Genome-wide association analysis identified molecular markers associated with important tea flavor-related metabolites
作者机构：Tea Research InstituteGuangdong Academy of Agricultural SciencesGuangdong Key Laboratory of Tea Plant Resources Innovation&UtilizationGuangzhou 510640China Institute of BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikou 570100China Hainan UniversityHaikou 570228China
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2021年第8卷第1期
页 面：714-730页
核心收录：
学科分类：09[农学] 090203[农学-茶学] 0902[农学-园艺学]
基 金：This work was supported by Key-Area Research and Development Program of Guangdong Province[2020B020220004] the earmarked fund for the National Modern Agro-industry Technology Research System(CARS-19) the National Natural Science Foundation of China(31600550) Science and Technology program of Guangdong Province(2016A030303032 and 2019B030316026) Discipline team building projects of Guangdong Academy of Agricultural Sciences in the 13th Five-Year Period(201619TD) Special funds for scientific innovation strategy-construction of high level Academy of Agriculture Science(R2017PY-QY009) Integrated demonstration of key techniques for the industrial development of featured crops in rocky desertification areas of Yunnan-Guangxi-Guizhou provinces(SMH2019-2021) the Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences(1630052019022)
主 题：analysis breeding Population
摘 要：The characteristic secondary metabolites in tea(theanine,caffeine,and catechins)are important factors contributing to unique tea ***,there has been relatively little research on molecular markers related to these ***,we conducted a genome-wide association analysis of the levels of these tea flavor-related metabolites in three *** theanine,caffeine,and catechin levels in Population 1 comprising 191 tea plant germplasms were examined,which revealed that their heritability exceeded 0.5 in the analyzed seasons,with the following rank order(highest to lowest heritabilities):(+)-catechin(−)-gallocatechin gallatecaffeine=(−)-epicatechin(−)-epigallocatechin-3-gallatetheanine(−)-epigallocatechin(−)-epicatechin-3-gallatecatechin gallate(+)-*** SNPs detected by amplified-fragment SNP and methylation sequencing divided Population 1 into three groups and seven *** association analysis yielded 307 SNP markers related to theanine,caffeine,and catechins that were common to all three *** of the markers were *** functional annotation of 180 key genes at the SNP loci revealed that FLS,UGT,MYB,and WD40 domain-containing proteins,as well as ATP-binding cassette transporters,may be important for catechin *** and GO analyses indicated that these genes are associated with metabolic pathways and secondary metabolite ***,in Population 2(98 tea plant germplasm resources),30 candidate SNPs were verified,including 17 SNPs that were significantly or extremely significantly associated with specific metabolite *** results will provide a foundation for future research on important flavor-related metabolites and may help accelerate the breeding of new tea varieties.
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