Calcitriol attenuates liver fibrosis through hepatitis C virus nonstructural protein 3-transactivated protein 1-mediated TGF β1/Smad3 and NF-κB signaling pathways

作     者：Liu Shi Li Zhou Ming Han Yu Zhang Yang Zhang Xiao-Xue Yuan Hong-Ping Lu Yun Wang Xue-Liang Yang Chen Liu Jun Wang Pu Liang Shun-Ai Liu Xiao-Jing Liu Jun Cheng Shu-Mei Lin 

作者机构：Department of Infectious Disease MedicineThe First Hospital Affiliated to Xi’an Jiaotong UniversityXi’an 710061Shaanxi ProvinceChina China-Japan Friendship HospitalDepartment of Infectious Disease China-Japan Friendship HospitalBeijing 100029China Institute of Infectious DiseasesBeijing Ditan HospitalCapital Medical UniversityBeijing 100015China The Division of Liver DiseasesBeijing Ditan HospitalCapital Medical UniversityBeijing 100015China Institute of Liver DiseasesBeijing Pan-Asia Tongze Institute of Biomedicine Co.LtdBeijing 100015China Department of Rehabilitation MedicineThe First Affiliated Hospital of Xi’an Jiaotong UniversityXi’an 710061Shaanxi ProvinceChina Beijing Key Laboratory of Emerging Infectious DiseasesPeking University Ditan Teaching HospitalBeijing 100015China 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2023年第29卷第18期

页      面：2798-2817页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学] 

基　　金：the National Key Research and Development Program of China,No.2017YFC0908104 National Science and Technology Projects,No.2017ZX10203201,No.2017ZX10201201,and No.2017ZX10202202 

主　　题：Nonstructural protein 3-transactivated protein 1 Calcitriol Liver fibrosis Hepatic stellate cells Mouse model TGFβ1/Smad3 NF-κB Signaling pathway 

摘      要：BACKGROUND Hepatic fibrosis is a serious condition,and the development of hepatic fibrosis can lead to a series of ***,the pathogenesis of hepatic fibrosis remains unclear,and effective therapy options are still *** group identified hepatitis C virus nonstructural protein 3-transactivated protein 1(NS3TP1) by suppressive subtractive hybridization and bioinformatics analysis,but its role in diseases including hepatic fibrosis remains ***,additional studies on the function of NS3TP1 in hepatic fibrosis are urgently needed to provide new targets for *** To elucidate the mechanism of NS3TP1 in hepatic fibrosis and the regulatory effects of calcitriol on *** Twenty-four male C57BL/6 mice were randomized and separated into three groups,comprising the normal,fibrosis,and calcitriol treatment groups,and liver fibrosis was modeled by carbon tetrachloride(CCl4).To evaluate the level of hepatic fibrosis in every group,serological and pathological examinations of the liver were ***-β1 was administered to boost the in vitro cultivation of LX-2 ***3TP1,α-smooth muscle actin(α-SMA),collagen I,and collagen Ⅲ in every group were examined using a Western blot and real-time quantitative polymerase chain *** activity of the transforming growth factor beta 1(TGFβ1)/Smad3 and NF-κB signaling pathways in each group of cells transfected with pcDNA-NS3TP1 or siRNA-NS3TP1 was *** statistical analysis of the data was performed using the Student’s t *** NS3TP1 promoted the activation,proliferation,and differentiation of hepatic stellate cells(HSCs)and enhanced hepatic fibrosis via the TGFβ1/Smad3 and NF-κB signaling pathways,as evidenced by the presence of α-SMA,collagen I,collagen Ⅲ,p-smad3,and p-p65 in LX-2 cells,which were upregulated after NS3TP1 overexpression and downregulated after NS3TP1 *** proliferation of HSCs was lowered after NS3TP1 interference and elevated after