Overexpression of GmERF5,a new member of the soybean EAR motif-containing ERF transcription factor,enhances resistance to Phytophthora sojae in soybean
作者单位：Soybean Research InstituteKey Laboratory of Soybean Biology of Chinese Education MinistryNortheast Agricultural University
会议名称：《中国作物学会——2015年学术年会》
会议日期：2015年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治]
关 键 词：EAR Glycine max GmERF5 Phytophthora sojae AP2/ERF
摘 要：Phytophthora root and stem rot of soybean[Glycine max(L.)Merr.],caused by Phytophthora sojae Kaufmann and Gerdemann,is a destructive disease throughout the soybean planting regions in the ***,we report insights into the function and underlying mechanisms of a novel ethylene response factor(ERF)in soybean,namely GmERF5,in host responses to ***5-overexpressmg transgenic soybean exhibited significantly enhanced resistance to *** and positively regulated the expression of the PR10,PR1-1,and PR10-1 *** analysis suggested that GmERF5 contains an AP2/ERF domain of 58 aa and a conserved ERF-associated amphiphilic repression(EAR)motif in its C-terminal *** stress treatments,GmERF5 was significantly induced by ***,ethylene(ET),abscisic acid(ABA),and salicylic acid(SA).The activity of the GmERF5 promoter(GmERF5P)was upregulated in tobacco leaves with ET,ABA,Phytophthora nicotianae,salt,and drought treatments,suggesting that GmERF5 could be involved not only in the induced defence response but also in the ABA-mediated pathway of salt and drought ***5 could bind to the GCC-box element and act as a repressor of gene *** was targeted to the nucleus when transiently expressed in Arabidopsis ***5 interacted with a basic helix-loophelix transcription factor(GmbHLH)and eukaryotic translation initiation factor(GmEIF)both in yeast cells and in *** the best of our knowledge,GmERF5 is the first soybean EAR motif-containing ERF transcription factor demonstrated to be involved in the response to pathogen infection.