A Novel Pathogenesis-Related Class 10 Protein Glym41,Increases Resistance upon Phytophthora sojae Infection in Soybean(Glycine max[L.]Merr.)
作者单位：Soybean Research InstituteNortheast Agricultural University
会议名称：《中国作物学会——2015年学术年会》
会议日期：2015年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学]
关 键 词：Glycine max Phytophthora sojae Pathogenesis-Related Class 10 Protein
摘 要：Phytophthora root and stem rot of soybean,caused by Phytophthora sojae(P.sojae),is a destructive disease in many soybean planting regions worldwide.In a previous study,an expressed sequence tag(EST)homolog of the major allergen Pru ar 1 in apricot(Prunus armeniaca)was identified up-regulated in the highly resistant soybean’Suinong 10’infected with P.sojae.Here,the full length of the EST was isolated using rapid amplification of cDNA ends(RACE).It showed the highest homolgy of 53.46%with Gly m 4after comparison with the eight soybean allergen families reported and was named Gly m 4-like(Gly m 4l,GenBank accession no.HQ913577.1).The cDNA full length of Gly m 4l was 707 bp containing a 474 bp open reading frame encoding a polypeptide of 157 amino acids.Sequence analysis suggests that Gly m 41contains a conserved’P-loop’(phosphate-binding loop)motif at residues 47-55 aa and a Bet v 1 domain at residues 87-120 aa.The transcript abundance of Gly m 4l was significantly induced by P.sojae,salicylic acid(SA),NaCl,and also responded to methyl jasmonic acid(MeJA)and ethylene(ET).The recombinant Gly m 41 protein showed RNase activity and displayed directly antimicrobial activity that inhibited hyphal growth and reduced zoospore release in P.sojae.Further analyses showed that the RNase activity of the recombinant protein to degrading tRNA was significantly affected in the presence of zeatin.Over-expression of Gly m 4l in susceptible’Dongnong 50’soybean showed enhanced tolerance to P.sojae.These results indicated that Gly m 41 protein played an important role in the defense of soybean against P.sojae infection.