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Candida albicans isolated from urine:Phenotypic and molecular identification,virulence factors and antifungal susceptibility

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Asian Pacific journal of Tropical Biomedicine 2017年第7期7卷 624-628页

作者： Laura Wiebusch Adriana Araújo de Almeida-Apolonio Luana Mireli Carbonera Rodrigues Bruna de Paula Bicudo Danielly Beraldo dos Santos Silva Danielle Ferreira Lonchiati Renata pires de Araujo Alexéia Barufatti Grisolia Kelly mari pires de oliveira Faculdade de Ciencias Biologicas e Ambientais Universidade Federal da Grande DouradosDouradosMSBrazil Faculdade de Medicina Universidade Federal de Mato Grosso do SulCampo GrandeMSBrazil Faculdade de Ciencias da Saude Universidade Federal da Grande DouradosDouradosMSBrazil Faculdade de Ciencias Agrarias e Veterinarias Universidade Estadual Paulista“Julio de Mesquita Filho”JaboticabalSPBrazil

objective: To isolate Candida albicans(C. albicans) from the urine of hospitalized patients and assess the virulence factors and antifungal susceptibility profiles of the ***: Yeasts were identified using the chromogenic medium CHRoMagar?, the VITEK~?2 system, hypertonic Sabouraud broth, tobacco agar, polymerase chain reaction,and DNA sequencing. The evaluated virulence factors were proteinase production,phospholipase production, and biofilm production on polystyrene. The broth microdilution technique was used to determine the minimum inhibitory ***: All yeasts isolated from urine were identified as C. albicans using both classical and molecular methods. Although 91.3% of the isolates showed no phospholipase activity, 56.5% showed strong proteinase activity and 91.7% produced biofilm. All microorganisms were sensitive to fluconazole, voriconazole and amphotericin B, but 56.5% of the yeasts showed resistance to ***: C. albicans isolates from urine have a high capacity for virulence and can be associated with infectious processes. Furthermore, the high percentage of isolates resistant to itraconazole is important because this antifungal agent is commonly used to treat fungal infections in the hospital environment.

关键词： Candidiasis Urinary tract Infection Virulence Antifungal susceptibility

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The impact of height-adjustable desks and classroom prompts on classroom sitting time,social,and motivational factors among adolescents

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journal of Sport and Health Science 2023年第1期12卷 97-105页

作者： Bronwyn Sudholz Ana mar.a Contardo Ayala Anna Timperio David W.Dunstan David E.Conroy Gavin Abbott Bernie Holland Lauren Arundell jo Salmon Pomegranate Community Mental Health St John of God Social OutreachBallaratVIC 3350Australia Institute for Physical Activity and Nutrition(IPAN) School of Exercise and Nutrition SciencesDeakin UniversityGeelongVIC 3128Australia Physical Activity Laboratory Baker Heart and Diabetes InstituteMelbourneVIC 3004Australia mary MacKillop Institute for Health Research Australian Catholic UniversityMelbourneVIC 3000Australia Department of Kinesiology Pennsylvania State UniversityUniversity ParkPA 16802USA Department of Preventive Medicine Northwestern UniversityChicagoIL 60611USA The Australian Council for Health and Physical Education and Recreation AbbotsfordVIC 3067Australia

Purpose:This quasi-experimental study examined the impact of height-adjustable desks in combination with prompts to break up prolonged sitting time during class time and identified social and motivational factors associated with breaking up sitting time among ***’perceptions of strategies were also ***:over 17 weeks,1 classroom in a government secondary school in Melbourne,Australia,was equipped with 27 height-adjustable desks and prompts(posters and desk stickers)to break up classroom sitting *** received professional development in the use of the desks and *** group of adolescents(n=55)had 2-5 lessons/week using the height-adjustable desks in an intervention classroom,and a comparison group matched by year level and subject(n=50)was taught in traditional“seated”*** wore an activPAL monitor at baseline(T0),4 weeks(T1),and 17 weeks(T2)and completed a survey at T0 and *** teachers participated in interviews at *** sizes were calculated(d).Results:Linear mixed models found that,compared to the traditional“seated”classrooms,the adolescents in the intervention classroom had significantly lower sitting time(T1:-9.7 min/lesson,d=-0.96;T2:-6.7 min/lesson,d=-0.70)and time spent in sitting bouts>15 min(T2:-11.2 min/lesson,d=-0.62),and had significantly higher standing time(T1:7.3 min/lesson,d=0.84;T2:5.8 min/lesson,d=0.91),number of breaks from sitting(T1:1.3 breaks/lesson,d=0.49;T2:1.8 breaks/lesson,d=0.67),and stepping time(T1:2.5 min/lesson,d=0.66).Intervention classroom adolescents reported greater habit strength(d=0.58),self-efficacy for breaking up sitting time(d=0.75),and indicated that having a teacher/classmate remind them to stand as helpful(d=0.50).Conclusion:This intervention shows promise for targeting sitting behaviors in the classroom and indicates that incorporating social and motivational strategies may further enhance outcomes.

关键词： Adolescent School Sedentary behavior Sitting Standing

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Native and exotic seed dispersal by the stone marten(martes foina):implications for the regeneration of a relict climactic forest in central Portugal

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Integrative Zoology 2019年第3期14卷 280-292页

作者： Lúcia PEREIRA Ana VASQUES Paula MAIA mar.a joão RAMoS PEREIRA Carlos FoNSECA Milena MAToS CESAM-Centre for Environmental and marine Studies-Wildlife Research Unit Department of BiologyUniversity of AveiroAveiroPortugal CESAM-Centre for Environmental and marine Studies-Department of Environment and Planning University of AveiroAveiroPortugal Erasmus University College Life Sciences DepartmentRotterdamthe Netherlands Institute of Biosciences PPGBANDepartment of ZoologyUniversidade Federal do Rio Grande do SulBrazil BioLiving Association FrossosAlbergaria-a-VelhaPortugal

The stone marten(martes foina)is a carnivorous mammal that often consumes fleshy fruits,thus potentially promoting seed *** present study was developed in Bussaco National Forest,central Portugal,and aimed to assess the potential role of the stone marten in dispersing native and exotic plants in different forest landscape *** from stone marten scats and fleshy fruits were collected monthly and were thereafter identified and sowed in a nursery,following a randomized experimental *** emergence was monitored *** linear models were used to test for differences in time and success of emergence between seeds from scats and fruits of 3 native species(Rubus ulmifolius,Arbutus unedo and Celtis australis)and 1 exotic plant species with invasive behavior(Prunus laurocerasus).Fruit consumption by the stone martens significantly increased and accelerated the germination of the native *** but had no effect on the other 2 native species or on the invasive *** suggested that stone martens contribute to gene flow and forest regeneration by dispersing native plant ***,although the germination was not enhanced in the invasive species,the preference of stone martens for these fruits may potentially contribute to the proliferation of *** study represents a contribution to better understanding the fauna and flora interactions,enabling for a more conscious and effective decision-making in forest management.

关键词： germination invasive species martes foina Prunus laurocerasus relict climactic forest

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Efficacy of oral consumption of curcumin/Curcuma longa for symptom improvement in inflammatory bowel disease:A systematic review of animal models and a meta-analysis of randomized clinical trials

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BIoCELL 2022年第9期46卷 2015-2047页

作者： mar.a DE CERQUEIRA ALVES MoNISE oLIVEIRA SANToS NASSIB BEZERRA BUENo oRLANDo RoBERTo PIMENTEL DE ARAújo maríLIA oLIVEIRA FoNSECA GoULART FABIANA ANDRéA MoURA Programa de Pós-Graduacao em Nutricao(PPGNUT) Universidade Federal de Alagoas(UFAL)MaceióAL 57072-970Brazil Instituto de Química e Biotecnologia(IQB) Universidade Federal de Alagoas(UFAL)MaceióAL 57072-970Brazil Programa de Pós-Gradua玢o em Ciências da Saúde(PPGCS) Universidade Federal de Alagoas(UFAL)MaceióAL 57072-970Brazil Programa de Pós-Gradua玢o da Rede Nordeste de Biotecnologia(RENoRBIo) Universidade Federal de Alagoas(UFAL)MaceióAL 57072-970Brazil Programa de Pós-Gradua玢o em Ciências Médicas(PPGCM) Universidade Federal de Alagoas(UFAL)MaceióAL 57072-970Brazil

The roots of the vegetal Curcuma due to its high content of polyphenols,has been used successfully in several clinical *** review assessed the effect of curcumin/Curcuma longa on symptoms and metabolic changes in inflammatory bowel disease(IBD).A systematic review of animal models and randomized clinical trials(RCTs)was conducted by searching the following electronic databases:PubMed,CENTRAL,LILACS,Science Direct,and *** 997 found records,62 were *** than 90%of the animal studies reported an improvement in macroscopic,histologic and/or functional activity;80%identified decreased oxidative and/or inflammatory biomarkers in animals treated with *** the RCTs,intention-to-treat analysis showed that oral curcumin was effective in inducing clinical remission(n=281,RR:3.15 CI 95%[1.22–8.10]p=0.0017;i2=72.2%,p=0.006)and clinical response(n=259,RR:1.60 CI 95%[1.09–2.35]p=0.0017;i2=59.7%,p=0.042)but not endoscopic remission(n=161,RR:2.91 CI 95%[0.58–14.58]p=0.195;i2=72.7%,p=0.026).These results confirm that oral supplementation with curcumin/Curcuma longa has beneficial actions in animal colitis and,when associated with drug therapy,is effective in the treatment of patients with IBD.

关键词： Ulcerative colitis Crohn’s disease oxidative stress Curcuma Turmeric

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Availability, Prices and Affordability of Glaucoma Medicines among Private Pharmacies of Nampula City in Mozambique

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open journal of Epidemiology 2022年第2期12卷 158-169页

作者： joel Bambamba Neivaldo Murrube Paulo pires Ângela Bambamba Christpina Anale Avelino Mazuze joão Mazalo Momade Fumo Ramos Manuel Hermenegildo Tomo Department of optometry Faculty of Health Sciences Lúrio University Nampula Mozambique Department of Pharmacy Faculty of Health Sciences Lúrio University Nampula Mozambique Department of Family Medicine Faculty of Health Sciences Lúrio University Nampula Mozambique

Introduction: Glaucoma is a group of chronically progressive disorders of the optic nerve and a worldwide leading cause of irreversible vision loss. Eye chronic diseases including glaucoma are major public health problems around the world, rapidly increasing with a growing and aging population. The treatment of chronic diseases lasts a lifetime. The purpose of this study is to assess the availability, prices and affordability of the medicines for glaucoma management in private pharmacies of Nampula City in Mozambique. Material and Methods: The standardized methodology designed by the World Health organization and Health Action International was employed to conduct the study about the availability, price and affordability of glaucoma medicines in Nampula City from october to November 2021. Data were collected in 39 private pharmacies using a survey with fifteen glaucoma Medicines. Results: The Average of medicines availability was 46.6% (0.0% - 71.8%) with a mean of 8.86. The availability level demonstrated that 14 (93.3%) of all surveyed glaucoma medicines were very low and 1 (6.67%) was fairly high. Timolol was the most available medicine, found in 28 (71.8%) while apraclonidine, carteolol, levobunolol, carbachol, brinzolamide, bimatoprost, travoprost and unoprostone were not available. The medicine with the lowest price was latanoprost (2.84 USD) and the higher was acetazolamide (23.58 USD). None of the surveyed medicines were considered affordable. Conclusion: The majority of surveyed glaucoma medicines were not available and they were totally unaffordable against the defined thresholds. Policy strategy and technical options should be driven and implemented by the government to ensure the availability and affordability of glaucoma medicines at various levels of the Mozambican healthcare system.

关键词： Availability Prices Affordability Medicines Glaucoma

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MYC and gastric adenocarcinoma carcinogenesis

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World journal of Gastroenterology 2008年第39期14卷 5962-5968页

作者： Danielle Queiroz Calcagno mar.ana Ferreira Leal Paulo Pimentel Assumpo marília de Arruda Cardoso Smith Rommel Rodríguez Burbano Human Cytogenetics Laboratory Biology Science InstituteFederal University of ParáBelém PA 66075-900Brazil Genetics Division Department of Morphology and GeneticsFederal University of So PauloSo Paulo SP 04023-900Brazil joo de Barros Barreto University Hospital Federal University of ParáBelém PA 66073-000Brazil

MYC is an oncogene involved in cell cycle regulation, cell growth arrest, cell adhesion, metabolism, ribosome biogenesis, protein synthesis, and mitochondrial function. It has been described as a key element of several carcinogenesis processes in humans. Many studies have shown an association between MYC deregulation and gastric cancer. MYC deregulation is also seen in gastric preneoplastic lesions and thus it may have a role in early gastric carcinogenesis. Several studies have suggested that amplification is the main mechanism of MYC deregulation in gastric cancer. In the present review, we focus on the deregulation of the MYC oncogene in gastric adenocarcinoma carcinogenesis, including its association with Helicobacterpylori （Hpylon] and clinical applications.

关键词： MYC Gastric adenocarcinoma Gastric preneoplastic lesions Gastric carcinogenesis Helicobacter pylori

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Suppression of the External MitochondrialNADPH Dehydrogenase, NDB1, in Arabidopsisthaliana Affects Central Metabolism andVegetative Growth

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Molecular Plant 2014年第2期7卷 356-368页

作者： Sabá V. Wallström Igor Florez-Sarasa Wagner L. Araújo mari Aidemark mar.a Fernández-Fernández Alisdair R. Fernie Miquel Ribas-Carbó Allan G. Rasmusson Department of Biology Lund University Biology building Sölvegatan 35 SE-223 62 Lund Sweden Grup de Recerca en Biologia de les Plantes en Condicions Mediterrànies Universitat de les Illes Balears Ctra Valldemossa Km. 75 07122 Palma de Mallorca Spain Present address: Max-Planck-Institut für Molekulare Pflanzenphysiologie Am Mühlenberg 1 14476 Potsdam-Golm Germany Max-Planck-Institut für Molekulare Pflanzenphysiologie Am Mühlenberg 1 14476 Potsdam-Golm Germany Present address: Departamento de Biologia Vegetal Universidade Federal de Viçosa 36570-000 Viçosa Minas Gerais Brasil

Ca2＋-dependent oxidation of cytosolic NADPH is mediated by NDB1, which is an external type II NADPHdehydrogenase in the plant mitochondrial electron transport chain. Using RNA interference, the NDB1 transcript wassuppressed by 80% in Arabidopsis thaliana plants, and external Ca2＋-dependent NADPH dehydrogenase activity becameundetectable in isolated mitochondria. This was linked to a decreased level of NADP＋ in rosettes of the transgenic ***-grown transgenic seedlings displayed decreased growth specifically on glucose, and respiratory metabolism of 14C-glucose was increased. on soil, NDBl-suppressing plants had a decreased vegetative biomass, but leaf maximumquantum efficiency of photosystem Ⅱ and Co2 assimilation rates, as well as total respiration, were similar to the wild-type. The in vivo alternative oxidase activity and capacity were also similar in all genotypes. Metabolic profiling revealeddecreased levels of sugars, citric acid cycle intermediates, and amino acids in the transgenic lines. The NDBl-suppressioninduced transcriptomic changes associated with protein synthesis and glucosinolate and jasmonate metabolism. Thetranscriptomic changes also overlapped with changes observed in a mutant lacking ABAINSENSITIVE4 and in A. thalianaoverexpressing stress tolerance genes from rice. The results thus indicate that A. thaliana NDB1 modulates NADP（H）reduction levels, which in turn affect central metabolism and growth, and interact with defense signaling.

关键词： citric acid cycle metabolic profiling mitochondrial respiration NADPH RNA interference type II NAD(P)Hdehydrogenase vegetative growth

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Effects of Dexamethasone, Clonidine, Tramadol and Nalbuphine on Fentanyl-Induced Hyperalgesia in Rats

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journal of Biosciences and Medicines 2021年第12期9卷 87-97页

作者： Camila dos Santos Leite Naira Correia Cusma Pelógia Eliane Stevanato marília Hidalgo Uchôas mar.a Helena Rovani pires oscar César pires Laboratory of Immunopharmacology and Molecular Biology S&atilde o Francisco University Medical School Bragan&ccedil a Paulista Brazil Laboratory of Cell and Molecular Tumor Biology and Bioactive Compounds S&atilde o Francisco University Medical School Bragan&ccedil a Paulista Brazil Laboratory of Pharmacology and Physiology Medicine Department Taubaté University Taubaté Brazil Vera Cruz Hospital Campinas Brazil

opioids are drugs used to alleviate pain. However, studies have demonstrated that these drugs can cause an increase in pain sensitivity, which is called opioid-induced hyperalgesia. The objective of this study was to describe the effects of dexamethasone, clonidine, tramadol and nalbuphine on fentanyl-induced hyperalgesia in rats. After obtaining approval from the Committee for the Ethical Use of Animals (CEUA), 36 male Wistar rats were divided into 6 groups: Group 1 (GCSSL) wherein the rats received 1 ml 0.9% saline solution in two injections;Group 2 (GFTSL), received fentanyl at a dose of 100 ug·kg-1 followed by 1 ml 0.9% saline solution via intraperitoneal;the remaining groups (3, 4, 5, 6) received fentanyl at a dose of 100 ug·kg-1 following doses via intraperitoneal: Group 3 (GFTDX), dexamethasone at a dose of 1.0 mg·kg-1;Group 4 (GFTCL), clonidine at a dose of 20 mg·kg-1;Group 5 (GFTTR), tramadol at a dose of 50 mg·kg-1, and Group 6 (GFTNB), nalbuphine at a dose of 5 mg·kg-1. Under general anestesia using isoflurane, the animals were submitted to a surgical incision. Hyperalgesia was evaluated by applying Von Frey filaments at 2 hours after the incision and on the 1st, 3rd and 5th days afterward. At 2 hours after the surgical procedure, there was lower intensity of pain in the fentanyl group (GFTSL) compared to the other groups, and on the fifth day there were no significant differences for pain intensity between groups. The results suggest the presence of fentanyl-induced hyperalgesia and efficacy in its reduction by dexamethasone, clonidine, tramadol and nalbuphine.

关键词： Hyperalgesia Fentanyl Dexamethasone Clonidine Tramadol Nalbuphine Rats

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No evidence of HPV DNA in esophageal squamous cell carcinoma in a population of Southern Brazil

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World journal of Gastroenterology 2013年第39期19卷 6598-6603页

作者： Luís Carlos Moreira Antunes joo Carlos Prolla Antonio de Barros Lopes mar.a pires da Rocha Renato Borges Fagundes Programa de Pós-Graduao: Ciências em Gastroenterologia e Hepatologia - Universidade Federal do Rio Grande do Sul Porto AlegreRio Grande do Sul 90035-003Brasil Hospital Universitário de Santa mar.a - Universidade Federal de Santa mar.a Santa MariaRio Grande do Sul 97119-900Brasil Departamento de Clínica Médica - Universidade Federal de Santa mar.a Santa MariaRio Grande do Sul 97119-900Brasil

AIM:To investigate the association between human papillomavirus(HPV)and esophageal squamous cell carcinoma(ESCC)in southern ***:We studied 189 esophageal samples from125 patients from three different groups:(1)102 biopsies from 51 patients with ESCC,with one sample from the tumor and another from normal esophageal mucosa distant from the tumor;(2)50 esophageal biopsies from 37 patients with a previous diagnosis of head and neck squamous cell carcinoma(HNSCC);and(3)37 biopsies from esophageal mucosa with normal appearance from 37 dyspeptic patients,not exposed to smoking or alcohol ***-polymerase chain reaction(PCR)with the MY09/11 and GP5/6 L1primers was used to detect HPV L1 in samples fixed in formalin and stored in paraffin *** PCR reactions were performed with a positive control(cervicovaginal samples),with a negative control(Human Genomic DNA)and with a blank reaction containing all reagents except *** took extreme care to prevent DNA contamination in sample collection,processing,and ***:The histological biopsies confirmed the diagnosis of ESCC in 52 samples(51 from ESCC group and 1 from the HNSCC group)and classified as well differentiated(12/52,23.1%),moderately differentiated(27/52,51.9%)or poorly differentiated(7/52,13.5%).one hundred twenty-eight esophageal biopsies were considered normal(51 from the ESCC group,42 from the HNSCC group and 35 from dyspeptic patients).Nine had esophagitis(7 from the HNSCC and 2 from dyspeptic patients).of a total of 189 samples,only 6 samples had insufficient material for PCR analysis:1 from mucosa distant from the tumor in a patient with ESCC,3from patients with HNSCC and 2 from patients without *** 183 samples(96.8%)GAPDH,G3PDH and/orβ-globin were amplified,thus indicating the adequacy of the DNA in those *** DNA was negative in all the 183 samples tested:52 with ESCC,9 with esophagitis and 122 with normal esophageal ***:There was no evidence of HP

关键词： Esophageal cancer Esophageal squamous cell carcinoma Human papillomavirus Head and neck cancer Polymerase chain reactions Nested-polymerase chain reaction

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Curcumin in inflammatory bowel diseases:Cellular targets and molecular mechanisms

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BIoCELL 2023年第11期47卷 2547-2566页

作者： AMYLLY SANUELLY DA PAZ marTINS mar.a DE CERQUEIRA ALVES oRLANDo RoBERTo PIMENTEL DE ARAÚjo FABIANA oLIVEIRA DoS SANToS CAMATARI marÍLIA oLIVEIRA FoNSECA GoULART FABIANA ANDRÉA MoURA Rede Nordeste de Biotecnologia(RENoRBIo) Instituto de química e BiotecnologiaUniversidade Federal de Alagoas(UFAL)Maceió57072-900Brasil Programa de Pós-graduação em Nutrição(PPGNUT) Faculdade de Nutrição(FANUT)Universidade Federal de Alagoas(UFAL)Maceió57072-900Brasil Programa de Pós-Graduação em Química e Biotecnologia(PPGQB) Instituto de química e BiotecnologiaUniversidade Federal de Alagoas(UFAL)Maceió57072-900Brasil Colegiado de Nutrição Universidade de Pernambuco(UPE)Campus PetrolinaPernambuco56328-900Brasil Programa de Pós-Graduação em Ciências da Saúde(PPGCS) Instituto de Ciências da SaúdeUniversidade Federal de Alagoas(UFAL)Maceió57072-900Brasil Programa de Pós-graduação em Ciências Médicas(PPGCM) Faculdade de MedicinaUniversidade Federal de Alagoas(UFAL)Maceió57072-900Brasil

Curcumin,a natural product,has exhibited promising effects in both animal models and clinical trials,interacting with a multitude of factors linked to Inflammatory Bowel Disease(IBD).These factors encompass cytokines,oxidative stress-associated enzymes,and modulation of the intestinal ***,curcumin has demonstrated therapeutic potential in animal models of colitis,wherein it exerts a negative regulatory influence on pivotal signaling pathways such as PI3/Akt,JAK/STAT,andβ-***,it inhibits the expression of proinflammatory enzymes and co-stimulatory molecules(including RANKL,ICAM-1,CD205,CD256,TLR4,among others),while curbing immune cell chemotaxis,thereby attenuating the characteristic neutrophil infiltration observed in *** facet of curcumin’s action involves its modulation of the intestinal ***,the microbiota itself contributes to beneficial biotrans formations of curcumin,thereby enhancing its effectiveness in IBD *** a clinical front,curcumin has demonstrated the ability to induce clinical and/or endoscopic remission without any reported toxic ***,curcumin warrants consideration as an adjunctive therapy in IBD *** clinical investigations should concentrate on meticulously evaluating curcumin’s impact on these precise therapeutic targets.

关键词： Curcuminoids Polyphenols CDAI scores Ulcerative colitis Antioxidant

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