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科教文汇 2015年第14期 168-169页

作者：夏田禾四川外国语大学英语学院重庆400000

心理活动是影响学生学习效果的内在因素,通过正确引导与激发学习者的心理反应有助于促进外语课堂教学。该文从优化刺激环节、把握环境特点和主体特性、遵循教学一般原则等方面,初步探讨了"心理刺激"手段在外语教学中的有效运用。

关键词：心理刺激环节主体原则

Survivin protects neonatal mouse cardiomyocytes from CVB3-induced apoptosis in a caspase-dependent manner

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Survivin protects neonatal mouse cardiomyocytes from CVB3-in...

硫化氢对柯萨奇病毒B3心肌炎小鼠血红素加氧酶-1/一氧化碳通路的影响

2019年浙江省医学会儿科学分会学术大会

作者： Tingting Wu Ping Li Hao Li Yi Zhan Songyue Zhang Youyang Shi Yaoyao Yan tianhe xia Zhenquan Wang Rongzhou Wu The Second Affiliated Hospital of Wenzhou Medical University

Background Viral myocarditis(VMC) is a kind of inflammatory cardiomyopathy mainly affecting children and young adults and resulting in heart failure due to dilated cardiomyopathy or cardiac arrest. Cardiomyocyte apoptosis has been shown to play a critical role in the pathogenesis of coxsackievirus B3(CVB3) induced VMC and blocking of this process may contribute to the therapeutic effect towards VMC. Therefore, the aim of this study was to examine whether survivin, one of the strongest antiapoptotic proteins, participates in the apoptotic activity after CVB3 infection and further reveal its related *** and results Herethe cultured neonatal mouse cardiomyocytes(NMCs) were exposed to CVB3 to establish the cell model of VMC and we found a time-dependent manner of survivin expression after CVB3 infection by Western Blot. Lentivirus was next used to mediate survivin expression thereby examine the function of survivin in CVB3-infected NMCs. TUNEL assay demonstrated that survivin overexpression interrupted CVB3-induced apoptosis and significantly decreased the apoptotic rate at 36 h and 60 h after CVB3 infection. It was next examined whether caspase-3 and-9 were involved in the antiapoptotic pathway initiated by survivin via Western Blot. The results showed a reverse relationship between survivin expression and cleaved caspase-3 and-9 expression, suggesting that survivin may inhibit apoptosis by restraining the activity of caspase-3 and-9. Moreover, the supernatant from cultured NMCs was extracted to detect the quantitation of released LDH and a sharp decrease was shown in survivin overexpressed group compared to CVB3 infected group, indicating a protective role of survivin in CVB3-induced cell *** In the present study, we demonstrated that survivin was triggered by CVB3 infection in NMCs in a time-dependent manner and executed its antiapoptotic effects via caspase-3-and caspase-9-dependent signaling pathway.

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中华心血管病杂志 2017年第9期45卷 765-769页

作者：张松跃吴婷婷任跃夏天和吴蓉洲温州医科大学附属第二医院育英儿童医院儿童心脏中心温州医科大学心脏发育与转化医学研究所 325027

目的探讨硫化氢（H2S）对柯萨奇病毒B3（CVB3）心肌炎小鼠血红素加氧酶-1（HO-1）/一氧化碳通路的影响.方法清洁级近交系雄性Balb/c小鼠70只,分为正常组（n=10）、心肌炎组（n=20）、炔丙基甘氨酸组（PAG组,n=20）和硫氢化钠组（NaHS... 详细信息

目的探讨硫化氢（H2S）对柯萨奇病毒B3（CVB3）心肌炎小鼠血红素加氧酶-1（HO-1）/一氧化碳通路的影响.方法清洁级近交系雄性Balb/c小鼠70只,分为正常组（n=10）、心肌炎组（n=20）、炔丙基甘氨酸组（PAG组,n=20）和硫氢化钠组（NaHS组,n=20）.心肌炎组、PAG组和NaHS组分别在第0天每只小鼠腹腔注射0.1 ml10-5 69TCID50m·ml-1的CVB3建立心肌炎模型,从第0天起PAG组予以炔丙基甘氨酸（PAG）40 mg·kg-1·d-1腹腔注射,NaHS组予以硫氢化钠（NaHS） 50 μmol·kg-1·d-1腹腔注射,心肌炎组予以等量磷酸盐缓冲液（PBS）腹腔注射.对照组从第0天起每只小鼠每日腹腔注射不含病毒的等量灭菌PBS液.观察至接种后第10天各组取10只小鼠,记录小鼠体重,采血后处死留取心脏标本并记录其质量.采用心脏质量/体重比值衡量心肌水肿程度,以反应心肌炎症程度.苏木素伊红染色观察小鼠心肌病理改变并计算炎症积分.检测各组小鼠血清碳氧血红蛋白（COHb）表达水平.逆转录聚合酶链反应检测心肌细胞中HO-1 mRNA的表达水平.结果（1）各组小鼠心肌病理学特征：正常组小鼠心肌细胞结构正常,心肌纤维排列整齐,胞浆丰富均匀,横纹清晰,间质无水肿.心肌炎组、PAG组和NaHS组小鼠心肌内均可见心肌细胞坏死崩解,周围伴有大量炎症细胞浸润,以单核细胞和淋巴细胞为主,PAG组心肌细胞坏死及炎症浸润更为明显,坏死灶内可见蓝色钙化颗粒,胞核和细胞轮廓消失,NaHS组炎症较轻,心肌炎症细胞浸润范围较心肌炎组和PAG组小.（2）各组小鼠心脏质量/体重比值和心肌炎症积分：心肌炎组、PAG组和NaHS组小鼠心脏质量/体重比值均明显高于正常组（P均＜0.05）,其中PAG组又明显高于心肌炎组（P＜0.05）,而NaHS组则明显低于心肌炎组（P=0.001）.心肌炎组、PAG组和NaHS组小鼠心肌炎症积分均明显高于正常组（P均＜0.05）,但NaHS组明显低于心肌炎组（P =0.006）,PAG组则明显高于心肌炎组（P =0.038）.（3）各组小鼠血清COHb的表达水平：心肌炎组、PAG组和NaHS组小鼠血清COHb表达水平均明显高于正常组（P均＜0.05）,其中PAG组又明显低于心肌炎组（P=0.000）,NaHS组则明显高于心肌炎组（P =0.000）.（4）各组小鼠心肌HO-1 mRNA的表达水平：心肌炎组、PAG组和NaHS组小鼠心肌HO-1 mRNA的表达水平均明显高于正常组（P均＜0.05）,但PAG组明显低于心肌炎组（P＜0.05）,NaHS组则明显高于心肌炎组（P＜0.05）.（5）小鼠全血COHb表达水平、心肌细胞HO-1 mRNA表达水平及心肌炎症积分三者间的相关性：小鼠全血COHb表达水平与心肌细胞HO-1 mRNA表达水平呈正相关（r=0.927,P=0.000）,心肌炎症积分与全血COHb表达水平呈负相关（r=-0.753,P=0.000）,心肌炎症积分与心肌细胞HO-1 mRNA表达水平呈负相关（r=-0.754,P=0.000）.结论 H2S可诱导CVB3心肌炎小鼠心肌组织HO-1的表达从而上调HO-1/一氧化碳通路,进而发挥心肌保护作用.

关键词：心肌炎硫化氢碳氧血红蛋白血红素加氧酶-1 一氧化碳

Transcatheter Closure of Perimembranous Ventricular Septal Defects Using an Occluder: 5-Year Outcomes

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丹参酮通过调节JAK2/STAT1通路减轻病毒性心肌炎小鼠的心肌损伤

2019年浙江省医学会儿科学分会学术大会

作者： Hao Li Youyang Shi Songyue Zhang Yue Ren Xing Rong Zhenquan Wang tianhe xia Tingting Wu Haitang Xu Yaoyao Yan Yi Zhan Maoping Chu Rongzhou Wu The Second Affiliated Hospital of Wenzhou Medical University

Background Transcatheter closure of perimembranous ventricular septal defects is one of the greatest challenges in interventional cardiology. Long-term follow-up data are limited. This report presents our experience with transcatheter closure of perimembranous ventricular septal defects using an *** 253 patients included in the database of the Second Affiliated Hospital and Yuying Children’s Hospital from January 2011– December 2015 with transcatheter closure of perimembranous ventricular septal defects and discharged from follow-up. All patients were invited for clinical and transthoracic echocardiography, electrocardiogram, and thoracic radiography *** Device implantation was successful in 252 of 253 patients(99.6%). The median age was 42 months(range 27 months–18 years). The patients were followed for 6–60 months. The mean defect diameter was 3.5 ± 1.4 mm and the mean size of the ventricular septal defect rim below the aortic valve was 3.7 ± 1.8 mm. The mean diameter of the devices used was 4 mm. Thirty-seven patients developed arrhythmia after the procedure and recovered within 24 months;four patients had hemolysis and four had moderate tricuspid valve regurgitation. No other serious adverse event occurred during the follow-up *** Transcatheter closure of perimembranous ventricular septal defects using an occluder is safe and effective in most patients. Additional studies with larger cohorts and longer follow-up periods are needed to further evaluate the safety of this method. The use of a smaller occluder may shorten the operation time, decreasing the incidence of postoperative arrhythmia.

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中华心血管病杂志 2015年第2期43卷 167-172页

作者：夏天和吴婷婷邬涛任跃王珍全吴蓉洲温州医科大学附属第二医院育英儿童医院儿童心脏中心 325027

目的观察丹参酮对病毒性心肌炎小鼠心肌是否具有保护作用,并探讨其作用机制.方法清洁级近交系Balb/c小鼠,雄性,4～6周龄,共110只.采用随机数字表法将小鼠分为5组,分别为心肌炎对照组、JAK激酶2（janus kinase 2,JAK2）抑制剂组（JAK2... 详细信息

目的观察丹参酮对病毒性心肌炎小鼠心肌是否具有保护作用,并探讨其作用机制.方法清洁级近交系Balb/c小鼠,雄性,4～6周龄,共110只.采用随机数字表法将小鼠分为5组,分别为心肌炎对照组、JAK激酶2（janus kinase 2,JAK2）抑制剂组（JAK2抑制剂组）、丹参酮ⅡA组（丹参酮组）、JAK2抑制剂加用丹参酮组（JAK2抑制剂＋丹参酮组）各25只,以及正常对照组10只.苏木精伊红（HE）染色后光镜下观察小鼠心肌组织病理学改变.免疫组织化学法、蛋白质印迹分析法检测心肌信号转导子与转录激活子1（STAT1）磷酸化表达水平.化学发光法测定小鼠血清心肌肌钙蛋白-Ⅰ（cTnI）的表达水平.将心肌病理积分与磷酸化STAT1（p-STAT1）的免疫组织化学吸光度值、血清cTnI含量作相关性分析.结果（1）心肌病理炎症积分检测结果：JAK2抑制剂组和JAK2抑制剂＋丹参酮组小鼠心肌病理炎症积分均明显高于心肌炎对照组（3.35±0.57和3.34±0.54比2.12±0.39,P均＜0.01）,丹参酮组小鼠心肌病理炎症积分明显低于心肌炎对照组（1.40 ±0.34比2.12±0.39,P＜0.01）.（2）心肌细胞p-STAT1表达水平的检测结果：JAK2抑制剂组和JAK2抑制剂＋丹参酮组小鼠心肌p-STAT1表达水平的平吸光度值均明显低于心肌炎对照组（0.017±0.010和0.020±0.010比0.246±0.010,P均＜0.01）,与正常对照组比较差异则无统计学意义（P＞0.05）,而丹参酮组心肌p-STAT1表达水平则明显高于心肌炎对照组（0.444±0.060比0.246±0.010,P＜0.01）.（3）血清cTnI水平检测结果：心肌炎对照组、JAK2抑制剂组、丹参酮组及JAK2抑制剂＋丹参酮组小鼠血清cTnI水平均明显高于正常对照组[（0.42±0.06）、（1.17±0.25）、（0.23-±0.05）和（1.04±0.19） μg/L比（0.02±0.01） μg/L,P均＜0.01].JAK2抑制剂组和JAK2抑制剂＋丹参酮组小鼠血清cTnI水平均明显高于心肌炎对照组[（1.17±0.25）和（1.04±0.19） μg/L比（0.42 ±0.06） μg/L,P均＜0.01],丹参酮组小鼠血清cTnI水平则明显低于心肌炎对照组[（0.23±0.05） μg/L比（0.42±0.06） μg/L,P＜0.01].（4）相关性分析结果：p-STAT1的免疫组织化学吸光度值与心肌病理炎症积分呈负相关（R2 =0.738,P＜0.01）,血清cTnI含量与心肌病理炎症积分呈正相关（R2=0.766,P＜0.01）.结论丹参酮可通过活化JAK2-STAT1通路,上调p-STAT1表达,从而减轻病毒性心肌炎小鼠的心肌损伤.

关键词：心肌炎丹参酮

基于低通滤波的离网型风/氢耦合系统容量配置优化

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吉林电力 2022年第4期50卷 31-36页

作者：刘子锐夏天贺李振新东北电力大学吉林吉林132012 中能建路桥工程有限公司天津300203 国网吉林供电公司吉林吉林132000

针对提高风电消纳率和氢能利用率的需求,提出构建以风力发电机组、氢储能系统、蓄电池和负载为核心设备的离网型风/氢耦合系统,使风力发电机组输出功率满足负载需求,将经济成本、失电率和弃风率作为指标,对风电机组、氢储能系统以及蓄... 详细信息

针对提高风电消纳率和氢能利用率的需求,提出构建以风力发电机组、氢储能系统、蓄电池和负载为核心设备的离网型风/氢耦合系统,使风力发电机组输出功率满足负载需求,将经济成本、失电率和弃风率作为指标,对风电机组、氢储能系统以及蓄电池进行最优容量配置,基于粒子群算法求解得到满足经济性和供电可靠性的配置结果,并通过仿真算例验证了配置方案的正确性。

关键词：风/氢耦合容量配置低通滤波粒子群算法

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无代理技术的设计与实现

计算机与数字工程 2019年第8期47卷 1912-1916,1940页

作者：刘清王帆夏天鹤施涛中国西安卫星测控中心

代理的大量使用会导致额外资源消耗增加的问题,同时当今云计算领域无代理技术因多次I/O操作的模拟而存在一定的处理时延。为了解决这些问题,设计了一种高效的无代理技术的实现架构,从减少代理数量和降低处理时延两个方面进行优化改进。... 详细信息

代理的大量使用会导致额外资源消耗增加的问题,同时当今云计算领域无代理技术因多次I/O操作的模拟而存在一定的处理时延。为了解决这些问题,设计了一种高效的无代理技术的实现架构,从减少代理数量和降低处理时延两个方面进行优化改进。研究了用户请求捕获响应、虚拟磁盘多机同时访问、操作隔离和降低处理时延等设计实现难点问题的解决方案。实验表明设计的无代理技术,能够避免传统代理模式导致的额外资源消耗问题;读写操作效率,分别是一般无代理模式的6.3倍和4.1倍,传统代理模式的9.7倍和5.7倍,可以大幅提高处理效率。

关键词：无代理操作映射隔离

温州医科大学附属育英儿童医院2000-2011年住院儿童死亡原因分析

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温州医学院学报 2014年第1期44卷 55-58页

作者：夏天和王珍全张园海谢作楷温州医科大学附属育英儿童医院心内科浙江温州325027 温州医科大学附属育英儿童医院病案室浙江温州325027

目的:了解温州医科大学附属育英儿童医院(以下简称育英儿童医院)住院儿童近12年死亡原因及死亡儿童的性别、年龄分布情况。方法:对2000-2011年儿童死亡病例进行回顾性分析,根据ICD-10国际疾病分类标准,采用SPSS 17.0进行统计学分析。结... 详细信息

目的:了解温州医科大学附属育英儿童医院(以下简称育英儿童医院)住院儿童近12年死亡原因及死亡儿童的性别、年龄分布情况。方法:对2000-2011年儿童死亡病例进行回顾性分析,根据ICD-10国际疾病分类标准,采用SPSS 17.0进行统计学分析。结果:2000-2011年育英儿童医院住院儿童死亡病例共539例,男:女约为2.12:1,农村:城市约为1.72:1。在所有年龄段中,<1岁儿童死亡比例最高。各年龄段死亡原因不同,新生儿死因顺位前3位为:先天畸形、新生儿肺透明膜病、新生儿窒息;29 d^1岁死因顺位为先天性心脏病、肺炎、败血症;~3岁死因顺位为先天性心脏病、意外中毒损伤、传染病;~7岁死因顺位为意外损伤、先天性心脏病、败血症;~15岁死因顺位为恶性肿瘤、意外损伤、先天性心脏病。结论:加强围生期保健,重视家长健康宣教,强化安全意识,防治环境污染,预防感染可以大大减少儿童病死率。

关键词：儿童,住院病死率死亡原因

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19例儿童重症心肌炎诊治体会

中国小儿急救医学 2014年第5期21卷 296-299页

作者：夏天和吴蓉洲张园海陈其项如莲温州医科大学附属第二医院、育英儿童医院儿童心内科 325027

目的探讨儿童重症心肌炎临床特点及治疗方法。方法对我院2005年1月至2012年1月收治的19例儿童重症心肌炎（重症心肌炎组）发病特点、临床表现、诊治经过及预后进行回顾性分析,选择同期在我院体检的正常健康儿童23例为对照组。采用ELIS... 详细信息

目的探讨儿童重症心肌炎临床特点及治疗方法。方法对我院2005年1月至2012年1月收治的19例儿童重症心肌炎（重症心肌炎组）发病特点、临床表现、诊治经过及预后进行回顾性分析,选择同期在我院体检的正常健康儿童23例为对照组。采用ELISA法检测心肌肌钙蛋白（cardiac troponin,CTn）-Ⅰ及血清氨基末端脑利钠肽前体（N-terminal pro-brain natriuretic peptide,NT-proBNP）水平,应用彩色多普勒超声心动图检查了解左室射血分数和左室短轴缩短率变化。结果重症心肌炎组患儿CTn-Ⅰ为（18.67 ±12.31） ng/ml,显著高于正常对照组[（0.02 ±0.01） ng/ml],差异有统计学意义（P＜0.05）。与急性期相比,病程第7天CTn-Ⅰ为（0.55±0.24） ng/ml,呈逐渐下降趋势,第14天基本接近正常[（0.06±0.03） ng/ml],差异有统计学意义（P＜0.05）。重症心肌炎组NT-proBNP较对照组明显增高[（3 067.26 ±902.79） pg/ml vs （80.04±17.79） pg/ml,P＜0.05]。与急性期相比,病程第7天NT-proBNP为（648.63±342.37） pg/ml,病程第14天基本接近正常[（213.58±129.51） pg/ml]（P＜0.05）。重症心肌炎组患儿左室射血分数[（52.63±6.98）％vs（71.39±2.41）％]及左室短轴缩短率[（32.1±2.97）％vs（40.04±2.31）％]明显低于正常对照组,差异均有统计学意义（P均＜0.05）。结论儿童重症心肌炎起病急,病情重,病死率高,在综合治疗基础上早期应用肾上腺素皮质激素和丙种球蛋白,必要时安装临时起搏器,可改善预后。

关键词：重症心肌炎诊断治疗儿童

2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents

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Science Bulletin 2018年第23期63卷 1558-1564页

作者： Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li xiaohui Li xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu tianhe xia Yanyan xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi xia Yu xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu Department of Pediatric Cardiovasoloy Children's Medical CenterThe Second Xiangya HospitalCentral South UniversityChangsha 410071China Department of Pediatrics Peking University First HospitalBeijing 100034China Department of Pediatric Cardiology Children's Hospital of Fudan UniversityShanghai 201102China Department of Pediatric Cardiology Shanghai Children's HospitalShanghai 207102China of Pediatrics the 2nd Hospital of Lanzhou UniversityLanzhou 730020China Department of Pediatric Cardiology Capital Institute of PediatricsBezjing 100020China Department of Pediatrics Jilin University First HospitalChangchun 130021China Chinese Pediatric Cardiology Society Chinese Pediatric Society(CPCS)Chinese Medical Association(CMA)China Committee on Pediatric Syncope Pediatricians BranchChinese Medical Doctor Association(CMDA)China Pediatric Cardiology Society Beijing Pediatric SocietyBeijing Medical Association(BMA)China Committee on Pediatric Cardiology Chinese College of Cardiovascular PhysiciansChinese Medical Doctor Association(CMDA)China

Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department *** syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up.

关键词： CPCS Children Adolescent syncope