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Optimization of Extraction Process and Determination of Antioxidant Activity of Myrica ruba Leaf Pigment by Orthogonal Design

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Medicinal Plant 2017年第5期8卷 45-48页

作者： Gongxiao MO Mengyao LIN Jiaming WANG Yuandan LAN Xunjie LIANG Xiang WANG Qiumeng WEI suoyi HUANG College of Clinical Medicine Youjiang Medical University for Nationalities College of Pharmacy Youjiang Medical University for Nationalities Key Laboratory of Guangxi Universities on National Medicine in Youjiang River Basin

[Objectives] To determine the optimum conditions and antioxidant activity of Myrica ruba leaf pigment,and provide reference for further development and use of Myrica ruba leaves. [Methods]Pigment of Myrica ruba leaves was extracted by ethanol extraction method. Absorbance was measured at wavelength of 300 nm. Effects of ethanol volume fraction,solid-to-liquid ratio,temperature,and extraction time were studied. L_9( 3~3) orthogonal experiment was carried out to optimize the extraction process,and DPPH scavenging ability was measured.[Results]The optimum extraction process of Myrica ruba leaf pigment was as follows: the ethanol volume fraction was 50%; the solid-to-liquid ratio was 1: 60( w/v),extraction temperature 70℃; extraction time was 60 min; after the concentration of purified Myrica ruba leaf pigment reached 0. 4455 mg/mL,the DPPH scavenging ability was basically the same as the same concentration Vc.[Conclusions] The experiment indicates that the optimized extraction process has a high yield rate,and Myrica ruba leaf pigment has high anti-oxidant activity.

关键词： Myrica ruba leaf Pigment Antioxidant activity Orthogonal design

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龙脷叶萃取部位的抗肿瘤活性筛选研究

右江民族医学院学报 2022年第4期44卷 519-522页

作者：李童谢欣雷智冬黄锁义右江民族医学院基础医学院广西百色533000 右江民族医学院药学院广西百色533000 广西中医药大学药学院广西南宁530000 广西高校右江流域特色民族药研究重点实验室广西百色533000

目的研究龙脷叶石油醚、乙酸乙酯、正丁醇和水溶剂萃取部位的抗肿瘤活性。方法龙脷叶的乙醇提取物分别经石油醚、乙酸乙酯、正丁醇和水溶剂萃取、浓缩后得到各部位浸膏,用CCK-8法检测这4个不同极性萃取部位对3种肿瘤细胞株体外生长的抑... 详细信息

目的研究龙脷叶石油醚、乙酸乙酯、正丁醇和水溶剂萃取部位的抗肿瘤活性。方法龙脷叶的乙醇提取物分别经石油醚、乙酸乙酯、正丁醇和水溶剂萃取、浓缩后得到各部位浸膏,用CCK-8法检测这4个不同极性萃取部位对3种肿瘤细胞株体外生长的抑制作用,并计算其半数抑制浓度(IC_(50)值)。结果石油醚萃取部位和乙酸乙酯萃取部位对3种肿瘤细胞株的体外生长均有抑制作用,其中乙酸乙酯萃取部位的抗肿瘤活性最好,乙酸乙酯萃取部位对人胃癌(SGC-7901)、人乳腺癌(MCF-7)、人肝癌(BEL-7404)细胞株的IC_(50)值分别为0.60 mg/mL,0.59 mg/mL,0.49 mg/mL。结论龙脷叶乙醇提取物的石油醚、乙酸乙酯萃取部位均具有一定的抗肿瘤作用,其中乙酸乙酯萃取部位抗肿瘤活性最好。

关键词：龙脷叶肿瘤细胞抗肿瘤活性筛选

Optimization of Ultrasonic Extraction Process of Oenanthe benghalensis Polysaccharide by Orthogonal Test

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Medicinal Plant 2018年第5期9卷 23-26页

作者： Liufu LU Aiyue LUO Bingyang LIN Zhenlian XIE Guangyu GAN Xiuzhen HUANG Zhidong LEI Shimei CHEN suoyi HUANG School of Clinical Medicine Youjiang Medical University for Nationalities School of Pharmacy Youjiang Medical University for Nationalities School of Pharmacy Guangxi University of Chinese Medicine Basic Medical College Youjiang Medical University for Nationalities Key Laboratory of Characteristic Ethnic Medicine Research in Youjiang River Basin of Guangxi Colleges and Universities Youjiang Medical University for Nationalities

[Objectives] The research aimed to explore extraction method of Oenanthe benghalensis polysaccharide,and determine the optimal process of its ultrasonic extraction by orthogonal test. [Methods] Phenol-sulfuric acid method was used to determine polysaccharide's content. Based on single-factor experiment and orthogonal test,the influences of solid-liquid ratio( A),ultrasonic extraction temperature( B),ultrasonic power( C) and ultrasonic time( D) on extraction rate of O. benghalensis polysaccharide were studied,and the optimal process of ultrasonic extraction was selected. [Results] The influence order of solid-liquid ratio( A),ultrasonic extraction temperature( B),ultrasonic power( C) and ultrasonic time( D) on extraction rate of O. benghalensis polysaccharide was A > D > B > C. Via the orthogonal test,it was determined that the optimal extraction condition of O. benghalensis polysaccharide was as below: solid-liquid ratio 1∶ 25( g/mL),extraction temperature 60℃,extraction power 80 W,extraction time 40 min. Under the condition,extraction rate of O. benghalensis polysaccharide was9. 80%. [Conclusions] The process could provide the reference basis for extracting and producing O. benghalensis polysaccharides.

关键词： Orthogonal test Oenanthe benghalensis Polysaccharide Ultrasonic method

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小茴香有效成分提取方法的研究进展

食品工业 2020年第1期41卷 260-263页

作者：陈石梅黄锁义右江民族医学院基础医学院百色533000 右江民族医学院药学院百色533000 右江民族医学院广西高校右江流域特色民族药研究重点实验室百色533000

小茴香作为一种分布广泛的草药,既具有很高的药用价值,也具有一定的食用价值,其主要有效成分为茴香精油、挥发油、黄酮、生物碱等,具有抗炎镇痛、抗菌、抗氧化、增加胃肠蠕动、调节免疫功能、抗肿瘤等作用。因此选择高效合理的提取方法... 详细信息

小茴香作为一种分布广泛的草药,既具有很高的药用价值,也具有一定的食用价值,其主要有效成分为茴香精油、挥发油、黄酮、生物碱等,具有抗炎镇痛、抗菌、抗氧化、增加胃肠蠕动、调节免疫功能、抗肿瘤等作用。因此选择高效合理的提取方法具有重要意义。综述近10年有关小茴香有效成分的提取方法,并展望小茴香的发展前景,为进一步开发和利用小茴香提供一定理论依据。

关键词：小茴香有效成分提取方法

A Preliminary Study on Anti-inflammatory and Analgesic Effects of Millettia speciosa and Tinpspora sinensis and Their Compatibility

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Medicinal Plant 2020年第1期11卷 32-36页

作者： Shibo ZHAO suoyi HUANG Xinpeng CHEN Huanhui DENG Zhi PAN Kairen XIE Haoming YU Fen MO College of Clinical Medicine Youjiang Medical University for NationalitiesBaise 533000China College of Pharmacy Youjiang Medical University for NationalitiesBaise 533000China Youjiang Medical University for Nationalities Key Laboratory of Guangxi Universities on National Medicine in Youjiang River BasinBaise 533000China

[Objectives]To study the anti-inflammatory and analgesic effects of Millettia speciosa and Tinpspora sinensis and their compatibility.[Methods](i)In the glacial acetic acid writhing experiment,60 SPF-grade Kunming mice were adopted,and the mice were randomly divided into 6 groups at male-female ratio of 1∶1,namely,the blank control group,M.speciosa group,T.sinensis group,M.speciosa compatible with T.sinensis group at the ratio of 1∶2(expressed as 1∶2 compatibility group),M.speciosa compatible with T.sinensis group at the ratio of 1∶1(expressed as 1∶1 compatibility group),and M.speciosa compatible with T.sinensis group at the ratio of 2∶1(expressed as 2∶1 compatibility group),12 mice for each group.Mice of the experimental groups were administered at a dose of 20 mL/kg,and the corresponding concentration of the Chinese medicine extract was given at 1 g/mL.The control group was administered with an equal volume of 0.9%physiological saline,and was intragastrically administered once every 24 h for 14 d.After intragastric administration for one hour on day 14,intraperitoneal injection of 0.5%glacial acetic acid solution was performed to induce pain.(ii)In the hot plate experiment,60 Kunming female SPF mice were adopted,grouped,intragastrically administered with the same glacial acetic acid writhing experiment for 14 d.After intragastric administration for one hour on day 14,the mice were placed on a hot plate apparatus at(55±0.5)℃.to measure the time of licking their hind feet.(iii)In the anti-inflammatory experiment,60 Kunming SPF mice were adopted,grouped,intragastrically administered with the same glacial acetic acid writhing experiment for 14 d.After intragastric administration for one hour on day 14,xylylene was administered to the left ears of mice at a dose of 50μL/piece to induce inflammation.[Results](i)In the glacial acetic acid writhing experiment,compared with the blank control group,the experimental group showed analgesic effects.Specifically,M.speciosa group,T.sinensis group,1∶2 compatibility group,1∶1 c

关键词： Millettia speciosa Tinpspora sinensis Compatibility Anti-inflammatory effects Analgesic effects

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民族医学院校大学生思想状况特点及对策思考

广西高教研究 2001年第2期 7-9页

作者：黄锁义广西右江民族医学院化学教研室广西百色533000

对右江民族医学院大学生的思想状况普遍存在的问题及特点进行了分析和探讨。

关键词：少数民族大学生思想状况广西右江民族医学院思想工作特点勤工助学活动

Experimental Study on Analgesia and Hemostasis of Paris polyphylla,Scutellaria baicalensis and Their Compatibility

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Medicinal Plant 2020年第2期11卷 33-37页

作者： Shibo ZHAO suoyi HUANG Zhenjing SI Lanlan GAN Shusheng HUANG Ye FU Jianjie LAN Xueying YU Yuekong CEN College of Clinical Medicine Youjiang Medical University for Nationalities Baise 533000 China College of Pharmacy Youjiang Medical University for Nationalities Baise 533000 China Key Laboratory of Guangxi Universities on National Medicine in Youjiang River Youjiang Medical University for Nationalities Baise 533000 China

[Objectives]The analgesic and hemostatic effects of Paris polyphylla,Scutellaria baicalensis,and their compatibility were studied.[Methods](i)In the experiment on the writhing of mice induced by acetic acid,60 Kunming SPF mice were randomly selected,and were randomly divided into 6 groups according to the male-to-female ratio of 1∶1,including blank control group,P.polyphylla group,S.baicalensis group,and three P.polyphylla and S.baicalensis compatibility groups(with the ratio of 1∶2,1∶1 and 1∶2).There were 10 mice in each group.In the experimental groups,the mice were given 1 g/mL Chinese medicine extract according to a dose of 20 mL/kg.In the control group,the mice were given 0.9%normal saline in equal volumes.Gavage was performed one time every 24 h and lasted for 14 d.1 h after the gavage on the 14th day,they were given 0.5%glacial acetic acid solution via intraperitoneal injection.(ii)In the hot plate experiment,60 Kunming SPF mice were selected,and the ratio of male to female,grouping,administration and gavage were the same as those of the glacial acetic acid-induced writhing experiment.Gavage was lasted for 14 d.1 h after the gavage on the 14th day,the mice were placed on a hot plate apparatus at(55±0.5)℃,and the time of licking hindfoot was measured.(iii)The hemostatic effect was explored through three experimental methods of tail hemostasis,femoral artery hemostasis and liver hemostasis.60 Kunming SPF mice were taken in each of the three experiments,and the male-female ratio and grouping were the same as above.The powder was covered on the surface of the wound sites,and the bleeding was observed.The hemostatic time was recorded,and the hemostatic time was recorded as 3 min if it exceeded 3 min.[Results](i)In the acetic acid-induced writhing experiment,compared with the blank control group,the pain of mice in the experimental groups was inhibited.Among them,P.polyphylla group,S.baicalensis group,compatibility 1∶2 group,compatibility 1∶1 group,and compatibility 2∶1 group had significant effects(P<0.05),and

关键词： Paris polyphylla Scutellaria baicalensis Compatibility Analgesic effect Hemostatic effect

Comparison of Extraction Methods for Total Alkaloids from Abrus Cantoniensis Hance

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Medicinal Plant 2017年第2期8卷 19-21页

作者： Mengyao LIN Qiumeng WEI Xiaoxia HUANG Jiaming WANG suoyi HUANG Luyao WANG Yan LIU Gongxiao MO Xunjie LIANG Xiaoting MA College of Clinical Medicine Youjiang Medical University for Nationalities College of Pharmacy Youjiang Medical University for Nationalities Key Laboratory of Guangxi Universities on National Medicine in Youjiang River Basin

[Objectives] To compare different extraction methods for total alkaloids from Abrus Cantoniensis Hance taking chloroform as solvent. [Methods] The ethanol extraction method,ultrasonic extraction method,acid water extraction method,and Soxhlet extraction method were used to extract total alkaloids,and the total alkaloids of Abrus Cantoniensis Hance were measured at 238 nm wavelength. [Results] The extraction rate of ethanol extraction method was 14. 76 mg/100 g; the extraction rate of ultrasonic extraction method was 56. 85 mg/100 g; the extraction rate of acid water extraction method was 56. 63 mg/100 g; the extraction rate of Soxhlet extraction method was 58. 19 mg/100 g.[Conclusions]Results indicate that Soxhlet extraction method can obtain the highest extraction rate.

关键词： Total alkaloids Abrus Cantoniensis Hance Extraction methods Comparison

Extraction of Herba Lycopi Polysaccharide and Study of Its Antioxidant Activity by Cellulase Method

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Medicinal Plant 2017年第3期8卷 28-30,34页

作者： Tao HUANG Zhaoping ZHANG Zudi YAN Yunda LI Yuanhui LI Ying HUANG Tingzhang HUANG Qiaorong YU Mengyao LIN Wende LU suoyi HUANG Clinical College Youjiang Medical University for Nationalities College of Pharmacy Youjiang Medical University for Nationalities College of Pharmacy Guangxi University of Chinese Medicine Key Laboratory of Guangxi's Colleges for the Study of Characteristic Ethnic Medicine in Youjiang River Basin

[Objectives] To optimize the extraction process of Herba Lycopi polysaccharide by cellulase,and study its antioxidant activity.[Methods]The single factor test and orthogonal test were conducted to study the effect of p H,enzyme dosage,enzymolysis temperature and extraction time on the extraction of Herba Lycopi polysaccharide. And the test was done about its scavenging effect on DPPH radicals and superoxide anions,and the antioxidant activity was evaluated. [Results] The optimum extraction process by cellulase method was as follows: p H value of 7; enzyme dosage of 4 ml; enzymolysis temperature of 40 ℃; extraction time of 2. 5 h. The average extraction rate of Herba Lycopi polysaccharide was 10. 70%,and RSD was 0. 17%. The antioxidant activity test showed that Herba Lycopi polysaccharide had a significant scavenging effect on radicals. [Conclusions] Herba Lycopi polysaccharides had strong antioxidant activity,and could provide a scientific basis for the development and utilization of Herba Lycopi medicinal resources.

关键词： Herba Lycopi Polysaccharides Cellulase Orthogonal design Antioxidant activity