Optimization of Ultrasonic Extraction Process of Oenanthe benghalensis Polysaccharide by Orthogonal Test

作     者：Liufu LU Aiyue LUO Bingyang LIN Zhenlian XIE Guangyu GAN Xiuzhen HUANG Zhidong LEI Shimei CHEN Suoyi HUANG 

作者机构：School of Clinical MedicineYoujiang Medical University for Nationalities School of PharmacyYoujiang Medical University for Nationalities School of PharmacyGuangxi University of Chinese Medicine Basic Medical CollegeYoujiang Medical University for Nationalities Key Laboratory of Characteristic Ethnic Medicine Research in Youjiang River Basin of Guangxi Colleges and UniversitiesYoujiang Medical University for Nationalities 

出 版 物：《Medicinal Plant》 (药用植物：英文版)

年 卷 期：2018年第9卷第5期

页      面：23-26页

学科分类：10[医学] 

基　　金：Supported by Chinese Medicine Chemistry Construction Project in"12th Fiveyear" Key Disciplines of Chinese Medicine,State Administration of Traditional Chinese Medicine(Guozhongyiyaorenjiaofa32) Pharmaceutical Chemistry Construction Project of Guangxi Key Discipline(Guijiaokeyan16) Construction Program of Technology Innovation Capability Upgrading Project in Guangxi Colleges and Universities(Guijiaokeyan5) Student's Platform for Innovation and Entrepreneurship Training Program in Youjiang Medical University for Nationalities in 2017(201710599110) 

主　　题：Orthogonal test Oenanthe benghalensis Polysaccharide Ultrasonic method 

摘      要：[Objectives] The research aimed to explore extraction method of Oenanthe benghalensis polysaccharide,and determine the optimal process of its ultrasonic extraction by orthogonal test. [Methods] Phenol-sulfuric acid method was used to determine polysaccharide s content. Based on single-factor experiment and orthogonal test,the influences of solid-liquid ratio( A),ultrasonic extraction temperature( B),ultrasonic power( C) and ultrasonic time( D) on extraction rate of O. benghalensis polysaccharide were studied,and the optimal process of ultrasonic extraction was selected. [Results] The influence order of solid-liquid ratio( A),ultrasonic extraction temperature( B),ultrasonic power( C) and ultrasonic time( D) on extraction rate of O. benghalensis polysaccharide was A D B C. Via the orthogonal test,it was determined that the optimal extraction condition of O. benghalensis polysaccharide was as below: solid-liquid ratio 1∶ 25( g/mL),extraction temperature 60℃,extraction power 80 W,extraction time 40 min. Under the condition,extraction rate of O. benghalensis polysaccharide was9. 80%. [Conclusions] The process could provide the reference basis for extracting and producing O. benghalensis polysaccharides.