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Optimization of Ultrasonic Extraction Process of oenanthe benghalensis Polysaccharide by Orthogonal Test

Medicinal Plant 2018年第5期9卷 23-26页

作者： Liufu LU Aiyue LUO Bingyang LIN Zhenlian XIE Guangyu GAN Xiuzhen HUANG Zhidong LEI Shimei CHEN Suoyi HUANG School of Clinical Medicine Youjiang Medical University for Nationalities School of Pharmacy Youjiang Medical University for Nationalities School of Pharmacy Guangxi University of Chinese Medicine Basic Medical College Youjiang Medical University for Nationalities Key Laboratory of Characteristic Ethnic Medicine Research in Youjiang River Basin of Guangxi Colleges and Universities Youjiang Medical University for Nationalities

[Objectives] The research aimed to explore extraction method of oenanthe benghalensis polysaccharide,and determine the optimal process of its ultrasonic extraction by orthogonal test. [Methods] Phenol-sulfuric acid method was used to determine polysaccharide's content. Based on single-factor experiment and orthogonal test,the influences of solid-liquid ratio( A),ultrasonic extraction temperature( B),ultrasonic power( C) and ultrasonic time( D) on extraction rate of O. benghalensis polysaccharide were studied,and the optimal process of ultrasonic extraction was selected. [Results] The influence order of solid-liquid ratio( A),ultrasonic extraction temperature( B),ultrasonic power( C) and ultrasonic time( D) on extraction rate of O. benghalensis polysaccharide was A > D > B > C. Via the orthogonal test,it was determined that the optimal extraction condition of O. benghalensis polysaccharide was as below: solid-liquid ratio 1∶ 25( g/mL),extraction temperature 60℃,extraction power 80 W,extraction time 40 min. Under the condition,extraction rate of O. benghalensis polysaccharide was9. 80%. [Conclusions] The process could provide the reference basis for extracting and producing O. benghalensis polysaccharides.

关键词： Orthogonal test oenanthe benghalensis Polysaccharide Ultrasonic method

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Pretreated Oenan the Javanica extract increases anti-inflammatory cytokines, attenuates gliosis, and protects hippocampal neurons following transient global cerebral ischemia in gerbils

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Neural Regeneration Research 2019年第9期14卷 1536-1543页

作者： Joon Ha Park In Hye Kim Ji Hyeon Ahn YooHun Noh Sung-Su Kim Tae-Kyeong Lee Jae-Chul Lee Bich-Na Shin Tae Heung Sim Hyun Sam Lee Jeong Hwi Cho In Koo Hwang Il Jun Kang Jong Dai Kim Moo-Ho Won Department of Biomedical Science and Research Institute for Bioscience and Biotechnology Hallym UniversityChuncheonGangwonRepublic of Korea Famenity Company GwacheonGeyonggiRepublic of Korea Department of Neurobiology School of MedicineKangwon National UniversityChuncheonGangwonRepublic of Korea Danchunok Company ChuncheonGangwonRepublic of Korea Department of Histology College of Veterinary MedicineChonbuk National UniversityIksanJeollabuk.doRepublic of Korea Department of Anatomy and Cell Biology College of Veterinary Medicineand Research Institute for Veterinary ScienceSeoul National UniversitySeoulRepublic of Korea Department of Food Science and Nutrition Hallym UniversityChuncheonGangwonRepublic of Korea Division of Food Biotechnology School of BiotechnologyKangwon National UniversityChuncheonGangwonRepublic of Korea

Recently,we have reported that oenanthe javanica extract(OJE)displays strong neuroprotective effect against ischemic damage after transient global cerebral ***,neuroprotective mechanisms of OJE have not been fully ***,this study investigated the neuroprotection of OJE in the hippocampal CA1 area and its anti-inflammatory activity in gerbils subjected to 5 minutes of transient global cerebral *** treated the animals by intragastrical injection of OJE(100 and 200 mg/kg)once daily for 1 week prior to transient global cerebral *** of OJE was observed by immunohistochemistry for neuronal nuclear antigen and histofluorescence staining for Fluoro-Jade *** of glial fibrillary acidic protein and ionized calcium-binding adapter molecule 1 was done for astrocytosis and microgliosis,*** investigate the neuroprotective mechanisms of OJE,we performed immunohistochemistry of tumor necrosis factor-alpha and interleukin-2 for pro-inflammatory function and interleukin-4 and interleukin-13 for anti-inflammatory *** we treated the animals by intragastrical administration of 200 mg/kg of OJE,hippocampal CA1 pyramidal neurons were protected from transient global cerebral ischemia and cerebral ischemia-induced gliosis was inhibited in the ischemic hippocampal CA1 *** also found that interleukin-4 and-13 immunoreactivities were significantly increased in pyramidal neurons of the ischemic CA1 area after OJE pretreatment,and the increased immunoreactivities were sustained in the CA1 pyramidal neurons after transient global cerebral ***,OJE pretreatment did not increase interleukin-2 and tumor necrosis factor-alpha immunoreactivities in the CA1 pyramidal *** findings suggest that pretreatment with OJE can protect neurons and attenuate gliosis from transient global cerebral ischemia via increasing expressions of interleukin-4 *** experimental plan of this study was rev

关键词： oenanthe javanica extract transient global cerebral ischemia hippocampus ischemic damage cerebral ischemia neuroprotection glial activation pro-inflammatory cytokines anti-inflammatory cytokines inflammation neural regeneration

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