The Improvement of Soybean Salt Tolerance by Overexpressed GmPAO1
作者机构:College of AgronomyJilin Agricultural UniversityChangchun130118China
出 版 物:《Phyton-International Journal of Experimental Botany》 (国际实验植物学杂志(英文))
年 卷 期:2023年第92卷第4期
页 面:1109-1124页
核心收录:
学科分类:0710[理学-生物学] 09[农学] 0901[农学-作物学]
基 金:supported by Jilin Province Science and Technology Development Plan Project,Grant No.20190103120JH Jilin Province Science and Technology Development Plan-Outstanding Young Talents Fund Project,Grant No.20190103120J The fourth batch of Jilin Province Youth Science and Technology Talent Support Project,Grant No.QT202020 National Natural Science Foundation of China Projects,Grant No.31801381
主 题:Soybean polyamine oxidase salt tolerance overexpressed
摘 要:Polyamines play an important regulatory role during plant growth and development and adversity stress,and polyamine oxidase(PAO)is involved in polyamine *** this study,an up-regulated polyamine oxidase gene GmPAO1 was obtained by transcriptome sequencing analysis and screening at soybean seedling ***,its expression pattern and function were *** identification results of transgenic GmPAO1 soybean positive lines showed that the relative expression level of GmPAO1 in the overexpressed lines was increased under salt *** increasing stress concentration,the seed germination rate ***,the seed germination rate of the overexpressed lines was significantly higher than that of the control lines,and the phenotypic character of the root systems was also better than that of the control *** measurement of superoxide dismutase(SOD)and peroxidase(POD)activities and malondialdehyde and hydrogen peroxide contents revealed that the overexpressed soybean lines significantly increased the SOD and POD activities,significantly reducing the malondialdehyde *** the hydrogen peroxide content in the transformed plants gradually increased,the hydrogen peroxide content in the overexpression lines was still lower than that in the gene editing *** on this,it was preliminarily judged that GmPAO1 can improve soybean salt tolerance.