Optimization of Extraction Process and Determination of Antioxidant Activity of Myrica ruba Leaf Pigment by Orthogonal Design

作     者：Gongxiao MO Mengyao LIN Jiaming WANG Yuandan LAN Xunjie LIANG Xiang WANG Qiumeng WEI Suoyi HUANG 

作者机构：College of Clinical MedicineYoujiang Medical University for Nationalities College of PharmacyYoujiang Medical University for Nationalities Key Laboratory of Guangxi Universities on National Medicine in Youjiang River Basin 

出 版 物：《Medicinal Plant》 (药用植物：英文版)

年 卷 期：2017年第8卷第5期

页      面：45-48页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by Student’s Platform for Innovation and Entrepreneurship Training Program of Guangxi Zhuang Autonomous Region,China in 2016(201610599033) Innovation and Entrepreneurship Ethnical Medicine Teaching Team Program of Guangxi Zhuang Autonomous Region,China(Gui Jiao Gao Jiao93&Gui Jiao Gao Jiao6) 

主　　题：Myrica ruba leaf Pigment Antioxidant activity Orthogonal design 

摘      要：[Objectives] To determine the optimum conditions and antioxidant activity of Myrica ruba leaf pigment,and provide reference for further development and use of Myrica ruba leaves. [Methods]Pigment of Myrica ruba leaves was extracted by ethanol extraction method. Absorbance was measured at wavelength of 300 nm. Effects of ethanol volume fraction,solid-to-liquid ratio,temperature,and extraction time were studied. L_9( 3~3) orthogonal experiment was carried out to optimize the extraction process,and DPPH scavenging ability was measured.[Results]The optimum extraction process of Myrica ruba leaf pigment was as follows: the ethanol volume fraction was 50%; the solid-to-liquid ratio was 1: 60( w/v),extraction temperature 70℃; extraction time was 60 min; after the concentration of purified Myrica ruba leaf pigment reached 0. 4455 mg/mL,the DPPH scavenging ability was basically the same as the same concentration Vc.[Conclusions] The experiment indicates that the optimized extraction process has a high yield rate,and Myrica ruba leaf pigment has high anti-oxidant activity.