Effects of Dexamethasone, Clonidine, Tramadol and Nalbuphine on Fentanyl-Induced Hyperalgesia in Rats

作     者：Camila dos Santos Leite Naira Correia Cusma Pelógia Eliane Stevanato Marília Hidalgo Uchôas Marta Helena Rovani Pires Oscar César Pires Camila dos Santos Leite;Naira Correia Cusma Pelógia;Eliane Stevanato;Marília Hidalgo Uchôas;Marta Helena Rovani Pires;Oscar César Pires

作者机构：Laboratory of Immunopharmacology and Molecular Biology Sã o Francisco University Medical School Braganç a Paulista Brazil Laboratory of Cell and Molecular Tumor Biology and Bioactive Compounds Sã o Francisco University Medical School Braganç a Paulista Brazil Laboratory of Pharmacology and Physiology Medicine Department Taubaté University Taubaté Brazil Vera Cruz Hospital Campinas Brazil 

出 版 物：《Journal of Biosciences and Medicines》 (生物科学与医学（英文）)

年 卷 期：2021年第9卷第12期

页      面：87-97页

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

主　　题：Hyperalgesia Fentanyl Dexamethasone Clonidine Tramadol Nalbuphine Rats 

摘      要：Opioids are drugs used to alleviate pain. However, studies have demonstrated that these drugs can cause an increase in pain sensitivity, which is called opioid-induced hyperalgesia. The objective of this study was to describe the effects of dexamethasone, clonidine, tramadol and nalbuphine on fentanyl-induced hyperalgesia in rats. After obtaining approval from the Committee for the Ethical Use of Animals (CEUA), 36 male Wistar rats were divided into 6 groups: Group 1 (GCSSL) wherein the rats received 1 ml 0.9% saline solution in two injections;Group 2 (GFTSL), received fentanyl at a dose of 100 ug·kg-1 followed by 1 ml 0.9% saline solution via intraperitoneal;the remaining groups (3, 4, 5, 6) received fentanyl at a dose of 100 ug·kg-1 following doses via intraperitoneal: Group 3 (GFTDX), dexamethasone at a dose of 1.0 mg·kg-1;Group 4 (GFTCL), clonidine at a dose of 20 mg·kg-1;Group 5 (GFTTR), tramadol at a dose of 50 mg·kg-1, and Group 6 (GFTNB), nalbuphine at a dose of 5 mg·kg-1. Under general anestesia using isoflurane, the animals were submitted to a surgical incision. Hyperalgesia was evaluated by applying Von Frey filaments at 2 hours after the incision and on the 1st, 3rd and 5th days afterward. At 2 hours after the surgical procedure, there was lower intensity of pain in the fentanyl group (GFTSL) compared to the other groups, and on the fifth day there were no significant differences for pain intensity between groups. The results suggest the presence of fentanyl-induced hyperalgesia and efficacy in its reduction by dexamethasone, clonidine, tramadol and nalbuphine.