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Chlorella Residue Functions as a Bio-Stimulant to Promote Plant Growth and Improve Soil Fertility

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Journal of Agricultural Chemistry and Environment 2024年第4期13卷 373-383页

作者： Asuka Okazaki Toru Mizoguchi Zakirul Islam quoc Thinh tran Motoki Kubo Laboratory of Bioengineering Department of Biotechnology Graduate School of Advanced Life Sciences Ritsumeikan University Shiga Japan Production and Development Department Sun Chlorella Co. Ltd. Kyoto Japan

Chlorella residues are currently underutilized. Therefore, in this study, we analyzed the nutritional components of Chlorella residue, and investigated its potential use as an organic fertilizer/bio-stimulant. Composition analyses revealed that the Chlorella residue contained a substantial amount of nitrogen (97,910 mg/kg), and significant quantities of secondary macronutrients, such as calcium (4300 mg/kg) and magnesium (9700 mg/kg), and micronutrients, such as iron (1850 mg/L) and manganese (359 mg/kg). The application of Chlorella residue to soil resulted in increased soil bacterial biomass. When Chlorella residue was added to the soil at a rate of 0.5% or 1.0% (w/w), the fresh weights of Brassica rapa and Spinacia oleracea were significantly increased. Furthermore, the application of Chlorella residue to the soil of B. rapa suppressed the reduction of the microbiome caused by clubroot disease and decreased the clubroot disease index. Therefore, Chlorella residue can be included in organic fertilizers that effectively improve soil nutrient contents, promote plant growth, and reduce the incidence of disease.

关键词： Bacterial Biomass Bacterial Diversity Chlorella Residue Clubroot Disease Plant Disease

Research on Cassava Drying, Application and Promotion of the Reversible Airflow Flatbed Dryers for Sliced Cassava

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Journal of Food Science and Engineering 2015年第3期5卷 150-158页

作者： tran Van tuan Le Quang Vinh Nguyen Van Xuan Center for Agricultural Energy and Machinery Nong Lam University Ho Chi Minh City 700000 Vietnam

The study was conducted by researchers of the Research Center for Agricultural Energy and Machinery （CAEM） of the Ho Chi Minh City Nong Lam University （NLU） with the objectives of researching on drying ways for cassava and of evaluating the adaptability of the reversible-airflow dryers in drying of cassava. The results obtained were as follows： done with thin-layer drying experiments, deep-layer drying experiments in the lab, and conducted drying experiments at the cassava dryer in actual production scale with two capacity sizes of 8 tons/batch and 16 tons/batch, that operates based on principle of reversible-airflow drying （SRA dryers）. By the means of drying experiments, the performance of the SRA dryers such as optimal drying temperature, drying time, drying airflow reversal timing, moisture content uniformity of the sliced cassava mass after finishing drying process, drying cost, etc. was defined, the optimal drying temperature was 70 ~C, the total actual drying time for finishing one batch was 25-28 hours, applicable time for drying airflow reversal was after drying 16 hours since startup, the deviation in moisture content of the cassava mass after drying was just 2%-3%, the drying costs （per one kg of dried cassava） calculated were in turns VND407/kg at the SRA-8 dryer （8 tons/batch）, and VND351/kg at the SRA-16 dryer （16 tons/batch）. Obviously, comparing with the sale price of dry cassava at present, these levels of drying cost are accounting for 7%-10%, which are suitable and acceptable. Thus, investment of the dryers for sliced cassava would be effective and contribute to bring more income for Vietnamese cassava farmers and dryer end-users.

关键词： Cassava sliced cassava SRA dryer wood-chip furnace.

Polyelectrolyte complex micelles by self-assembly of polypeptide-based triblock copolymer for doxorubicin delivery

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Asian Journal of Pharmaceutical Sciences 2014年第4期9卷 191-198页

作者： Jeong Hwan Kim Thiruganesh Ramasamy tuan Hiep tran Ju Yeon Choi Hyuk Jun Cho Chul Soon Yong Jong Oh Kim College of Pharmacy Yeungnam University214-1Dae-DongGyongsan 712-749South Korea

Polyelectrolyte complex micelles were prepared by self-assembly of polypeptide-based triblock copolymer as a new drug carrier for cancer *** triblock copolymer,poly(L-aspartic acid)-b-poly(ethylene glycol)-b-poly(L-aspartic acid)(PLD-b-PEG-b-PLD),spontaneously self-assembled with doxorubicin(DOX)via electrostatic interactions to form spherical micelles with a particle size of 60e80 nm(triblock ionomer complexes micelles,TBIC micelles).These micelles exhibited a high loading capacity of 70%(w/w)at a drug/polymer ratio of 0.5 at pH *** showed pH-responsive release patterns,with higher release at acidic pH than at physiological ***,DOX-loaded TBIC micelles exerted less cytotoxicity than free DOX in the A-549 human lung cancer cell *** microscopy in A-549 cells indicated that DOX-loaded TBIC micelles were transported into lysosomes via *** micelles possessed favorable pharmacokinetic characteristics and showed sustained DOX release in ***,these findings indicate that PLDb-PEG-b-PLD polypeptide micelles are a promising approach for anti-cancer drug delivery.

关键词： Polyelectrolyte Micelles Drug delivery Poly(L-aspartic acid) Poly(ethylene glycol)

rAL Effectors Drive transcription Bidirectionally in Plants

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Molecular Plant 2017年第2期10卷 285-296页

作者： Li Wang Fabio C. Rinaldi Pallavi Singh Erin L. Doyle Zoe E. Dubrow tuan Tu tran Alvaro L. Perez-Quintero Boris Szurek Adam J. Bogdanove Plant Pathology and Plant-Microbe Biology Section School of Integrative Plant Science Cornell University 334 Plant Science Building Ithaca NY 14853 USA Department of Biology Doane University 1014 Boswell Avenue Crete NE 68333 USA UMR Interactions-Plantes-Microorganismes-Environnement IRD-Cirad-Universite Montpellier Montpellier France

TAL effectors delivered by phytopathogenic Xanthomonas species are DNA-sequence-specific transcrip- tional activators of host susceptibility genes and sometimes resistance genes. The modularity of DNA recognition by TAL effectors makes them important also as tools for gene targeting and genome editing. Effector binding elements （EBEs） recognized by native TAL effectors in plants have been identified only on the forward strand of target promoters. Here, we demonstrate that TAL effectors can drive plant tran- scription from EBEs on either strand and in both directions. Furthermore, we show that a native TAL effector from Xanthomonas oryzae pv. oryzicola drives expression of a target with an EBE on each strand of its promoter. By inserting that promoter and derivatives between two reporter genes oriented head to head, we show that the TAL effector drives expression from either EBE in the respective orientations, and that activity at the reverse-strand EBE also potentiates forward transcription driven by activity at the forward-strand EBE. Our results reveal new modes of action for TAL effectors, suggesting the possibility of yet unrecognized targets important in plant disease, expanding the search space for off-targets of custom TAL effectors, and highlighting the potential of TAL effectors for probing fundamental aspects of plant transcription.

关键词： xanthomonas transcription activator-like effector bidirectional reporter enhancer element off-target

20-Hydroxyecdysone from Dacrycarpus imbricatus bark inhibits the proliferation of acute myeloid leukemia cells

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Asian Pacific Journal of Tropical Medicine 2017年第2期10卷 151-153页

作者： Trinh Thi Thuy Nguyen Thanh Tam Nguyen Thi Hoang Anh Dang Viet Hau Dinh Thi Phong Le quoc Thang Sabrina Adorisio tran Van Sung Domenico V Delfino Institute of Chemistry Vietnam Academy of Science and Technology Vietnam National Museum of Nature Vietnam Academy of Science and Technology Hue University’s College of Educations Section of Pharmacology Department of MedicineUniversity of Perugia

Objective:To investigate the anti-proliferative effects of 20-hydroxyecdysone isolated from the bark of Dacrycarpus imbricatus(Blume) de ***:Column chromatography was used for isolation of compounds from plant *** structure of the isolated compound was identified by mass spectrometry and nuclear magnetic resonance techniques,including HSQC,HMBC,NOE-difference *** isolated compound was tested for its anti-proliferative activity in acute myeloid leukemia(AML) and OCI-AML ***:Compound 1 was isolated from the ethyl acetate fraction of Dacrycarpus imbricatus barks by column *** chemical structure was identified as 20-hydroxyecdysone(20HE),a cholestane-type ecdysteroid,by a combination of mass spectrometry and nuclear magnetic resonance spectrometric *** goal was to test the anti-proliferative activity of 20 HE using the OCI-AML cell line.20 HE significantly decreased OCI cell number at a concentration of 1 mg/mL,whereas lower concentrations were ***,this decrease was due to partial blockage of the G_1/S phase of the cell cycle,with a reduction of cells in the G_2M phase,not due to increased ***:This indicates that 20 HE significantly decreases the number of cells in the G_1/S phase of the cell cycle in human AML *** is the first time that the anti-proliferative activity of 20 HE against a human tumor cell line has been reported.

关键词： Dacrycarpus imbricatus Ecdysteroid 20-hydroxyecdysone Proliferation Acute myeloid leukemia

Necrotizing Scleritis Associated with 5-Fluorouracil and Sub-Tenon’s Block in Patient with Previous Trabeculectomy: A Case Report

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Open Journal of Ophthalmology 2013年第3期3卷 73-75页

作者： tuan A. tran Jeremy O’Connor Xavier Fagan Tu tran Dan Nguyen Department of Glaucoma Victorian Eye and Ear Hospital Melbourne Australia Department of Medical Ophthalmology Victorian Eye and Ear Hospital Melbourne Australia. Department of Politecnico Ophthalmology Sydney Eye Hospital Sydney Australia

Necrotizing scleritis is the most severe form of scleritis with a significant threat to vision and globe integrity. It can be infectious, surgically induced necrotizing scleritis (SINS) or systemic autoimmune associated. We report a case of necrotizing scleritis associated with 5-fluorouracil (5-FU) at the site of sub tenon’s block in a patient with previous trabeculectomy. To our knowledge, this is the first reported case of necrotizing scleritis associated with 5-FU. This may implicate alternative approaches to local anaesthetic techniques when using adjunctive 5-FU.

关键词： Necrotizing Scleritis 5-Fluorouracil Sub-Tenon’s Block Glaucoma

Interventions of side branches in the management of left ventricular dysfunction： a study based on the virtual dual chamber of the left ventricle

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Chinese Medical Journal 2012年第22期125卷 4126-4129页

作者： Thach Nguyen Dan Le James Nguyen Joseph Hackler Katrina Nguyen Hung Phan tuan D Nguyen quoc Nguyen Department of Cardiology Community Healthcare SystemSt Mary Medical CenterHobart IndianaUSA Cardiology Fellow Heart Failure-Transplant programthe University of Alabama in Birmingham(UAB)BirminghamAlabamaUSA Cardiology Fellow the University of Arizona Medical Center TucsonArizonaUSA Undergraduate Program at the University of California at San Diego San DiegoCaliforniaUSA Department of Medicine Weiss Memorial HospitalChicagoIllinoisUSA Staff Research Associate University of California at Los AngelesSchool of Public HealthLos AngelesCAUSA University of Indianapolis IndianapolisIndianaUSA

For interventional cardiologists, it is very challenging to do percutaneous coronary interventions （PCI） in lesions of ihe left main, bifilrcations or of chronic total occlusion types. However, it is more challenging to demonstrate an evidence based rationale supporting the indications of PCI in these lesions. In this article, new questions are raised on the benefits and the mechanism of benefits for PCI of bifilrcations revolving large side branches such as the diagonal （DIAG）,

关键词： diastolic dysfunction percutaneous coronary interventions, bifurcation lesions

Improved Flame Retardant Properties of Polymers Epoxy Based on Antimony Trioxide/Chlorinated Paraffin

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材料科学与工程（中英文A版） 2014年第5期4卷 146-150页

作者： Bach Trong Phuc tran Vinh Dieu Nguyen tuan Anh Center Polymers and Composite Materials Hanoi University of Science and Technology Hanoi Viet Nam Faculty of Chemical Technology Hanoi University of Industry Minh Khai ward Tu Liem Ha Noi Viet Nam

Molybdate Doped Polypyrrole： Preparation, Properties and Application

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材料科学与工程（中英文A版） 2013年第5期3卷 321-328页

作者： Ha Manh Hung Le Minh Duc tran Vinh Dieu Vu quoc Trung Faculty of Chemistry Hanoi National University of Education Cau Giay District Hanoi Vietnam Faculty of General Education Hanoi University of Mining and Geology Dong Ngac Tu Liem Hanoi Vietnam Faculty of Chemical Engineering Danang University oJ l"echnology Lien Chieu District Danang Vietnam Centre of Polymer Research Hanoi University of Science and Technology Hai Ba Trung District Hanoi Vietnam