germination,we investigated the dynamic changes in PPC seed transcript levels under warm and cold temperature stratifications(WS and CS,respectively)by time-resolved RNA sequencing,focusing on genes related to hormone metabolism and signaling and cell wall remodeling(CWRM)and encoding transcription factors/regulators(TFs/TRs).A total of 48765 and 47836 differentially expressed genes(DEGs)were associated with WS and CS,*** these,17581 and 16652 DEGs,respectively,unique to WS and CS,and 5386 were common to both temperature stratifications across nine analyzed growth *** expression of hormone metabolism and signaling,TF/TR,and CWRM genes were closely associated with *** genes related to gibberellin(GA),cytokinin,auxin,and brassinosteroid biosynthetic were upregulated in WS as compared to CS seeds,while genes related to dormancy release and germination were downregulated in WS ***,only GA and abscisic acid levels were altered in PPC seeds breaking morphophysiological dormancy(MPD).Overall,37 TF and five TR families were upregulated whereas 24 TF and 16 TR families were downregulated in WS as compared to CS *** CWRM families were highly expressed under WS as compared to CS,suggesting that they promote endosperm weakening and embryo growth of WS seeds and facilitate MPD release and germination.A coexpression analysis revealed positive correlations between TFs/TRs and DEGs involved in hormone metabolism and signaling and *** results provided a basis for investigating the interaction between the endosperm and underdeveloped embryo in the regulation of PPC seed germination and seedling emergence.