Screening of hepatocyte proteins binding to F protein of hepatitis C virus by yeast two-hybrid system
Screening of hepatocyte proteins binding to F protein of hepatitis C virus by yeast two-hybrid system作者机构：The First Hospital of Xi'an Jiaotong University Xi'an 710004 Shaanxi Province China Institute of Infectious Diseases Ditan Hospital Anwai Street Beijing 100011 China
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2005年第11卷第36期
页 面：5659-5665页
核心收录：
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学]
基 金：Supported by the National Natural Science Foundation of China Nos. C03011402 and C30070690 and the Research and Technique Foundation of PLA during the 9~(th)-Five year plan period No. 98D063 and the Launching Foundation for Student Studying Abroad of PLA No. 98H038 and the Youth Research and Technique Foundation of PLA during the 10~(th)-Five Year Plan Period No. 01Q138 and the Research and Technique Foundation of PLA during the 10~(th)-Five Year Plan Period No. 01MB135
主 题：Hepatitis C virus F protein Yeast two-hybridsystem
摘 要：AIM： To investigate the biological function of F protein by yeast two-hybrid system. METHODS： We constructed F protein bait plasmid by cloning the gene of F protein into pGBKT7, then recombinant plasmid DNA was transformed into yeast AH109 （a type）. The transformed yeast AH109 was mated with yeast Y187 （α type） containing liver cDNA library plasmid in 2×YPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium （SD/-Trp-Leu-HisAde） containing X-α-gal for selection and screening. After extracting and sequencing plasmids from positive （blue） colonies, we underwent sequence analysis by bioinformatics. RESULTS： Thirty-six colonies were selected and sequenced. Among them, 11 colonies were zymogen granule protein, 5 colonies were zinc finger protein, 4 colonies were zinc-α-2-glycoprotein, 1 colony was sialyltransferase, 1 colony was complement control protein factor I, 1 colony was vitronectin, and 2 colonies were new genes with unknown function. CONCLUSION： The yeast two-hybrid system is an effective method for identifying hepatocyte proteins interacting with F protein of hepatitis C virus. F protein may bind to different proteins. 2005 The WJG Press and Elsevier Inc. All rights reserved
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