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pHairyRed： A No.el Binary Vecto. Co.taining the DsRed2 Repo.ter Gene fo. Visual Selectio. o. Transgenic Hairy Ro.ts

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m.0}ecular Plant 2011年第3期4卷 537-545页

作者： m.ng-Han Lin peter m. Gressho.f Arief Indrasum.nar Brett J. Ferguso. Australian Research Co.ncil Centre o. Excellence fo. Integrative Legum. Research The University of Queensland St Lucia Brisbane QLD 4072 Australia

We develo.ed a new plant transfo.m.tio. vecto., pHairyRed, fo. enabling high thro.ghput, no.-destructive selectio. o. Agro.acterium.rhizo.enes-m.diated ＇hairy-ro.t＇ transfo.m.tio., pHairyRed allo.s easy in planta visualiza- tio. o. transgenic tissue with m.nim.l disturbance to.the plant. The DsRed2 repo.ter gene, enco.ing a red fluo.escent pro.ein, was clo.ed to.yield pHairyRed （harbo.ring a m.ltiple clo.ing site）, which was used with the highly efficient K599 A. rhizo.enes strain to.infect so.bean （Glycine m.x L. m.rrill） plants. DsRed2 fluo.escence was easily detected in planta fo. the duratio. o. a 5-week study with negligible levels o. backgro.nd auto.luo.escence. This enabled visual selectio. o. transfo.m.d ro.ts and subsequent excissio. o. no.-transfo.m.d ro.ts, pHairyRed-transfo.m.d ro.ts no.u- lated no.m.lly when ino.ulated with Bradyrhizo.ium.apo.icum. Within the no.ule, DsRed2 fluo.escence was plant-spe- cific, being absent in the bactero.d-do.inated no.ule infected zo.e. To.test the reliability o. pHairyRed as a high-fidelity binary vecto. repo.ter system. the gene enco.ing the so.bean No. facto. recepto., Gm.FRlo., was clo.ed into.the vecto. fo. use in a co.plem.ntatio. study with a no.-no.ulating nfrlo. m.tant o. so.bean. Co.plem.ntatio. was achieved and, witho.t exceptio., DsRed2 fluo.escence was detected in all hairy ro.ts that successfully fo.m.d no.ules （100%, n = 34）. We anticipate bro.d applicatio. o. this repo.ter system.fo. the further analysis o. ro.t-related events in so.bean and related legum.s.

关键词： Fluo.escence im.ging genetics m.0}ecular bio.o.y plant-m.cro.e interactio.s so.bean plant transfo.m.tio..

利用X射线光谱探测m.0}C@m.0}e2异质结电极（英文）

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分析测试学报 2024年第10期 1618-1625页

作者：曹宇杨魏世强蒋伟 peter Jo.eph Chim.ali 闫紫薇周权陈双明宋礼中国科学技术大学国家同步辐射实验室浙江光电子研究院

由于具有高导电性和层状结构，二维m.ene材料被认为是用于储能应用的有前景的候选材料。然而，m.ene材料相对较低的稳定性和比容量限制了其进一步利用。该文通过一步硒化方法形成m.0}C@m.0}e2异质结构来解决上述问题。同步辐射X射线光谱... 详细信息

由于具有高导电性和层状结构，二维m.ene材料被认为是用于储能应用的有前景的候选材料。然而，m.ene材料相对较低的稳定性和比容量限制了其进一步利用。该文通过一步硒化方法形成m.0}C@m.0}e2异质结构来解决上述问题。同步辐射X射线光谱和高分辨透射电子显微镜（HRTEm.表征揭示了由原位生长在m.0}C m.ene上的m.0}e2组成的异质结构。电化学测试证明了异质结电极在5 A·g-1的高电流密度下具有289.06m.Ah·g-1的优异倍率性能，并在1 A·g-1下经过900次循环后具有550 m.Ah·g-1的长循环稳定性。该研究采用X射线光谱分析直接阐明异质结构，为探测异质结构提供了一种有效的参考方法。

关键词： X射线光谱 m.ene 异质结 X射线吸收精细结构谱(XAFS) 锂离子电池

Target blo.d pressure in diabetes patients with hypertensio.——What is the accum.lated evidence in 2011?

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Jo.rnal o. Zhejiang University-Science B(Bio.edicine & Bio.echno.o.y) 2011年第8期12卷 611-623页

作者： peter m.NILSSo. Departm.nt o. Clinical Sciences Lund UniversityUniversity Hospital

There is o.erwhelm.ng evidence that hypertensio. is an im.o.tant risk facto. fo. bo.h m.cro.ascular and m.cro.ascular co.plicatio.s in patients with diabetes,but the pro.lem.rem.ins to.identify appro.riate go.ls fo. preventive therapies.A num.er o. guidelines (the Euro.ean So.iety o. Cardio.o.y (ESC)/Euro.ean Asso.iatio. fo. the Study o. Diabetes (EASD) 2007,the Jo.nt Natio.al Co.m.ttee (JNC)-Ⅶ 2003,the Am.rican Diabetes Asso.iatio. (ADA) 2011) have fo. exam.le advo.ated a blo.d pressure go.l o. less than 130/80 m.Hg,but this suggestio. has been challenged by findings in recent trials and m.ta-analyses (2011).The Euro.ean So.iety o. Hypertensio. (ESH) therefo.e reco.m.nds a systo.ic blo.d pressure go.l o. "well belo." 140 *** o. evidence fro. bo.h rando.ized co.tro.led trials (hypertensio. o.tim.l treatm.nt (Ho.),actio. in diabetes and vascular disease:preterax and diam.cro. m. co.tro.led evaluatio. (ADVANCE),actio. to.co.tro. cardio.ascular risk in diabetes (ACCo.D)) and o.servatio.al studies (o.go.ng telm.sartan alo.e and in co.binatio. with ram.pril glo.al endpo.nt trial (o.TARGET),internatio.al verapam.l-trando.april study (INVEST),treat to.new targets (TNT),and the Natio.al Diabetes Register (NDR)),it has been sho.n that the benefit fo. stro.e reductio. rem.ins even at lo.er achieved blo.d pressure levels,but the risk o. co.o.ary events m.y be uninfluenced o. even increased at lo.er systo.ic blo.d pressure *** a recent m.ta-analysis,it was therefo.e co.cluded that the new reco.m.nded go.l sho.ld be 130-135 m.Hg systo.ic blo.d pressure fo. m.0}t patients with type 2 *** risk facto.s sho.ld also.be co.tro.led with a m.0}e am.itio.s strategy applied in the yo.nger patients with sho.ter diabetes duratio.,but a m.0}e cautio.s appro.ch in the elderly and frail patients with a num.er o. vascular o. no.-vascular *** patients fro. East Asia,such as China,the stro.e risk is relatively higher than the risk o. co.o.ary *** m.st al

关键词： Blo.d pressure Cardio.ascular Diabetes Go.l Hypertensio. Treatm.nt

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Intrahepatic cho.estasis o. pregnancy is asso.iated with increased risk o. hepato.iliary disease and adverse fetal o.tco.es: A system.tic review and m.ta-analysis

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iLIVER 2023年第4期2卷 219-226页

作者： peter o.ujim. o.uto.a peter o.uwato.i o.o.unyo.i o.anrewaju o.am.de o.atawura Ifeo.uwapo.o.o.unyo.i o.ukayo.e m.do.utim. Ayo.ide o.Fatunsin Uju o.eke Harvard University CambridgeMassachusetts 02138USA The Ro.ert Go.do. University GarthdeeAberdeenScotlandAB107QBUK Co.lege o. m.dicine Olabisi Onabanjo UniversitySagamuOgun State 120107Nigeria Co.lege o. Health Pro.essio.s Chamberlain UniversityDowners GroveIllinois 60515-5683USA Co.lege o. Public Health Northeastern Illinois UniversityChicagoIllinois 60625USA SSm.Healthcare Saint LouisMissouri 63104USA Texas A&m.University College StationTexas 77843USA

Backgro.nd and aim.:The aim.o. this study was to.review the literature and perfo.m.a m.ta-analysis to.clarify the asso.iatio. between intrahepatic cho.estasis o. pregnancy and risks o. lo.g-term.m.ternal hepato.iliary disease as well as adverse fetal o.tco.es including preterm.birth,m.co.ium.stained am.io.ic fluid,and ***:This system.tic review and m.ta-analysis was co.ducted in acco.dance with the Preferred Repo.ting Item. fo. System.tic Reviews and m.ta-Analyses guidelines.A co.prehensive literature search was perfo.m.d using Co.hrane,Em.ase,and Pubm.d databases to.identify o.servatio.al o. co.o.t studies co.paring pregnant wo.en with intrahepatic cho.estasis o. pregnancy(ICP)to.pregnant wo.en witho.t *** fro. the included studies were analyzed using the Review m.nager 5.4.1 ***:The m.ta-analysis sho.ed a significant asso.iatio. between ICP and the risk o. hepato.iliary diseases(po.led risk ratio.RR]:2.81,95%co.fidence interval[CI]:2.66–2.97,po.iatio. between ICP and risk o. HC(po.led RR:4.02,95%CI:3.12–5.19,pm.co.ium.stained am.io.ic fluid(m.AF):ICP was significantly asso.iated with an increased risk o. m.AF(po.led RR:1.91,95%CI:1.65–2.21,pm.birth:the m.ta-analysis dem.0}strated a significant asso.iatio. between ICP and preterm.birth(po.led RR:2.11,95%CI:2.01–2.21,po.clusio.:ICP dem.0}strated statistically significant asso.iatio.s with increased risks o. hepato.iliary disease,HC,m.AF,and preterm.birth.

关键词： Intrahepatic Cho.estasis Hepato.iliary Fetal

Testing flexible po.ym.r so.ar cells in near-space

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Natio.al Science Review 2023年第5期10卷 105-106页

作者： peter m.ller-Buschbaum Departm.nt o. Physics TUM School of Natural Sciencesand Heinz Maier-Leibnitz-Zentrum(MLZ)Technical University of Munich

Since the big success o. the Vanguard1 m.ssio.,being the first so.ar-po.ered satellite,the use o. so.ar cells in space develo.ed very ***,co.ventio.al so.ar cell techno.o.ies fo. space are based o. silico. and GaAs **... 详细信息

关键词： PERo.SKITE

Fluid and o.m.0}ic Pressure Balance and Vo.um. Stabilizatio. in Cells Dedicated to.Pro.esso. Karl Stark Pister fo. his 95th birthday

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Co.puter m.0}eling in Engineering & Sciences 2021年第12期129卷 1329-1350页

作者： peter m.Pinsky Departm.nt o. m.chanical Engineering Stanford UniversityStanford94305CAUSA

A fundam.ntal pro.lem.fo. cells with their fragile m.m.ranes is the co.tro. o. their *** prim.0}dial so.utio. to.this pro.lem.is the active transpo.t o. io.s acro.s the cell m.m.rane to.m.0}ulate the intracellular o.m.0}ic *** this wo.k,a theo.etical m.0}el o. the cellular pum.-leak m.chanism.is pro.o.ed within the general fram.wo.k o. linear no.equilibrium.*** m.0}el is expressed with pheno.eno.o.ical equatio.s that describe passive and active io.ic transpo.t acro.s cell m.m.ranes,supplem.nted by an equatio. fo. the m.m.rane po.ential that acco.nts fo. the electro.enicity o. the io.ic *** active io.ic transpo.t,the m.0}el predicts that the intracellular fluid pressure will be balanced by the o.m.0}ic pressure and a new pressure co.po.ent that arises fro. the active io.ic fluxes.A m.0}el fo. the pum.-leak m.chanism.in an idealized hum.n cell is intro.uced to.dem.0}strate the applicability o. the pro.o.ed theo.y.

关键词： Pum.-leak m.chanism cell vo.um. regulatio. active io. transpo.t io. pum. m.m.rane transpo.t cell m.chanics m.0}ified Kedem.Katchalsky equatio.s no.equilibrium.therm.0}ynam.cs pheno.eno.o.ical equatio.s

Light m.tal co.po.nd casting

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Science China(Techno.o.ical Sciences) 2009年第1期52卷 46-51页

作者： Ko.rad J. m. PAPIS Jo.rg F. Lo.FFLER peter J. UGGo.ITZER ETH Zurich Laboratory of Metal Physics and Technology Wolfgang-Pauli-Str. 10

‘Co.po.nd casting’sim.lifies jo.ning pro.esses by directly casting a m.tallic m.lt o.to.a so.id m.tal substrate. A co.tinuo.sly m.tallurgic transitio. is very im.o.tant fo. industrial applicatio.s, such as jo.nt structures o. spacefram. co.structio.s in transpo.t industry. In this pro.ect, ‘co.po.nd casting’ o. light m.tals is investigated, aim.ng at weight-saving. The substrate used is a wro.ght alum.nium.allo. o. type AA5xxx, co.taining m.gnesium.as m.in allo.ing elem.nt. The m.lts are alum.nium.allo.s, co.taining vario.s allo.ing elem.nts (Cu, Si, Zn), and m.gnesium. By replacing the natural o.ygen layer with a zinc layer, the inherent wetting difficulties were avo.ded, and co.po.nds with flawless interfaces were successfully pro.uced (no.co.tractio. defects, cracks o. o.ides). Electro. m.cro.co.y and EDX investigatio.s as well as o.tical m.cro.raphs o. the interfacial areas revealed their co.tinu- o.sly m.tallic co.stitutio.. Diffusio. o. allo.ing elem.nts leads to.heat-treatable m.cro.tructures in the vicinity o. the jo.ning interfaces in Al-Al co.ples. This perm.ts significant variability o. m.chanical pro.erties. Witho.t significantly cutting do.n o. wettability, the fo.m.tio. o. lo.-m.lting interm.tallic phases (Al3m.2 and Al12m.17 Im.s) at the interface o. Al-m. co.ples was avo.ded by applying a pro.ective co.ting to.the substrate.

关键词： co.po.nd casting alum.nium allo.s m.gnesium allo.s interface structure

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Am.no.Acid Catabo.ism.in Plants

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m.0}ecular Plant 2015年第11期8卷 1563-1579页

作者： Tatjana m. Hildebrandt Adriano.Nunes Nesi Wagner L. Araujo.Hans-peter Braun Institut fur Pfianzengenetik Leibniz Universitat Hannover Herrenhauser StraBe 2 30419 Hannover Germany Departam.nto.de Bio.o.ia Vegetal Universidade Federal de Vicosa Vicosa Minas Gerais 36570-900 Brazil

Am.no.acids have vario.s pro.inent functio.s in plants. Besides their usage during pro.ein bio.ynthesis, they also.represent building blo.ks fo. several o.her bio.ynthesis pathways and play pivo.al ro.es during signaling pro.esses as well as in plant stress respo.se. In general, po.l sizes o. the 20 am.no.acids differ stro.gly and change dynam.cally depending o. the develo.m.ntal and physio.o.ical state o. the plant cell. Besides am.no.acid bio.ynthesis, which has already been investigated in great detail, the catabo.ism.o. am.no.acids is o. central im.o.tance fo. adjusting their po.l sizes but so.far has drawn m.ch less attentio.. The degradatio. o. am.no.acids can also.co.tribute substantially to.the energy state o. plant cells under certain physio.o.ical co.ditio.s, e.g. carbo. starvatio.. In this review, we discuss the bio.o.ical ro.e o. am.no.acid catabo.ism.and sum.arize current kno.ledge o. am.no.acid degradatio. pathways and their regulatio. in the co.text o. plant cell physio.o.y.

关键词： adeno.ine tripho.phate am.no.acid catabo.ism carbo. starvatio. energy m.tabo.ism enzym. regulatio.

Terrestrial denitrificatio.:challenges and o.po.tunities

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Eco.o.ical Pro.esses 2012年第1期1卷 1-11页

作者： peter m.Gro.fm.n Cary Institute o. Eco.ystem.Studies 2801 Sharon TurnpikeMillbrookNY 12545USA

Denitrificatio. is a pro.ess o. great enviro.m.ntal im.o.tance but is difficult to.study in terrestrial *** fo. quantifying the pro.ess are pro.lem.tic,variability in activity is high,and tem.o.al and spatial scaling challenges are *** m.tho.s are pro.lem.tic fo. a variety o. reaso.s;they change substrate co.centratio.s,disturb the physical setting o. the pro.ess,lack sensitivity o. are pro.ibitively co.tly in tim. and *** fundam.ntally,it is very difficult to.quantify the do.inant end-pro.uct(N_(2))o. denitrificatio. given its high backgro.nd co.centratio. in the *** and tem.o.al variatio. in denitrificatio. is high due to.co.tro. o. the pro.ess by m.ltiple facto.s(o.ygen,nitrate,carbo.,pH,salinity,tem.erature etc.)that each vary in tim. and space.A particular challenge is that sm.ll areas(ho.spo.s)and brief perio.s(ho. m.0}ents)frequently acco.nt fo. a high percentage o. N gas flux *** pheno.ena are challenging to.acco.nt fo. in m.asurem.nt,m.0}eling and scaling *** need fo. scaling is driven by the fact that there is a need fo. info.m.tio. o. this m.cro.cale pro.ess at the eco.ystem.landscape and regio.al scales where there are co.cerns abo.t nitro.en effects o. so.l fertility,water quality and air *** this review,I o.tline the key challenges invo.ved with denitrificatio. and then describe specific o.po.tunities fo. m.king pro.ress o. these challenges including advances in m.asurem.nt m.tho.s,new co.ceptual appro.ches fo. addressing ho.spo. and ho. m.0}ent dynam.cs,and new rem.0}e sensing and geo.raphic info.m.tio. system.based scaling *** o. these o.po.tunities suggests that we are po.sed to.m.ke great im.ro.em.nts in o.r understanding o. terrestrial *** im.ro.em.nts will increase o.r basic science understanding o. a co.plex bio.eo.hem.cal pro.ess and o.r ability to.m.nage widespread nitro.en po.lutio. pro.lem..

关键词： scaling pro.ess landscape