Identification of C-geranylated flavonoids from Paulownia catalpifolia Gong Tong fruits by HPLC-DAD-ESI-MS/MS and their anti-aging effects on 2BS cells induced by H2O2

作     者：TANG Wen-Zhao WANG Ying-Ai GAO Tian-Yang WANG Xiao-Jing ZHAO Yun-Xue 

作者机构：Institute of Materia MedicaShandong Academy of Medical SciencesKey Laboratory for Biotech-Drugs Ministry of HealthKey Laboratory for Rare&Uncommon Diseases of Shandong ProvinceJinan 250062China State Key Laboratory of Bioactive Substance and Function of Natural MedicinesInstitute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China Department of PharmacologySchool of MedicineShandong UniversityJinan 250012China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2017年第15卷第5期

页      面：384-391页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100704[医学-药物分析学] 10[医学] 

基　　金：financially supported by the Natural Science Foundation of Shandong Academy of Medical Sciences(No.2015-08) the Innovation Project of Shandong Academy of Medical Sciences and the Natural Science Open Foundation of State Key Laboratory of Bioactive Substance and Function of Natural Medicines(No.GTZK201503) 

主　　题：Paulownia catalpifolia Gong Tong C-geranylated flavonoids Paucatalinone C HPLC-DAD-ESI-MS/MS Anti-aging effects 2BS cells 

摘      要：The fruits of Paulownia catalpifolia Gong Tong are used as a Chinese folk herbal medicine for the treatment of enteritis, tonsillitis, bronchitis, and dysentery, etc. Our previous study has identified new C-geranylated flavanones with obvious anti-proliferative effects in lung cancer A549 cells. In the present study, a new C-geranylated flavone, paucatalinone C(1) and five known C-geranylated flavanones(2-6) were isolated. In addition, a total of 34 C-geranylated flavonoids were detected by HPLC-DAD-ESI-MS/MS coupling techniques from the CH_2Cl_2 extract of P. catalpifolia. Futhermore, anti-aging effects of isolated compounds were evaluated in vitro with premature senescent 2BS cells induced by H_2O_2. Phytochemical results indicated that P. catalpifolia was a natural resource of abundant C-geranylated flavonoids. Diplacone(3) and paucatalinone A(5) were the potent anti-aging agents in the premature senescent 2BS cells induced by H_2O_2 and the C-geranyl substituent may be an important factor because of its lipophilic character.