Identification of an epitope of SARS-coronavirus nucleocapsid protein

作     者：YING LIN,XU SHEN,RUI FU YANG,YI XUE LI,YONG YONG JI,YOU Yu HE,MU DE SHI,WEI LU,TIE LIU SHI,JIN WANG,HONG XIA WANG,HUA LIANG JIANG,JIAN HUA SHEN,You HUA XIE,YUAN WANG,GANG PEI,BEI FEN SHEN,JIA RUI WU,BING SUNInstitute of Biochemistry and Cell Biology,Institute of Materia Medico,,Bioinformation Center,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences. Shanghai 200031,ChinaInstitute of Microbiology and Epidemiology,Institute of Basic Medical Sciences,Academy of Military Medical,Sc.imce.fi,Beijing 100071,China 

作者机构：Institute of Biochemistry and Cell Biology Chinese Academy of Sciences Shanghai 200031 China. 

出 版 物：《Cell Research》 (细胞研究(英文版))

年 卷 期：2003年第13卷第3期

页      面：141-145页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：Technology Committee(03DZ19113) 国家重点基础研究发展计划(973计划)(2001CB510006) 国家高技术研究发展计划(863计划)(2001AA231011) specific project against SARS from Chinese Academy of Sciences 

主　　题：severe acute respiratory syndrome-coronavirus necleocapsid protein epitope polyclonal antibody antiserum. 

摘      要：The nucleocapsid(N)protein of severe acute respiratory syndrome-coronavirus(SARS-CoV)is a majorvirion structural *** this study,two epitopes(N1 and N2)of the N protein of SARS-CoV werepredicted by bioinformatics *** immnnization with two peptides,the peptides-specific antibodieswere isolated from the immunized *** further experiments demonstrated that N1 peptide-inducedpolyclonal antibodies had a high affinity to bind to *** expressed Nprotein of ***,itwas confirmed that N1 peptide-specific IgG antibodies were detectable in the sera of severe acute respiratorysyndrome(SARS)*** results indicated that an epitope of the N protein has been identified andN protein specific Abs were produced by peptide immunization,which will be useful for the study of SARS-CoV.