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Variation and stability analysis of growth traits of poplar clones in the seedling stage in northeast China

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Journal of Forestry Research 2023年第4期34卷 1107-1116页

作者： Xiaoting Liu qiushuang Zhao Peng Yin Hanxi Li Xuesong Li Liguo Wu Yueji Li Yanbo Hu Xiyang Zhao State Key Laboratory of Tree Genetics and Breeding Northeast Forestry UniversityHarbin 150040People’s Republic of China College of Forestry and Grasslands Jilin Agricultural UniversityChangchun 130118People’s Republic of China Dongling State-Owned Forest Protection Center Songyuan 138000People’s Republic of China Cuohai Forest Farm of Longjiang Qiqihar 161000People’s Republic of China Baicheng State-Owned Forest Farm Baicheng 137000People’s Republic of China

Plant phenotypes are infl uenced by genetic and environmental *** this study,the growth traits of 43 one-year-old poplar clones grown at diff erent sites in northeast China(Dongling State-owned Forest Protection Center,DL;Baicheng State-owned forest farm,BC;and Cuohai Forest farm,CH)were evaluated and analyzed across clones and *** show signifi cant diff erences in height and base diameter among sites and *** and genetic variation coeffi cients ranged from 49.59%(BC)to 58.39%(DL)and from 49.33%(BC)to 58.06%(DL),*** main eff ects and multiplicative interaction(AMMI)analysis showed that the eff ects of genotype,environment,and genotype×environment interaction were signifi cantly diff *** variation was the main source of variation,accounting for 48.6%.AMMI biplot showed that clone 30 had high and stable yields at the three *** an evaluation of multiple traits and GGE biplot that clone 2,clone 30 and clone 25 had higher yield than the other clones at DL,CH and BC,*** clones will provide material for forest regeneration in northeast China.

关键词： Populus Genetic variation Genotype×environment interaction AMMI model GGE biplot

A Combinatorial Approach Based on Nucleic Acid Assembly and Electrostatic Compression for siRNA Delivery

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Chemical Research in Chinese Universities 2021年第4期37卷 906-913页

作者： REN Yiqing LIU Xinlong GE Huan GUO Yuanyuan ZHANG qiushuang XIE Miao WANG Ping ZHU Xinyuan ZHANG Chuan School of Chemistry and Chemical Engineering Frontiers Science Center for Transformative MoleculesShanghai Jiao Tong UniversityShanghai200240P.R.China Weigao Research Center Shanghai201203P.R.China

A combinatorial strategy based on nucleic acid assembly and electrostatic complexation is developed for efficient small interfering ribonucleic acid(siRNA)*** this approach,siRNAs are first loaded into a well-defined nanotube through programmable nucleic acid *** to small rigid siRNA duplex,the obtained siRNA-bearing nanotube with large architecture is more readily to complex with cationic and ionizable poly(β-amino ester),resulting in the formation of a novel platform for efficient siRNA delivery.

关键词： Nucleic acid nanogel Electrostatic compression siRNA delivery Self-assembly

Engineering of triterpene metabolism and overexpression of the lignin biosynthesis gene PAL promotes ginsenoside Rg3 accumulation in ginseng plant chassis

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Journal of Integrative Plant Biology 2022年第9期64卷 1739-1754页

作者： Lu Yao Huanyu Zhang Yirong Liu qiushuang Ji Jing Xie Ru Zhang Luqi Huang Kunrong Mei Juan Wang Wenyuan Gao School of Pharmaceutical Science and Technology Tianjin UniversityTianjin300072 China Wenzhou Safety(Emergency)Institute of Tianjin University Wenzhou325000 China Key Laboratory of Systems Bioengineering Ministry of EducationTianjin UniversityTianjin300072 China National Resource Center for Chinese Meteria Medica China Academy of Chinese Medical SciencesBeijing100700 China

The ginsenoside Rgfound in Panax species has extensive pharmacological properties,in particular anti-cancer ***,its natural yield in Panax plants is ***,we report a multimodular strategy to improve yields of Rgin a Panax ginseng chassis,combining engineering of triterpene metabolism and overexpression of a lignin biosynthesis gene,phenylalanine ammonia lyase(PAL).We first performed semi-rational design and site mutagenesis to improve the enzymatic efficiency of Pq3-O-UGT2,a glycosyltransferase that directly catalyzes the biosynthesis of Rgfrom ***,we used clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)gene editing to knock down the branch pathway of protopanaxatriol-type ginsenoside biosynthesis to enhance the metabolic flux of the protopanaxadiol-type ginsenoside *** of PAL accelerated the formation of the xylem structure,significantly improving ginsenoside Rgaccumulation(to 6.19-fold higher than in thecontrol).Wecombinedoverexpression of the ginsenoside aglycon synthetic genes squalene epoxidase,Pq3-O-UGT2,and PAL with CRISPR/Cas9-based knockdown of CYP716A53v2 to improve ginsenoside ***,we produced ginsenoside Rgat a yield of 83.6 mg/L in a shake flask(7.0 mg/g dry weight,21.12-fold higher than with wild-type cultures).The highproduction system established in this study could be a potential platform to produce the ginsenoside Rgcommercially for pharmaceutical use.

关键词： catalysis ginsenoside Rg3 metabolism regulation phenylalanine ammonia lyase

Combined effects of cadmium and fluoranthene on germination, growth and photosynthesis of soybean seedlings

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Journal of Environmental Sciences 2013年第9期25卷 1936-1946页

作者： qiushuang Li Yonglong Lu Yajuan Shi Tieyu Wang Kun Ni Li Xu Shijie Liu Lin Wang Qinli Xiong John.P Giesy State Key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences University of Chinese Academy of Sciences Department of Veterinary Biomedical Sciences and Toxicology Centre University of Saskatchewan

The single and combinational effects of cadmium （Cd） and fluoranthene （FLT） on germination, growth and photosynthesis of soybean seedlings were investigated. Exposure to 5, 10, or 15 mg Cd/L or 1, 5, or 10 mg FLT/L individually or in combination significantly decreased germination vigor （3 days） and final germination rate of soybean seeds, except at 1 and 5 mg FLT/L. The results of two-way ANOVA analysis and the Bliss independence model showed that at lower concentrations of FLT （1 mg/L）, the interaction between Cd and FLT on germination was antagonistic, whereas the interaction was synergistic when the concentration of FLT was 5 or 10 mg/L and the concentration of Cd was 15 mg/L. Growth, expressed as dry weight, length of shoot and root, leaf area, and photosynthesis, expressed as net photosynthetic rate, intercellular CO2 concentration, chlorophyll contents and fluorescence of soybean seedlings were also reduced by exposure to 5 or 10 mg Cd/L or 1 or 5 mg FLT/L, singly or jointly. Significant antagonistic effects of exposure to 5 or 10 mg Cd/L or 1 or 5 mg FLT/L on shoot growth and photosynthesis were observed, whereas synergy and antagonism of Cd and FLT were both observed for root growth.

关键词： synergism antagonism metal PAH photosynthesis plant toxicity

Genome-wide association analysis identified molecular markers associated with important tea flavor-related metabolites

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Horticulture Research 2021年第1期8卷 714-730页

作者： Kaixing Fang Zhiqiang Xia Hongjian Li Xiaohui Jiang Dandan Qin qiushuang Wang Qing Wang Chendong Pan Bo Li Hualing Wu Tea Research Institute Guangdong Academy of Agricultural SciencesGuangdong Key Laboratory of Tea Plant Resources Innovation&UtilizationGuangzhou 510640China Institute of Biotechnology Chinese Academy of Tropical Agricultural SciencesHaikou 570100China Hainan University Haikou 570228China

The characteristic secondary metabolites in tea(theanine,caffeine,and catechins)are important factors contributing to unique tea ***,there has been relatively little research on molecular markers related to these ***,we conducted a genome-wide association analysis of the levels of these tea flavor-related metabolites in three *** theanine,caffeine,and catechin levels in Population 1 comprising 191 tea plant germplasms were examined,which revealed that their heritability exceeded 0.5 in the analyzed seasons,with the following rank order(highest to lowest heritabilities):(+)-catechin>(−)-gallocatechin gallate>caffeine=(−)-epicatechin>(−)-epigallocatechin-3-gallate>theanine>(−)-epigallocatechin>(−)-epicatechin-3-gallate>catechin gallate>(+)-*** SNPs detected by amplified-fragment SNP and methylation sequencing divided Population 1 into three groups and seven *** association analysis yielded 307 SNP markers related to theanine,caffeine,and catechins that were common to all three *** of the markers were *** functional annotation of 180 key genes at the SNP loci revealed that FLS,UGT,MYB,and WD40 domain-containing proteins,as well as ATP-binding cassette transporters,may be important for catechin *** and GO analyses indicated that these genes are associated with metabolic pathways and secondary metabolite ***,in Population 2(98 tea plant germplasm resources),30 candidate SNPs were verified,including 17 SNPs that were significantly or extremely significantly associated with specific metabolite *** results will provide a foundation for future research on important flavor-related metabolites and may help accelerate the breeding of new tea varieties.

关键词： analysis breeding Population

PLA2G2D and CHIT1: Potential biomarkers for immune infiltration and prognosis in cervical squamous cell carcinoma

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Frigid Zone Medicine 2024年第2期4卷 110-119页

作者： Liangliang Jiang qiushuang Qiao Jing Wang Department of Gynecological Tumor Harbin Medical University Cancer HospitalHarbin 150001China Department of Pathology Harbin Medical University Cancer HospitalHarbin 150001China

Objective:The aim of this study was to identify biomarkers associated with immunity and prognosis in patients with cervical *** and methods:Data from patients with cervical squamous cell carcinoma(CESC)were retrieved from the UCSC Xena database and subjected to *** sets representing 22 types of immunocytes were acquired,and immunocytes relevant to prognosis were *** gene co-expression network analysis(WGCNA)was utilized to identify gene modules associated with prognosis-related immunocytes and to construct immune-related gene *** expressed genes were then screened,and the association between immune score and biological function of immune-related gene markers was ***,tissue samples from cervical cancer patients in Northeast China were collected to validate the expression of two genes using real-time PCR and ***:This study identified 10 immunocytes significantly correlated with overall survival time in *** gene modules were identified as significantly associated with prognosis-related immunocytes,with gene module 6 showing relevance to all prognosis-related *** module 6 was related to all prognosis-related ***,two genes(including PLA2G2D and CHIT1)were found to be significantly associated with overall survival in cancer *** with CESC were classified into high and low immune score groups based on the median score of gene *** analysis of the immune score and biological function was *** and real-time PCR results revealed high expression of CHIT1 and PLA2G2D in CESC tumor ***:PLA2G2D and CHIT1 show promise as biomarkers for evaluating immune infiltration and prognosis in patients with cervical cancer.

关键词： cervical cancer immune cells overall survival immune score genes

China’s low-emission pathways toward climate-neutral livestock production for animal-derived foods

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The Innovation 2022年第2期3卷 11-13页

作者： Rong Wang Zhaohai Bai Jinfeng Chang qiushuang Li Alexander N.Hristov Pete Smith Yulong Yin Zhiliang Tan Min Wang CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry ProductionInstitute of Subtropical AgricultureThe Chinese Academy of SciencesChangsha 410125China Key Laboratory of Agricultural Water Resources Hebei Key Laboratory of Soil EcologyCenter for Agricultural Resources ResearchInstitute of Genetic and Developmental BiologyChinese Academy of SciencesShijiazhuang 050021China College of Environmental and Resource Sciences Zhejiang UniversityHangzhou 310058China Department of Animal Science The Pennsylvania State UniversityUniversity ParkPA 16802USA Institute of Biological and Environmental Sciences School of Biological SciencesUniversity of AberdeenAberdeen AB243UUUK

Animal-derived food production accounts for 19%of global anthropogenic greenhouse gas(GHG)*** followed in China is ranked as lowcarbon emitting(i.e.,0.21 t CO_(2-)eq per capita in 2018,ranking at 145^(th) of 168 countries)due to the low average animal-derived food consumption rate,and preferential consumption of animal-derived foods with lower GHG emissions(i.e.,pork and eggs versus beef and milk).

关键词： ranked neutral emissions

Roles of Metallic Oxides in the Elimination Reactions of NO

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International Journal of Minerals,Metallurgy and Materials 1997年第4期11卷 15-18页

作者： XU Chunbao WU Shengli SHOU qiushuang CANG Daqiang(Metallurgy Engineering School, USTB, Beijing 100083, China)

The catalytic effect of several metallic oxides(Fe2O3, Cr3O3 and Ni2O3) on elimination of NO has been studied in a flow type reactor, and the roles of metallic oxide in the elimination reactions of NO has also been discussed. The results show that in the system of NO-CO-CO2-N2, the catalytic reaction of NO/CO on the metallic oxide plays a leading role in NO elimination especially for Fe2O3 and Cr2O3 samples. However, Ni2O3 and its pre-reduced samples show extremely remarkable catalytic effect on direct decomposition of NO.

关键词： nitrogen oxides catalytic oxide

Detection and Clinical Significance of Peripheral Blood CTC, CA125, CA153, and CEA Levels in Patients with Ductal Carcinoma in situ

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Proceedings of Anticancer Research 2023年第5期7卷 89-94页

作者： Jirui Sun qiushuang Ma Hong Chen Xing Zhou Jinku Zhang Baoding First Central Hospital Baoding 071000Hebei ProvinceChina

Objective:To explore the detection and clinical significance of peripheral blood CTC,CA125,CA153,and CEA levels in patients with ***:210 patients who received surgical treatment for breast cancer in our hospital from January 2019 to December 2022 were selected as the research *** to the postoperative pathology,100 cases were divided into breast cancer and 110 benign breast tumor *** hundred ten healthy patients undergoing physical examination during the same period were selected as the control ***153,CA125,and CEA levels were observed in the three ***:The levels of CA153,CA125,and CEA in the breast cancer group were significantly higher than those in the benign breast tumor group and the control group(P<0.05);the levels of CA153,CA125,and CEA in the benign breast tumor group were all higher than those in the control group,but the differences were not statistically significant;among the three tumor markers,CA153 has the highest sensitivity at 39.00%,CA125 has the second highest sensitivity at 18.00%,and CEA has the lowest sensitivity at 17.00%;The sensitivity of the two joint tests of CA153+CA125,CA153+CEA,and CA125+CEA for the diagnosis of breast cancer were 50.00%,48.00%,and 26.00%respectively;the sensitivity of the three joint tests is the highest,reaching 53.00%,while the specificity of the joint tests was lower than the individual ***:Detection of peripheral blood CTC,CA125,CA153,and CEA levels has specific reference significance for the treatment and prognosis of DCIS patients.

关键词： Ductal carcinoma in situ CTC CA125 CA153