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Different Strategies for Preparation of Non-tagged rV270 Protein and Its Efficacy against Yersinia Pestis Challenge

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Biomedical and Environmental Sciences 2010年第5期23卷 333-340页

作者： WANG WANG ZHI-ZHEN QI QING-WEN ZHANG BEN-CHUAN WU ZI-WEN ZHU YONG-HAI yang BAI-ZHONG CUI rui-XIA DAI YE-FENG QIU ZU-YUN WANG ZHAO-BIAO GUO TAO-XING SHI HU WANG rui-fu yang XIAO-YI WANG Laboratory of Analytical Microbiology State Key Laboratory of Pathogen and BiosecurityBeijing Institute of icrobiology and EpidemiologyBeijing 100071China Qinghai Institute for Endemic Disease Prevention and Control of Qinghai Province Xining811602China Beijing Institute of Bioengneering Beijing 100071China

Objective LcrV is an important component for the development of a subunit vaccine against *** reduce immunosuppressive activity of LcrV,a recombinant LcrV variant lacking amino acids 271 to 326 （rV270） was prepared by different methods in this *** A new strategy that produced non-tagged or authentic rV270 protein was designed by insertion of rV270-thrombin-hexahistidine fusion gene into the vector pET24a,or by insertion of hexahistidine-enterokinase-rV270 or hexahistitine-factor Xa-rV270 fusion gene into the vector *** Co2＋ affinity chromatography,a purification strategy was developed by cleavage of His tag on column,following Sephacryl S-200HR column filtration *** Removal of His tag by thrombin,enterokinase and factor Xa displayed a yield of 99.5%,32.4% and 15.3%,*** Sephacryl S-200HR column filtration chromatography,above 97% purity of rV270 protein was *** rV270 that was adsorbed to 25% （v/v） Al（OH）3 adjuvant in phosphate-buffered saline （PBS） induced very high titers of antibody to rV270 in BALB/c mice and protected them （100% survival） against subcutaneous challenge with 106 Cfu of *** virulent strain *** The completely authentic rV270 protein can be prepared by using enterokinase or factor Xa,but they exhibited extremely low cleavage activity to the corresponding recognition *** cleavage is an efficient strategy to prepare non-tagged rV270 protein and can be easily operated in a large scale due to its relatively low cost and high cleavage *** recombinant rV270 can be used as a key component to develop a subunit vaccine of plague.

关键词： Yersinia pestis rV270 antigen Purification Protection Plague

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二期梅毒误诊为银屑病2例

中国皮肤性病学杂志 2017年第1期31卷 104-105页

作者：付锐杨钧济宁市皮肤病防治院山东济宁272000

例1女,16岁。全身红斑并鳞屑1个月。例2男,35岁,躯干和四肢红斑2周。2例均被误诊为银屑病。TRUST 1∶32(+),TPPA(+)。例1皮损组织病理示:真皮浅层较多浆细胞及淋巴细胞浸润。诊断:二期梅毒。均予苄星青霉素240万U分两侧臀部肌注,1次/周,... 详细信息

例1女,16岁。全身红斑并鳞屑1个月。例2男,35岁,躯干和四肢红斑2周。2例均被误诊为银屑病。TRUST 1∶32(+),TPPA(+)。例1皮损组织病理示:真皮浅层较多浆细胞及淋巴细胞浸润。诊断:二期梅毒。均予苄星青霉素240万U分两侧臀部肌注,1次/周,3周后皮损消退。现随访中。

关键词：二期梅毒银屑病误诊

Reversing anther thermotolerance by manipulating the cis-elements in the promoter of a high-temperature upregulated gene Casein Kinase I in upland cotton

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Science China Life Sciences 2025年

作者： Yanlong Li Yinuo fu Yaoyao Li rui Zhang Jing yang Huanhuan Ma Ling Min Xianlong Zhang National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University

High temperature (HT) stress causes male sterility,leading to reduced upland cotton ***,we identified a key gene,Casein Kinase I (GhCKI),that negatively regulates male fertility in upland cotton under ***,conventional genetic manipulations of GhCKI would result in male sterility,hindering its utilization in breeding ***,we engineered quantitative variation for anther thermotolerance-related traits in upland cotton by creating weak promoter alleles of GhCKI genes,using CRISPR/Cas9 and CRISPR/Cpf1genome ***,we screened and identified two new upland cotton plant lines exhibiting a HT-tolerant phenotype with edited GhCKI promoters,and characterized their corresponding heat-tolerant allelic *** research revealed that the primary reason for the HT tolerance of the GhCKI promoter editing mutants is that the trans-acting factors GhMYB73 and GhMYB4,which positively regulate GhCKI expression under HT,failed to bind and activate the expression of ***,our study not only provides a rapid strategy to generate new beneficial alleles but also offers novel germplasm resources and molecular insights for crop HT tolerance breeding.

关键词：

In silico assessment of the impact of 2019 novel coronavirus genomic variation on the efficiency of published real-time quantitative polymerase chain reaction detection assays

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Chinese Medical Journal 2020年第13期133卷 1612-1613页

作者： Hang Fan Xiang-Li-Lan Zhang Ya-Wei Zhang Yong Huang Yue Teng Yan Guo Zhi-Qiang Mi rui-fu yang Ya-Jun Song Yu-Jun Cui State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing 100071China

To the Editor:In December 2019,coronavirus disease 2019 outbreak caused by the 2019 novel coronavirus(2019-nCoV)happened in Wuhan,***,it has posed a worldwide public health ***-time quanti-tative polymerase chain reaction(RT-qPCR)was recom-mended as an effective pathogen detection method and has played an important role in prevention and control of the current *** research institutions have released their primer sets for *** the variant sites were located in the primer regions,the efficiency of RT-qPCR would be reduced,thus possibly causing false negative results,and leading to unpredictable impact on the diagnosis of patients and the control of this ***,a comprehensive investigation on 2019-nCoV genome variation is necessary to evaluate the effectiveness of current released RT-qPCR methods.

关键词： prevention diagnosis impact

Identification of an epitope of SARS-coronavirus nucleocapsid protein

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Cell Research 2003年第3期13卷 141-145页

作者： YING LIN,XU SHEN,rui fu yang,YI XUE LI,YONG YONG JI,YOU Yu HE,MU DE SHI,WEI LU,TIE LIU SHI,JIN WANG,HONG XIA WANG,HUA LIANG JIANG,JIAN HUA SHEN,You HUA XIE,YUAN WANG,GANG PEI,BEI FEN SHEN,JIA rui WU,BING SUNInstitute of Biochemistry and Cell Biology,Institute of Materia Medico,,Bioinformation Center,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences. Shanghai 200031,ChinaInstitute of Microbiology and Epidemiology,Institute of Basic Medical Sciences,Academy of Military Medical,Sc.imce.fi,Beijing 100071,China Institute of Biochemistry and Cell Biology Chinese Academy of Sciences Shanghai 200031 China.

The nucleocapsid(N)protein of severe acute respiratory syndrome-coronavirus(SARS-CoV)is a majorvirion structural *** this study,two epitopes(N1 and N2)of the N protein of SARS-CoV werepredicted by bioinformatics *** immnnization with two peptides,the peptides-specific antibodieswere isolated from the immunized *** further experiments demonstrated that N1 peptide-inducedpolyclonal antibodies had a high affinity to bind to *** expressed Nprotein of ***,itwas confirmed that N1 peptide-specific IgG antibodies were detectable in the sera of severe acute respiratorysyndrome(SARS)*** results indicated that an epitope of the N protein has been identified andN protein specific Abs were produced by peptide immunization,which will be useful for the study of SARS-CoV.

关键词： severe acute respiratory syndrome-coronavirus necleocapsid protein epitope polyclonal antibody antiserum.

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论翻译多项选择题测试的有效性

杨凌职业技术学院学报 2009年第4期8卷 95-97页

作者：李凯付瑞阳杨凌职业技术学院陕西杨凌712100

翻译多项选择题是翻译测试题型的一种尝试。本文结合测试目的与题型特点从题型设计的角度和实际运用的效果出发对翻译多项选择题的有效性进行科学论证,分析了不足之处,找出了存在的问题和今后的研究方向。

关键词：翻译测试多项选择题有效性信度效度

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英语教学中如何培养学生的跨文化意识

杨凌职业技术学院学报 2009年第4期8卷 82-84页

作者：付瑞阳李凯杨凌职业技术学院陕西杨凌712100

成功有效的英语教学应该改变单一的"为语言而学语言"的传统课堂教学模式,应向语言知识教学与文化内容传授相结合的方向转变。英语教师要重视文化导入,在教学中注重培养学生的跨文化意识。

关键词：英语教学跨文化意识教学模式

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疏利少阳针刺法治疗颈性眩晕65例

中国针灸 2023年第12期43卷 1431-1432页

作者：卢晓茵沈菲赵增趁高珺傅瑞阳浙江中医药大学附属湖州中医院针推康复科湖州313000 解放军总医院第八医学中心

颈性眩晕是以头晕目眩甚则恶心呕吐为主要临床表现的综合征。成年人颈性眩晕的发病率逐渐增高,以中老年居多,并日趋年轻化[1]。西医以血管扩张剂等药物治疗为主,具有起效慢、疗程长、复发率较高等不足[2]。而针刺治疗颈性眩晕已有明确疗... 详细信息

颈性眩晕是以头晕目眩甚则恶心呕吐为主要临床表现的综合征。成年人颈性眩晕的发病率逐渐增高,以中老年居多,并日趋年轻化[1]。西医以血管扩张剂等药物治疗为主,具有起效慢、疗程长、复发率较高等不足[2]。而针刺治疗颈性眩晕已有明确疗效[3-4]。笔者采用疏利少阳针刺法治疗颈性眩晕65例,现报道如下。

关键词：颈性眩晕针刺疏利少阳神经肽Y 血管活性因子

Genotyping of strains of Yersinia pestis from Marmota baibacina-Spermophilus undulates Plague Focus of Tianshan Mountains in China

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Journal of Microbiology and Immunology 2005年第4期3卷 266-269页

作者： LI XIA JIN ER HEI DAI DONG SHENG ZHOU BAI ZHONG CWI RI XIA DAI SHAN LONG YUE MEI YING QI HAI HONG ZHAO CUN XIANG LI XIAO YAN yang XIAO TAO YU XIANG DAI MIN LI rui fu yang Qinghai Institute for Endemic Diseases Control and Research Xining P. R. China Instttue of Microbiology and Epidemiology Academy of Military Medical Scicences Belting P. R China 3Xinjiang Provincial Center for Dieases Control and Prevention Wulumuqi P. R. China

The aim of this study is to carry out genotyping of 61 Y. pestts strmns tsolated trom lwarmota baibacina-Spermophilus undulates Plague Focus of Tianshan Mountains in China. Primer pairs targeting the 22 DFRs were designed for detecting the genotypes of 61 strains. As a result, 61 strains of Y. pestis were divided into four genotypes 1, 2, 3, and 4. Genotypes 1, 2 and 3 were found from west part in Northern Tianshan Mountains Plague Focus, but type 1 was only from Nileke county. The type of strains from Aheqi was different from those of Atushi counties in Southern Tianshan Mountains and similar to strains in Northern Tianshan Mountains Plague Focus. The type 4 distributed over Atushi county, which was identical with that of strains from Marmota caudae Plague Focus of the Pamiri Plateau. It is concluded that geonotyping is identical with ecotyping made by Shuli Ji. Tianshan Mountains Plague Focus and Marmota caudae Plague Focus of the Pamiri Plateau have a cross spreading profile.

关键词： Yersinia pestis PCR Comparative genomics DFR Plague Focus Natural