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Identification of Resistance to Pathogenesis Related Protein GmPR1L in Tobacco Botrytis cinerea Infection

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Phyton-International Journal of Experimental Botany 2023年第6期92卷 1907-1920页

作者： Yeyao Du Ye Zhang Yang Song Zhuo Zhang sujie fan Hanzhu Zhang Piwu Wang College of Agronomy Jilin Agricultural UniversityChangchunChina Northeast Institute of Geography and Agroecology Chinese Academy of SciencesChangchunChina

Soybean(Glycine max(Linn.)Merr.)annual leguminous crop is cultivated all over the *** occurrence of diseases has a great impact on the yield and quality of *** this study,based on the RNA-seq of soybean variety M18,a complete CDS(Coding sequence)GmPR1L of the pathogenesis-related protein 1 family was obtained,which has the ability to resist fungal *** overexpression vector and interference expression vector were transferred into tobacco NC89,and the resistance of transgenic tobacco(Nicotiana tabacum L.)to Botrytis cinerea infection was *** results show that:Compared with the control,the activities of related defense enzymes SOD(Superoxide dismutase),POD(Peroxidase),PAL(L-phenylalanine ammonia-lyase)and PPO(Polyphenol oxidase)in the over-expressed transgenic tobacco OEA1 and OEA2 increased to different degrees,and increased significantly at different infection time *** activities of defense enzymes in the interfering strains IEA1 and IEA2 were significantly lower than those in the control *** results of resistance level identification showed that the disease spot rate of OEA1 was significantly lower than that of the control line,and the disease spot rate of OEA2 was significantly lower than that of the control *** plaque rate of the interfering expression line IEA1-IEA2 was significantly higher than that of the control *** is preliminarily believed that the process related protein GmPR1L can improve the resistance of tobacco to ***.

关键词： Soybean pathogenesis-related protein defense enzymes Botrytis cinerea

A Novel Pathogenesis-Related Class 10 Protein Glym41,Increases Resistance upon Phytophthora sojae Infection in Soybean(Glycine max[L.]Merr.)

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A Novel Pathogenesis-Related Class 10 Protein Glym41,Increas...

中国作物学会——2015年学术年会

作者： sujie fan Liangyu Jiang Lidong Dong Pengfei Xu Shuzhen Zhang Soybean Research Institute Northeast Agricultural University

Phytophthora root and stem rot of soybean,caused by Phytophthora sojae(***),is a destructive disease in many soybean planting regions *** a previous study,an expressed sequence tag(EST)homolog of the major allergen Pru ar 1 in apricot(Prunus armeniaca)was identified up-regulated in the highly resistant soybean’Suinong 10’infected with ***,the full length of the EST was isolated using rapid amplification of cDNA ends(RACE).It showed the highest homolgy of 53.46%with Gly m 4after comparison with the eight soybean allergen families reported and was named Gly m 4-like(Gly m 4l,GenBank accession ***913577.1).The cDNA full length of Gly m 4l was 707 bp containing a 474 bp open reading frame encoding a polypeptide of 157 amino *** analysis suggests that Gly m 41contains a conserved’P-loop’(phosphate-binding loop)motif at residues 47-55 aa and a Bet v 1 domain at residues 87-120 *** transcript abundance of Gly m 4l was significantly induced by ***,salicylic acid(SA),NaCl,and also responded to methyl jasmonic acid(MeJA)and ethylene(ET).The recombinant Gly m 41 protein showed RNase activity and displayed directly antimicrobial activity that inhibited hyphal growth and reduced zoospore release in *** analyses showed that the RNase activity of the recombinant protein to degrading tRNA was significantly affected in the presence of ***-expression of Gly m 4l in susceptible’Dongnong 50’soybean showed enhanced tolerance to *** results indicated that Gly m 41 protein played an important role in the defense of soybean against *** infection.

关键词： Glycine max Phytophthora sojae Pathogenesis-Related Class 10 Protein

Overexpression of GmERF5,a new member of the soybean EAR motif-containing ERF transcription factor,enhances resistance to Phytophthora sojae in soybean

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Overexpression of GmERF5,a new member of the soybean EAR mot...

中国作物学会——2015年学术年会

作者： Lidong Dong sujie fan Liangyu Jiang Qun Cheng Pengfei Xu Shuzhen Zhang Soybean Research Institute Key Laboratory of Soybean Biology of Chinese Education MinistryNortheast Agricultural University

Phytophthora root and stem rot of soybean[Glycine max(L.)Merr.],caused by Phytophthora sojae Kaufmann and Gerdemann,is a destructive disease throughout the soybean planting regions in the ***,we report insights into the function and underlying mechanisms of a novel ethylene response factor(ERF)in soybean,namely GmERF5,in host responses to ***5-overexpressmg transgenic soybean exhibited significantly enhanced resistance to *** and positively regulated the expression of the PR10,PR1-1,and PR10-1 *** analysis suggested that GmERF5 contains an AP2/ERF domain of 58 aa and a conserved ERF-associated amphiphilic repression(EAR)motif in its C-terminal *** stress treatments,GmERF5 was significantly induced by ***,ethylene(ET),abscisic acid(ABA),and salicylic acid(SA).The activity of the GmERF5 promoter(GmERF5P)was upregulated in tobacco leaves with ET,ABA,Phytophthora nicotianae,salt,and drought treatments,suggesting that GmERF5 could be involved not only in the induced defence response but also in the ABA-mediated pathway of salt and drought ***5 could bind to the GCC-box element and act as a repressor of gene *** was targeted to the nucleus when transiently expressed in Arabidopsis ***5 interacted with a basic helix-loophelix transcription factor(GmbHLH)and eukaryotic translation initiation factor(GmEIF)both in yeast cells and in *** the best of our knowledge,GmERF5 is the first soybean EAR motif-containing ERF transcription factor demonstrated to be involved in the response to pathogen infection.

关键词： EAR Glycine max GmERF5 Phytophthora sojae AP2/ERF

Laser Iridoplasty Using in Deferent Phases of Acute Angle-closure Glaucoma:a Non-randomized Clinical Trial

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Laser Iridoplasty Using in Deferent Phases of Acute Angle-cl...

中华医学会第十二届全国眼科学术大会

作者： Liang Yuanbo fan sujie Sun Xia Liu Wenru Li Sizhen Sun Lanping Wang Ningli Beijing Tongren Hospital 100730

Purpose:to investigate the efficacy of laser iridoplasty per- formed in different phase of acute primary angle closure glau- ***:non—randomized,controlled clinical trial. Participants and Methods:138 patients with 16... 详细信息

关键词： :acute angle一closure glaucoma Iaser iridotomy laser iridoPlasty clinical trial

Two-staged Combination of Laser Iridotomy and Iridoplasty Versus Laser lridotomy in Chronic Angle-closure Glaucoma

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Two-staged Combination of Laser Iridotomy and Iridoplasty Ve...

中华医学会第十二届全国眼科学术大会

作者： liang Yuanbo Sun Xia fan sujie Sun Lanping Li Sizhen Liu Wenru Wang Ningli Beijing Tongren Hospital 100073

Objectives: to compare the Results: of two-staged combina- tion of laser iridotomy and iridoplasty with laser iridotomy in chronic angle closureglaucoma. Design: parallel randomized, controlled, clinical trialParticip...

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The Management for Primary Angle Closure Glaucoma in Mainland of China-a National Questionnaire of Survey

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The Management for Primary Angle Closure Glaucoma in Mainlan...

中华医学会第十二届全国眼科学术大会

作者： Liang Yuanbo Li Sizhen fan sujie Lei Kun Liu Yuhen Sun Xia Sun Lanping Wang Ningli Beijing Tongren Hospital 100730

Objectives:to provide a profile of the actual status of the clin- ical practice of primary angle closure glaucoma(PACG)in Mainland of ***:non—randomized sample,ques- *** and Methods:In glaucoma section of 10th Congre... 详细信息

关键词： :primary angle一closure glaucoma ques- tionnaire ocular surgery.

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碱激发矿渣-玻璃粉基泡沫混凝土性能研究

硅酸盐通报 2022年第1期41卷 226-234页

作者：龚建清杨倩郭丽范苏杰蔡光伟湖南大学土木工程学院长沙410082 湖南大学绿色先进土木工程材料及应用技术湖南省重点实验室长沙410082 长沙市轨道交通集团有限公司长沙410082

采用玻璃粉部分替代矿渣制备碱激发胶凝材料,研究了玻璃粉含量(10%、20%、30%、40%,质量分数)对碱激发矿渣-玻璃粉基(AASG)泡沫混凝土性能的影响。对AASG泡沫混凝土流动性、抗压强度、干燥收缩、吸水率、软化系数和抗冻性进行了研究,并... 详细信息

采用玻璃粉部分替代矿渣制备碱激发胶凝材料,研究了玻璃粉含量(10%、20%、30%、40%,质量分数)对碱激发矿渣-玻璃粉基(AASG)泡沫混凝土性能的影响。对AASG泡沫混凝土流动性、抗压强度、干燥收缩、吸水率、软化系数和抗冻性进行了研究,并通过扫描电子显微镜和X射线衍射仪对机理进行了分析。结果表明:10%~40%掺量的玻璃粉使AASG泡沫混凝土的流动性提高了5.0%~25.6%;抗压强度随玻璃粉掺量的增加先增大再减小,玻璃粉掺量为20%时,7 d和28 d抗压强度最高,与对照组相比分别提高15.0%和23.8%;玻璃粉掺量为20%时,AASG泡沫混凝土的干燥收缩、吸水率、软化系数和抗冻性最佳;SEM分析发现,玻璃粉有助于孔结构的优化和提高微观结构的致密性;XRD分析表明,AASG泡沫混凝土的主要反应产物为C-(N-)A-S-H和水滑石。将玻璃粉作为矿渣的替代品来制备AASG泡沫混凝土是可行的,为其在回填工程和固废利用提供理论支撑。

关键词：碱激发泡沫混凝土玻璃粉抗压强度干燥收缩软化系数抗冻性

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玉米直链淀粉近红外模型的建立

分子植物育种 2017年第6期15卷 2286-2290页

作者：吴晗张力韩笑范素杰丁宝成王铎关淑艳张君吉林农业大学农学院长春130118 双辽市农业局双辽136400

为提高玉米直链淀粉含量育种效率,加速育种进程,本研究使用210份玉米材料,利用近红外谷物分析仪,建立玉米直链淀粉含量快速检测模型。结果表明:采用改进偏最小二乘法回归分析技术(MPLS),同时采用"SNV+Dtrend"的散射处理,加"1.***.4.1"的... 详细信息

为提高玉米直链淀粉含量育种效率,加速育种进程,本研究使用210份玉米材料,利用近红外谷物分析仪,建立玉米直链淀粉含量快速检测模型。结果表明:采用改进偏最小二乘法回归分析技术(MPLS),同时采用"SNV+Dtrend"的散射处理,加"1.***.4.1"的参数处理所得的结果最好,其定标标准偏差(SEC)是0.388,交叉检验误差是(SECV)是1.465,定标决定系数(RSQ)是0.963。验证结果表明:利用该模型的近红外检验结果和化学测定结果绝对误差均在1%以内,表明所建立的定标模型预测能力良好,可用于玉米材料直链淀粉含量的快速测定。

关键词：玉米直链淀粉近红外模型

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不同自然老化时间的大豆种子活力及生理差异

吉林农业大学学报 2021年第3期43卷 283-288页

作者：赵欣欣吴依榕毛瑞琳汪元芬范素杰吉林农业大学农学院长春130118

为研究自然老化过程中大豆种子的活力状况,提高种用价值,以不同自然老化时间(16个月,28个月,40个月)的大豆品种"吉育47"和"吉农29"为试验材料,测定发芽、活力、生长和生理指标。结果表明:随自然老化时间延长,大豆种子的发芽及活力指标... 详细信息

为研究自然老化过程中大豆种子的活力状况,提高种用价值,以不同自然老化时间(16个月,28个月,40个月)的大豆品种"吉育47"和"吉农29"为试验材料,测定发芽、活力、生长和生理指标。结果表明:随自然老化时间延长,大豆种子的发芽及活力指标均呈下降趋势,其中发芽势下降最快。在种子老化至40个月时,"吉育47"和"吉农29"的发芽率已降至79%和82%,苗高和苗重表现为显著低于对照,均不可作为种用。2个大豆品种的过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶(SOD)活性均表现为随老化时间的延长而降低。老化40个月时,"吉育47"和"吉农29"的CAT活性分别为353.3 U/g和320.0 U/g,均降到最低水平,丙二醛(MDA)含量和脯氨酸含量积累至最高值,其中"吉育47"的MDA含量为2.64μmol/g,"吉农29"的MDA含量是2.73μmol/g。因此,自然老化40个月是大豆种子种用价值的临界时间点。

关键词：大豆自然老化种子活力生理指标