Reversing anther thermotolerance by manipulating the cis-elements in the promoter of a high-temperature upregulated gene Casein Kinase I in upland cotton
作者机构：National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural University
出 版 物：《Science China Life Sciences》 (中国科学:生命科学(英文版))
年 卷 期：2025年
核心收录：
基 金：supported by Biological Breeding of Stress tolerant and High Yield Cotton Varieties(2023ZD040403) Postdoctoral Fellowship Program of CPSF (GZB20230247,2024M751039) Science Fund for Distinguished Young Scholars of Hubei Province (2024AFA059)
摘 要：High temperature (HT) stress causes male sterility,leading to reduced upland cotton ***,we identified a key gene,Casein Kinase I (GhCKI),that negatively regulates male fertility in upland cotton under ***,conventional genetic manipulations of GhCKI would result in male sterility,hindering its utilization in breeding ***,we engineered quantitative variation for anther thermotolerance-related traits in upland cotton by creating weak promoter alleles of GhCKI genes,using CRISPR/Cas9 and CRISPR/Cpf1genome ***,we screened and identified two new upland cotton plant lines exhibiting a HT-tolerant phenotype with edited GhCKI promoters,and characterized their corresponding heat-tolerant allelic *** research revealed that the primary reason for the HT tolerance of the GhCKI promoter editing mutants is that the trans-acting factors GhMYB73 and GhMYB4,which positively regulate GhCKI expression under HT,failed to bind and activate the expression of ***,our study not only provides a rapid strategy to generate new beneficial alleles but also offers novel germplasm resources and molecular insights for crop HT tolerance breeding.
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