Rapid and Sensitive Biosensing of Salmonella Using Mechanical Step Rotation and Gold@platinum Nanozymatic Amplification

作     者：Meixuan Li Lei Wang Ai Xu Ying Ding Fengzhen Yang Yanbin Li Jianhan Lin 

作者机构：Key Laboratory of Agricultural Information Acquisition TechnologyMinistry of Agriculture and Rural AffairsChina Agricultural UniversityBeijing100083China Center of Excellence for Poultry ScienceUniversity of ArkansasFayettevilleAR72701USA 

出 版 物：《Journal of Analysis and Testing》 (分析检测（英文）)

年 卷 期：2024年第8卷第3期

页      面：262-269页

核心收录：

学科分类：080202[工学-机械电子工程] 08[工学] 09[农学] 0903[农学-农业资源与环境] 0802[工学-机械工程] 

基　　金：supported by Pinduoduo-China Agricultural University Research Fund(PC2023B02021) 

主　　题：optical biosensor mechanical step rotator gold@platinum nanozyme magnetic grid separation Salmonella detection 

摘      要：Inspired by ballpoint pen,an optical biosensor is developed for detection of Salmonella using a mechanical step rotator with a dropper to greatly simplify operation,a high gradient magnetic field with immune magnetic nanobeads to specifically separate bacteria,and immune gold@platinum nanozymes with a smartphone to sensitively detect ***,the preloaded bacterial sample is drawn by the dropper to react with the immune magnetic nanobeads through repeated squeezing and releasing of the dropper,and the rotator is successively rotated and the dropper is repeatedly squeezed and released to achieve mixing of the bacteria,magnetic nanobeads and gold@platinum nanozymes,followed by magnetic separation to remove the ***,the conjugates are effectively washed with skim milk and ultrapure water,and the substrate are rapidly catalyzed into the ***,the blue product is photographed and analyzed using a smartphone to obtain the Salmonella *** biosensor could detect Salmonella as low as 56 CFU/mL in~25 min.