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Corrosion resistance of composite coating on magnesium alloy using combined microarc oxidation and inorganic sealing

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中国有色金属学会会刊：英文版 2012年第S3期22卷 760-763页

作者： YANG Wei wang ai-ying JIANG Bai-ling Ningbo Institute of Materials Technology and Engineering Chinese Academy of Sciences School of Material Science and Engineering Xi'an University of Technology

The combined microarc oxidation (MAO) and inorganic sealing process was used to deposit a composite coating to improve the corrosion resistance of AZ31 magnesium *** surface morphologies of the resulting duplex coatings were studied by ***,the corrosion resistance of the coated Mg alloy substrates was investigated using electrochemical workstation and dropping corrosion *** results show that the composite coating surface consists of Mg,Si,O and *** is difficult to deposit inorganic coating on a thick MAO coating *** the composite coating was solidified by CO2 under 175 °C,it exhibits a better corrosion resistance than the MAO monolayer,owing to the thick and compact inorganic coating.

关键词： AZ31 magnesium alloy microarc oxidation composite coating microstructure corrosion resistance

Effect of substrate bias on microstructure and tribological performance of GLC films using hybrid HIPIMS technique

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中国有色金属学会会刊：英文版 2012年第S3期22卷 740-744页

作者： ZHANG Xue-qian KE Pei-ling wang ai-ying HUANG Mei-dong Kwang Ho KIM Ningbo Key Laboratory of Marine Protection Materials Ningbo Institute of Materials Technology and EngineeringChinese Academy of Sciences College of Physics and Electronic Information Tianjin Normal University National Core Research Center for Hybrid Materials Solution Pusan National University

The Cr-doped hydrogen-GLC films were prepared by a hybrid magnetron sputtering system composed of a direct current magnetron sputtering (DC-MS) source of carbon and a high power impulse magnetron sputtering (HIPIMS) source of Cr with reactive gas of *** hydrogen-free GLC and Cr-doped GLC films were also prepared for *** influence of substrate bias on the Cr-doped hydrogen-GLC films was *** the increase of substrate bias from 100 V to 250 V,the re-sputtering of weak bonding sp2 firstly occurred and induced an increased sp3 ***,the following sp3 to sp2 transformation resulted in a decreased sp3 *** change trends of surface roughness and friction coefficient with the increased bias voltages were the same as those of sp3 *** lowest surface roughness and lowest friction coefficient corresponded to the highest sp3 with the Cr-GLC-H films at the bias voltage of-100 V.

关键词： graphite-like carbon high power impulse magnetron sputtering substrate bias microstructure tribological behavior

Transcriptional search to identify and assess reference genes for expression analysis in Solanum lycopersicum under stress and hormone treatment conditions

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Journal of Integrative Agriculture 2022年第11期21卷 3216-3229页

作者： DUAN Yao-ke HAN Rong SU Yan wang ai-ying LI Shuang SUN Hao GONG Hai-jun Shaanxi Engineering Research Center for Vegetables/College of Horticulture Northwest A&F UniversityYangling 712100ShaanxiP.R.China Henan Key Laboratory of Ion-Beam Bioengineering College of Agricultural SciencesZhengzhou UniversityZhengzhou 450000HenanP.R.China

Tomato(Solanum lycopersicum)is a model plant for research on fruit development and stress response,in which gene expression analysis is frequently *** PCR(qPCR)is a widely used technique for gene expression analysis,and the selection of reference genes may affect the accuracy of results and even *** there have been some frequently used reference genes in tomato,it has been shown that the expressions of some of these genes are not constant in different tissues and environmental ***,little information on genomic identification of reference genes is available in ***,we mined the publicly available transcriptional sequencing data and screened out fifteen candidate reference genes,and the expression stability of these candidate genes and seven traditionally used ones were evaluated under stress and hormone *** results showed that over half of the selected candidate references were housekeeping genes in tomato *** the candidate reference genes and the traditionally used ones,the most stably expressed genes varied under different treatments,and most of these genes were recommended as preferred reference genes at least once except Solyc04g009030 and Solyc07g066610,two traditionally used reference *** study provides some novel reference genes in tomato,and the preferred reference genes under different environmental stimuli,which may be useful for future *** study suggests that excavating stably expressed genes from transcriptome sequencing data is a reliable approach to screening reference genes for qPCR analysis.

关键词： tomato(Solanum lycopersicum) gene expression qPCR reference gene expression stability

Cloning and Characterization of karmoisin Homologue Gene(Nlka) in Two Brown Planthopper Strains with Different Eye Colors

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Rice science 2016年第2期23卷 104-110页

作者： LIU Shu-hua TANG Jian LUO Ju YANG Bao-jun wang ai-ying WU Jin-cai School of Plant Protection Yangzhou University State Key Laboratory of Rice Biology China National Rice Research Institute

The brown planthopper （BPH）, Nilaparvata lugens, is a destructive insect pest of rice throughout Asia. Different from brown-eye color wild type, BPH also has red-eye color mutation phenotype. As a visible genetic marker, the red-eye mutant in BPH is a valuable material. To reveal the eye color mutation mechanism, a karmoisin homologue gene （named as Nlka） was cloned from BPH. And karmoisin is always deemd as a xanthommatin-related gene in other insects, encoding phenoxazinone synthetase （PHS）. Nlka is consisted of 7 exons and encodes a protein with 502 amino acids （NIKA）. NIKA showed high amino acid identities with its insect homologues （48.8%-51.8%）. Nlka transcripts can be detected at all the developmental stages and in all tissues tested, including egg, nymph, adult, body wall, ovary, fat body, midgut and Malpighian tubule. However, no constant In/Del or non-synonymous mutation was observed between the mutant and the wild type strains. Quantitative real-time PCR experiment also showed that Nlka transcript level had no significant differences between them. These results indicated that Nlka is not the target gene causing the red-eye color mutation phenotype of BPH. Through the second structure and motif analysis, the present study also showed that all the proteins deduced from the karmoisin genes in insects may be members of monocarboxylate transporters （MCTs） rather than PHSs.

关键词： Nilaparvata lugens red-eye mutant karmoisin monocarboxylate transporter phenoxazinone synthetase rice gene clone

精细化护理联合延续性护理对老年慢性心力衰竭患者遵医行为及生活质量的影响

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心血管康复医学杂志 2024年第2期33卷 127-130页

作者：赵静敏张海平姚艳敏王希柱王艾英唐山市人民医院心内科河北唐山063000 唐山市第二医院心内科唐山市第三医院心内科

目的:研究精细化护理联合延续性护理对老年慢性心力衰竭(CHF)患者遵医行为和生活质量的影响。方法:选择2019年2月至2021年2月我院心内科收治的120例老年CHF患者,根据随机数字表法均分为对照组(给予常规护理干预)和研究组(给予精细化护... 详细信息

目的:研究精细化护理联合延续性护理对老年慢性心力衰竭(CHF)患者遵医行为和生活质量的影响。方法:选择2019年2月至2021年2月我院心内科收治的120例老年CHF患者,根据随机数字表法均分为对照组(给予常规护理干预)和研究组(给予精细化护理联合延续性护理),两组均干预2个月。比较两组干预前后心理状态评估量表(MSSNS)、简易精神状态检查量表(MMSE)、遵医行为调查表、明尼苏达心力衰竭患者生活质量问卷(MLHFQ)评分。结果:干预后,与对照组比较,研究组MSSNS量表评分[(61.02±2.97)分比(55.81±3.38)分]和MLHFQ量表评分[(58.90±3.49)分比(41.70±2.80)分]显著降低,MMSE量表评分[(25.61±3.32)分比(29.68±3.51)分]显著增加,同时研究组的遵医定期复诊、遵医适量康复运动、遵医用药、遵医饮食、遵医稳定情绪百分比均显著增加(P<0.05或<0.01)。结论:精细化护理联合延续性护理可以显著改善老年慢性心力衰竭患者治疗过程中的心理精神状态、遵医行为和生活质量。

关键词：心力衰竭护理病人依从生活质量

Contribution of an Auxin to the Uptake of Nickel and Cadmium in Maize Seedlings

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Biomedical and Environmental Sciences 1999年第3期12卷 170-176页

作者： GUO DONG-SHENG XI Yv-YINC wang ai-ying ZHANG JUN AND YUAN XIAO-ying(Department of Environmentel Science, Shanxi University, Taiyuan, China Analyzingand Detecting Center, Shanxi University, Taiyuan, China) Department of Environmentel Science Shanxi University Taiyuan China

Maize seedlings were cultured in nickel or cadmium contaminated sand treated with α-naphthylacetic acid (NAA). The effeets of NAA on nickel and cadmium uptake in roots, shoots, and subcellular fractions (cell wall, nuclei and remained parts of seedling cells) were determined. The data showed growth promotion when NAA was applied at low concentrations and inhibition at high concentrations. Uptake of nickel and cadmium content increased concurmntly in roots and shoots. In thesubcellular fraction, nickel and cadmium was greatest in the cell wall. The changes in growth had greatest cormlation Wtih nickel and cadmium content in the subeellular fraction.

关键词： Ni Contribution of an Auxin to the Uptake of Nickel and Cadmium in Maize Seedlings

Interactions between myoblasts and macrophages under high glucose milieus result in inflammatory response and impaired insulin sensitivity

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World Journal of Diabetes 2024年第7期15卷 1589-1602页

作者： Wei Luo Yue Zhou Li-ying wang Lei ai Department of Sports and Health Sciences Nanjing Sport InstituteNanjing 210014Jiangsu ProvinceChina Department of Exercise Physiology Beijing Sport UniversityBeijing 100084China Department of Sports Physiology Research Jiangsu Research Institute of Sports ScienceNanjing 210033Jiangsu ProvinceChina

BACKGROUND Skeletal muscle handles about 80% of insulin-stimulated glucose uptake and become the major organ occurring insulin resistance(IR).Many studies have confirmed the interactions between macrophages and skeletal muscle regulated the inflammation and regeneration of skeletal ***,despite of the decades of research,whether macrophages infiltration and polarization in skeletal muscle under high glucose(HG)milieus results in the development of IR is yet to be elucidated.C2C12 myoblasts are well-established and excellent model to study myogenic regulation and its responses to *** exploration of macrophages'role in myoblasts IR and the dynamics of their infiltration and polarization is *** To evaluate interactions between myoblasts and macrophages under HG,and its effects on inflammation and IR in skeletal *** We detected the polarization status of macrophages infiltrated to skeletal muscles of IR mice by hematoxylin and eosin and immunohistochemical ***,we developed an in vitro co-culture system to study the interactions between myoblasts and macrophages under HG *** effects of myoblasts on macrophages were explored through morphological observation,CCK-8 assay,Flow Cytometry,and enzyme-linked immunosorbent *** mediation of macrophages to myogenesis and insulin sensitivity were detected by morphological observation,CCK-8 assay,Immunofluorescence,and 2-NBDG *** The F4/80 and co-localization of F4/80 and CD86 increased,and the myofiber size decreased in IR group(P0.8).In McM+HG group,F4/80+CD86+CD206-cells,monocyte chemoattractant protein 1,TNFα,IL-1βand IL-6 were increased,and F4/80+CD206+CD86-cells and IL-10 were decreased compared with Mc+HG group and McM group(P0.8).Compered to M group,myotube area,myotube n

关键词： Macrophages phenotype Myoblasts Cross-talk Glucose toxicity Chronic inflammation Insulin sensitivity

Rapid and Sensitive Biosensing of Salmonella Using Mechanical Step Rotation and Gold@platinum Nanozymatic Amplification

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Journal of Analysis and Testing 2024年第3期8卷 262-269页

作者： Meixuan Li Lei wang ai Xu ying Ding Fengzhen Yang Yanbin Li Jianhan Lin Key Laboratory of Agricultural Information Acquisition Technology Ministry of Agriculture and Rural AffairsChina Agricultural University Center of Excellence for Poultry Science University of Arkansas

Inspired by ballpoint pen, an optical biosensor is developed for detection of Salmonellausing a mechanical step rotator with a dropper to greatly simplify operation, a high gradient magnetic field with immune magnetic nanobeads to specifically separate bacteria, and immune gold@platinum nanozymes with a smartphone to sensitively detect bacteria. First, the preloaded bacterial sample is drawn by the dropper to react with the immune magnetic nanobeads through repeated squeezing and releasing of the dropper, and the rotator is successively rotated and the dropper is repeatedly squeezed and released to achieve mixing of the bacteria, magnetic nanobeads and gold@platinum nanozymes, followed by magnetic separation to remove the background. Then, the conjugates are eff ectively washed with skim milk and ultrapure water, and the substrate are rapidly catalyzed into the product. Finally, the blue product is photographed and analyzed using a smartphone to obtain the Salmonella concentration. This biosensor could detect Salmonellaas low as 56 CFU/mL in～25 min.

关键词： gold@platinum nanozyme magnetic grid separation mechanical step rotator optical biosensor Salmonella detection

Identification of C-geranylated flavonoids from Paulownia catalpifolia Gong Tong fruits by HPLC-DAD-ESI-MS/MS and their anti-aging effects on 2BS cells induced by H2O2

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Chinese Journal of Natural Medicines 2017年第5期15卷 384-391页

作者： TANG Wen-Zhao wang ying-ai GAO Tian-Yang wang Xiao-Jing ZHAO Yun-Xue Institute of Materia Medica Shandong Academy of Medical SciencesKey Laboratory for Biotech-Drugs Ministry of HealthKey Laboratory for Rare&Uncommon Diseases of Shandong ProvinceJinan 250062China State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China Department of Pharmacology School of MedicineShandong UniversityJinan 250012China

The fruits of Paulownia catalpifolia Gong Tong are used as a Chinese folk herbal medicine for the treatment of enteritis, tonsillitis, bronchitis, and dysentery, etc. Our previous study has identified new C-geranylated flavanones with obvious anti-proliferative effects in lung cancer A549 cells. In the present study, a new C-geranylated flavone, paucatalinone C(1) and five known C-geranylated flavanones(2-6) were isolated. In addition, a total of 34 C-geranylated flavonoids were detected by HPLC-DAD-ESI-MS/MS coupling techniques from the CH_2Cl_2 extract of P. catalpifolia. Futhermore, anti-aging effects of isolated compounds were evaluated in vitro with premature senescent 2BS cells induced by H_2O_2. Phytochemical results indicated that P. catalpifolia was a natural resource of abundant C-geranylated flavonoids. Diplacone(3) and paucatalinone A(5) were the potent anti-aging agents in the premature senescent 2BS cells induced by H_2O_2 and the C-geranyl substituent may be an important factor because of its lipophilic character.

关键词： Paulownia catalpifolia Gong Tong C-geranylated flavonoids Paucatalinone C HPLC-DAD-ESI-MS/MS Anti-aging effects 2BS cells