In silico assessment of the impact of 2019 novel coronavirus genomic variation on the efficiency of published real-time quantitative polymerase chain reaction detection assays

作     者：Hang Fan Xiang-Li-Lan Zhang Ya-Wei Zhang Yong Huang Yue Teng Yan Guo Zhi-Qiang Mi Rui-Fu Yang Ya-Jun Song Yu-Jun Cui Fan Hang;Zhang Xiang-Li-Lan;Zhang Ya-Wei;Huang Yong;Teng Yue;Guo Yan;Mi Zhi-Qiang;Yang Rui-Fu;Song Ya-Jun;Cui Yu-Jun

作者机构：State Key Laboratory of Pathogen and BiosecurityBeijing Institute of Microbiology and EpidemiologyBeijing 100071China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2020年第133卷第13期

页      面：1612-1613页

核心收录：

学科分类：100208[医学-临床检验诊断学] 1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：This work was supported by the National Key Research&Development Program of China(No.2020YFC0840900) the Beijing Municipal Science and Technology Project(No.Z201100001020004) 

主　　题：prevention diagnosis impact 

摘      要：To the Editor:In December 2019,coronavirus disease 2019 outbreak caused by the 2019 novel coronavirus(2019-nCoV)happened in Wuhan,***,it has posed a worldwide public health ***-time quanti-tative polymerase chain reaction(RT-qPCR)was recom-mended as an effective pathogen detection method and has played an important role in prevention and control of the current *** research institutions have released their primer sets for *** the variant sites were located in the primer regions,the efficiency of RT-qPCR would be reduced,thus possibly causing false negative results,and leading to unpredictable impact on the diagnosis of patients and the control of this ***,a comprehensive investigation on 2019-nCoV genome variation is necessary to evaluate the effectiveness of current released RT-qPCR methods.