rAL Effectors Drive Transcription Bidirectionally in Plants

作     者：Li Wang Fabio C. Rinaldi Pallavi Singh Erin L. Doyle Zoe E. Dubrow Tuan Tu Tran Alvaro L. Perez-Quintero Boris Szurek Adam J. Bogdanove 

作者机构：Plant Pathology and Plant-Microbe Biology Section School of Integrative Plant Science Cornell University 334 Plant Science Building Ithaca NY 14853 USA Department of Biology Doane University 1014 Boswell Avenue Crete NE 68333 USA UMR Interactions-Plantes-Microorganismes-Environnement IRD-Cirad-Universite Montpellier Montpellier France 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2017年第10卷第2期

页      面：285-296页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：supported by the Plant Genome Research Program of the National Science Foundation 美国国立卫生研究院(NIH)项目 

主　　题：xanthomonas transcription activator-like effector bidirectional reporter enhancer element off-target 

摘      要：TAL effectors delivered by phytopathogenic Xanthomonas species are DNA-sequence-specific transcrip- tional activators of host susceptibility genes and sometimes resistance genes. The modularity of DNA recognition by TAL effectors makes them important also as tools for gene targeting and genome editing. Effector binding elements （EBEs） recognized by native TAL effectors in plants have been identified only on the forward strand of target promoters. Here, we demonstrate that TAL effectors can drive plant tran- scription from EBEs on either strand and in both directions. Furthermore, we show that a native TAL effector from Xanthomonas oryzae pv. oryzicola drives expression of a target with an EBE on each strand of its promoter. By inserting that promoter and derivatives between two reporter genes oriented head to head, we show that the TAL effector drives expression from either EBE in the respective orientations, and that activity at the reverse-strand EBE also potentiates forward transcription driven by activity at the forward-strand EBE. Our results reveal new modes of action for TAL effectors, suggesting the possibility of yet unrecognized targets important in plant disease, expanding the search space for off-targets of custom TAL effectors, and highlighting the potential of TAL effectors for probing fundamental aspects of plant transcription.