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Screening and identification of interacting proteins with hepatitis B virus core protein in leukocytes and cloning of new gene C1

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World Journal of Gastroenterology 2006年第7期12卷 1043-1048页

作者： shu-mei lin Jun Cheng Yin-Ying Lu shu-lin Zhang Qian Yang Tian-Yan Chen Min Liu lin Wang Department of Infectious Diseases The First Affiliated Hospital of Medical College of Xi'an Jiaotong University Xi'an 710061 Shaanxi Province China Institute of Infectious Diseases Ditan Hospital 13 Ditan Park Anwai Street Dongcheng District Beijing 100011 China

AIM： To investigate the biological function of HBcAg in pathogenesis of HBV replication in peripheral blood mononuclear cells （PBMCs）.METHODS： HBcAg region was amplified by polymerase chain reaction （PCR） and HBV HBcAg bait plasmid pGBKT7-HBcAg was constructed by routine molecular biological methods. Then the recombinant plasmid DNA was transformed into yeast AH109. After the HBV core protein was expressed in AH109 yeast strains （Western blot analysis）, yeast-two hybrid screening was performed by mating AH109 with Y187 containing leukocyte cDNA library plasmid. Diploid yeast cells were plated on synthetic dropout nutrient medium （SD/-Trp-Leu-His- Ade）（QDO） and synthetic dropout nutrient medium （SD/-Trp-Leu-His-Ade）（TDO）. The second screening was performed with the LacZ report gene （ yeast cells were grown in QDO medium containing X-a-gal）. The interaction between HBV core protein and the protein obtained from positive colonies was further confirmed by repeating yeast-two hybrid. After plasmid DNA was extracted from blue colonies and sequenced, the results were analyzed by bioinformatic methods.RESULTS： Eighteen colonies were obtained and sequenced, including hypermethylated in cancer 2 （3 colones）, eukaryotic translation elongation factor 2 （2 colones）, acetyl-coenzyme A synthetase 3 （1 colone）, DNA polymerase gamma （1 colone）, putative translation initiation factor （1 colone）, chemokine （C-C motif） receptor 5 （1 colone）, mitochondrial ribosomal protein L41 （1 colone）, kyot binding protein genes （1 colone）, RanBPM （1 colone）, HBeAg-binding protein 3 （1 colone）, programmed cell death 2 （1 colone）. Four new genes with unknown function were identified.CONCLUSION： Successful cloning of genes of HBV core protein interacting proteins in leukocytes may provide some new clues for studying the biological functions of HBV core protein.

关键词： Hepatitis B virus Core protein Yeast twohybrid system

Understanding Barriers to Solar Energy Use in Taiwan Using the Decision Making Trial and Evaluation Laboratory Integrated with the Technique for Order Preference by Similarity to an Ideal Solution

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Smart Grid and Renewable Energy 2021年第9期12卷 137-162页

作者： shu-mei lin Graduate Institute of Management of Chang Gung University Taiwan

The aim of this paper was to serve as a reference for the development of renewable energy sources of energy policy in Taiwan by investigating current barriers to solar energy use. Through a meta-analysis of relevant literature, we classified current barriers into 3 dimensions and 13 criteria. Our selected methodology was the Decision Making Trial and Evaluation Laboratory (DE- MATEL) integrated with the Technique for Order Preference by Similarity to an Ideal Solution (TOPSIS). This approach enabled us to determine the relationships among the dimensions and criteria. The results indicate the geographical and topographical factors represent the greatest barriers to solar energy development in Taiwan. Our findings serve as a valuable reference for decision-makers both in terms of policy and investment as well as offer a starting point for those working to priority barriers and choose the optimal barrier to the direction sustainable future in solar energy.

关键词： Energy Renewable Energy Sources Energy Policy DEMATEL and TOPSIS Solar Energy

Screening of hepatocyte proteins binding to F protein of hepatitis C virus by yeast two-hybrid system

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World Journal of Gastroenterology 2005年第36期11卷 5659-5665页

作者： Yan-Ping Huang Jun Cheng shu-lin Zhang lin Wang Jiang Guo Yan Liu Yuan Yang Li-Ying Zhang Gui-Qin Bai Xue-Song Gao Dong ji shu-mei lin Qing Shao The First Hospital of Xi'an Jiaotong University Xi'an 710004 Shaanxi Province China Institute of Infectious Diseases Ditan Hospital Anwai Street Beijing 100011 China

AIM： To investigate the biological function of F protein by yeast two-hybrid system. METHODS： We constructed F protein bait plasmid by cloning the gene of F protein into pGBKT7, then recombinant plasmid DNA was transformed into yeast AH109 （a type）. The transformed yeast AH109 was mated with yeast Y187 （α type） containing liver cDNA library plasmid in 2×YPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium （SD/-Trp-Leu-HisAde） containing X-α-gal for selection and screening. After extracting and sequencing plasmids from positive （blue） colonies, we underwent sequence analysis by bioinformatics. RESULTS： Thirty-six colonies were selected and sequenced. Among them, 11 colonies were zymogen granule protein, 5 colonies were zinc finger protein, 4 colonies were zinc-α-2-glycoprotein, 1 colony was sialyltransferase, 1 colony was complement control protein factor I, 1 colony was vitronectin, and 2 colonies were new genes with unknown function. CONCLUSION： The yeast two-hybrid system is an effective method for identifying hepatocyte proteins interacting with F protein of hepatitis C virus. F protein may bind to different proteins. 2005 The WJG Press and Elsevier Inc. All rights reserved

关键词： Hepatitis C virus F protein Yeast two-hybridsystem

Restoring the Treg cell to Th17 cell ratio may alleviate HBV-related acute-on-chronic liver failure

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World Journal of Gastroenterology 2013年第26期19卷 4146-4154页

作者： Ying-Hua Niu Dong-lin Yin Hong-Li Liu Rui-Tian Yi Yu-Cong Yang Hong-An Xue Tian-Yan Chen shu-lin Zhang shu-mei lin Ying-Ren Zhao Department of Infectious Diseases First Affiliated Hospital of Medical College Xi'an Jiaotong University Department of Infectious Diseases No. 3 People's Hospital Affiliated to School of Medicine Shanghai Jiaotong University Molecular Medicine Center First Affiliated Hospital of Medical College Xi'an Jiaotong University Department of Infectious Diseases Second Affiliated Hospital of Medical College Xi'an Jiaotong University

AIM: To investigate the role of T helper 17 cells (Th17) and regulatory T cells (Treg) in hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF).METHODS: We enrolled 79 patients with HBV infection into the study, 50 patients with HBV-related ACLF and 29 patients with chronic hepatitis B (CHB), from the First Affiliated Hospital of Medical College from January 2009 to June 2012. The ACLF patients were diagnosed according to the criteria recommended by The 19th Conference of the Asian Pacific Association for the Study of the Liver in 2009. Twenty healthy individuals with a similar gender and age structures to the two patient groups were also included as the normal controls (NC). Of the 50 ACLF patients, 28 were subsequently classified as non-survivors: 19 patients died from multiorgan failure, 3 underwent liver transplantation, and 6 discontinued therapy during follow-up because of financial reasons. The remaining 22 ACLF patients whose liver and anticoagulation function recovered to nearly normal levels within the next 6 mo were classified as survivors. The number of circulating Treg and Th17 cells was determined upon diagnosis and during the 8th week of follow-up through flow cytometry. RESULTS: The percentage of circulating Treg cells in the ACLF group was significantly higher than that in the CHB group (5.50% ± 1.15% vs 3.30% ± 1.13%, P < 0.01). The percentages of circulating Th17 cells in the ACLF and the CHB groups were significantly higher than that in the NC group (6.32% ± 2.22% vs 1.56% ± 0.44%, P < 0.01; 3.53% ± 1.65% vs 1.56% ± 0.44%, P < 0.01). No significant difference in Treg cell to Th17 cell ratio was observed between the ACLF group and the CHB group (0.98 ± 0.44 vs 1.12 ± 0.64, P = 0.991), whereas those in the two HBV infection groups were significantly lower than that in the NC group (1.85 ± 1.22; both P < 0.01). The percentage of Treg cells in the survivors during the 8th week of follow-up was significantly lower than that during peak

关键词： Hepatitis B virus Acute-on-chronic liver failure Regulatory T cells T helper 17 cells Treg cell to Th17 cell ratio

Screening of genes of proteins interacting with p7 protein of hepatitis C virus from human liver cDNA library by yeast two-hybrid system

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World Journal of Gastroenterology 2005年第30期11卷 4709-4714页

作者： Yan-Ping Huang shu-lin Zhang Jun Cheng lin Wang Jiang Guo Yan Liu Yuan Yang Li-Ying Zhang Gui-Qin Bai Xue Song Gao Dong Ji shu-mei lin Yan-Wei Zhong Qing Shao The First Hospital of Xi'an Jiaotong University Xi'an 710061 Shaanxi Province China Institute of Infectious Diseases Ditan Hospital Anwai Street Beijing 100011 China

AIM： To investigate the biological function of p7 protein and to look for proteins interacting with p7 protein in hepatocytes. METHODS： We constructed p7 protein bait plasmid by cloning the gene of p7 protein into pGBKTT, then transformed it into yeast AH109 （a type）. The transformed yeast was mated with yeast Y187 （α type） containing liver cDNA library plasmid, pACT2 in 2×YPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium （SD/- Trp-Leu-His-Ade） containing x-α-gal for selection and screening. After extracting and sequencing of plasmids from blue colonies, we performed sequence analysis by bioinformatics. RESULTS： Fifty colonies were selected and sequenced. Among them, one colony was Homo sapiens signal sequence receptor, seven colonies were Homo sapiens H19, seven colonies were immunoglobulin superfamily containing leucine-rich repeat, three colonies were spermatid peri-nuclear RNA binding proteins, two colonies were membrane-spanning 4-domains, 24 colonies were cancer-associated antigens, four colonies were nudeoporin 214 ku and two colonies were CLL-associated antigens. CONCLUSION： The successful cloning of gene of protein interacting with p7 protein paves a way for the study of the physiological function of p7 protein and its assodated protein.

关键词： Hepatitis C virus p7 protein Interacting proteins Yeast two-hybrid system

SYNTHESIS AND PROPERTIES OF NEW INTERPOLYMERIC COMPLEXES

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Chinese Chemical Letters 1993年第5期4卷 387-390页

作者： shu mei DENG, Jing lin, Xu ZHANG Department of Chemistry, Shandong University, Jinan, 250100

β-(2-imidazolinyl)-ethyl methyl diethoxy silane quarternary halides and its condensed polymers with bisphenol A were synthesized and characterized. The polycation-polyanion complexes were prepared by the condensed polymers and poly (styrene sulfonate). The solubility and thermal stability of interpolymeric complexes were investigated.

关键词： SYNTHESIS AND PROPERTIES OF NEW INTERPOLYMERIC COMPLEXES Wang

Clinical characteristics and current management of hepatitis B and C in China

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World Journal of Gastroenterology 2014年第37期20卷 13582-13590页

作者： Yong-Tao Sun Yue-Xin Zhang Hong Tang Qing Mao Xiao-Zhong Wang ling-Yi Zhang Hong Chen Ying-Na Zhong shu-mei lin Da-Zhi Zhang Department of Infectious Diseases Tangdu HospitalAffiliated to the Fourth Military Medical University the First Affiliated Hospital of Xinjiang Medical University West China Hospital Sichuan University the First Affiliated Hospital of the Third Military Medical University Traditional Chinese Medicine Hospital of Xinjiang Uygur Administrative Region the Second Affiliated Hospital of Lanzhou University the First Affiliated Hospital of Lanzhou University People’s Hospital of Xinjiang Uygur Administrative Region the First Affiliated Hospital of Xi'an Jiaotong University the Second Affiliated Hospital of Chongqing Medica University

AIM: To describe a population of outpatients in China infected by hepatitis B virus (HBV) and/or hepatitis C virus (HCV), and assess their current management status. METHODS: A multicenter, cross-sectional study of HBV- and/or HCV-infected patients was conducted from August to November, 2011 in western China. Patients >= 18 years of age with HBV and/or HCV infections who visited outpatient departments at 10 hospitals were evaluated, whether treated or not. Data were collected on the day of visit from medical records and patient interviews. RESULTS: A total 4010 outpatients were analyzed, including 2562 HBV- infected and 1406 HCV-infected and 42 HBV/HCV co-infected patients. The median duration of documented infection was 7.5 years in HBV- infected and 1.8 years in HCV-infected patients. Cirrhosis was the most frequent hepatic complication (12.2%), appearing in one-third of patients within 3 years prior to or at diagnosis. The HCV genotype was determined in only 10% of HCV-infected patients. Biopsy data were only available for 54 patients (1.3%). Antiviral medications had been received by 58.2% of patients with HBV infection and 66.6% with HCV infection. Nucleos(t) ide analogs were the major antiviral medications prescribed for HBV- infected patients (most commonly adefovir dipivoxil and lamivudine). Ribavirin + pegylated interferon was prescribed for two-thirds of HCV-infected patients. In the previous 12 mo, around one-fifth patients had been hospitalized due to HBV or HCV infection. CONCLUSION: This observational, real-life study has identified some gaps between clinical practice and guideline recommendations in China. To achieve better health outcomes, several improvements, such as disease monitoring and optimizing antiviral regimens, should be made to improve disease management. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.

关键词： Hepatitis B Hepatitis C Clinical characteristics Treatment

Calcitriol attenuates liver fibrosis through hepatitis C virus nonstructural protein 3-transactivated protein 1-mediated TGF β1/Smad3 and NF-κB signaling pathways

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World Journal of Gastroenterology 2023年第18期29卷 2798-2817页

作者： Liu Shi Li Zhou Ming Han Yu Zhang Yang Zhang Xiao-Xue Yuan Hong-Ping Lu Yun Wang Xue-Liang Yang Chen Liu Jun Wang Pu Liang shun-Ai Liu Xiao-Jing Liu Jun Cheng shu-mei lin Department of Infectious Disease Medicine The First Hospital Affiliated to Xi’an Jiaotong UniversityXi’an 710061Shaanxi ProvinceChina China-Japan Friendship Hospital Department of Infectious Disease China-Japan Friendship HospitalBeijing 100029China Institute of Infectious Diseases Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China The Division of Liver Diseases Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China Institute of Liver Diseases Beijing Pan-Asia Tongze Institute of Biomedicine Co.LtdBeijing 100015China Department of Rehabilitation Medicine The First Affiliated Hospital of Xi’an Jiaotong UniversityXi’an 710061Shaanxi ProvinceChina Beijing Key Laboratory of Emerging Infectious Diseases Peking University Ditan Teaching HospitalBeijing 100015China

BACKGROUND Hepatic fibrosis is a serious condition,and the development of hepatic fibrosis can lead to a series of ***,the pathogenesis of hepatic fibrosis remains unclear,and effective therapy options are still *** group identified hepatitis C virus nonstructural protein 3-transactivated protein 1(NS3TP1) by suppressive subtractive hybridization and bioinformatics analysis,but its role in diseases including hepatic fibrosis remains ***,additional studies on the function of NS3TP1 in hepatic fibrosis are urgently needed to provide new targets for *** To elucidate the mechanism of NS3TP1 in hepatic fibrosis and the regulatory effects of calcitriol on *** Twenty-four male C57BL/6 mice were randomized and separated into three groups,comprising the normal,fibrosis,and calcitriol treatment groups,and liver fibrosis was modeled by carbon tetrachloride(CCl4).To evaluate the level of hepatic fibrosis in every group,serological and pathological examinations of the liver were ***-β1 was administered to boost the in vitro cultivation of LX-2 ***3TP1,α-smooth muscle actin(α-SMA),collagen I,and collagen Ⅲ in every group were examined using a Western blot and real-time quantitative polymerase chain *** activity of the transforming growth factor beta 1(TGFβ1)/Smad3 and NF-κB signaling pathways in each group of cells transfected with pcDNA-NS3TP1 or siRNA-NS3TP1 was *** statistical analysis of the data was performed using the Student’s t *** NS3TP1 promoted the activation,proliferation,and differentiation of hepatic stellate cells(HSCs)and enhanced hepatic fibrosis via the TGFβ1/Smad3 and NF-κB signaling pathways,as evidenced by the presence of α-SMA,collagen I,collagen Ⅲ,p-smad3,and p-p65 in LX-2 cells,which were upregulated after NS3TP1 overexpression and downregulated after NS3TP1 *** proliferation of HSCs was lowered after NS3TP1 interference and elevated after

关键词： Nonstructural protein 3-transactivated protein 1 Calcitriol Liver fibrosis Hepatic stellate cells Mouse model TGFβ1/Smad3 NF-κB Signaling pathway

Plasma exchange-centered artificial liver support system in hepatitis B virus-related acute-onchronic liver failure:a nationwide prospective multicenter study in China

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Hepatobiliary & Pancreatic Diseases International 2016年第3期15卷 275-281页

作者： Jia-Jia Chen Jian-Rong Huang Qian Yang Xiao-Wei Xu Xiao-Li Liu Shao-Rui Hao Hui-Fen Wang Tao Han Jing Zhang Jian-He Gan Zhi-Liang Gao Yu-Ming Wang shu-mei lin Qing Xie Chen Pan Lan-Juan Li State Key Laboratory for Diagnosis and Treatment of Infectious Diseases and Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesFirst Affiliated HospitalZhejiang University School of Medicine Liver Failure Therapy and Research Center 302 Hospital of People’s Liberation Army Department of Hepatology Third Central Clinical College of Tianjin Medical University Artificial Liver Center You’an HospitalCapital Medical University Department of Infectious Diseases First Affiliated Hospital of Suzhou University Department of Infectious Diseases Third Affiliated Hospital of Sun Yat-Sen University Institute of Infectious Diseases Southwest HospitalThird Military Medical University Department of Infectious Diseases First Affiliated Hospital of Xi’an Jiaotong University Department of Infectious Diseases Ruijin HospitalShanghai Jiaotong University School of Medicine Department of Infectious Diseases Infectious Diseases Hospital Affiliated to Fujian Medical University

BACKGROUND： Plasma exchange （PE）-centered artificial liver support system reduced the high mortality rate of hepa titis B virus （HBV）-related acute-on-chronic liver failure （ACLF）. But the data were diverse in different medical centers. The present prospective nationwide study was to evaluate the effects of PE on patients with HBV-ACLF at different stages.

关键词： liver failure artificial liver support plasma exchange acute-on-chronic liver failure