Candida albicans isolated from urine:Phenotypic and molecular identification,virulence factors and antifungal susceptibility

作     者：Laura Wiebusch Adriana Araújo de Almeida-Apolonio Luana Mireli Carbonera Rodrigues Bruna de Paula Bicudo Danielly Beraldo dos Santos Silva Danielle Ferreira Lonchiati Renata Pires de Araujo Alexéia Barufatti Grisolia Kelly Mari Pires de Oliveira 

作者机构：Faculdade de Ciencias Biologicas e AmbientaisUniversidade Federal da Grande DouradosDouradosMSBrazil Faculdade de MedicinaUniversidade Federal de Mato Grosso do SulCampo GrandeMSBrazil Faculdade de Ciencias da SaudeUniversidade Federal da Grande DouradosDouradosMSBrazil Faculdade de Ciencias Agrarias e VeterinariasUniversidade Estadual Paulista“Julio de Mesquita Filho”JaboticabalSPBrazil 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2017年第7卷第7期

页      面：624-628页

核心收录：

学科分类：1010[医学-医学技术（可授医学、理学学位）] 10[医学] 

基　　金：supported by Fundacao de Apoio ao Desenvolvimento do Ensino Ciência e Tecnologia de Mato Grosso do Sul(Grant number:0068/12 and SIAFEM:020666) 

主　　题：Candidiasis Urinary tract Infection Virulence Antifungal susceptibility 

摘      要：Objective: To isolate Candida albicans(C. albicans) from the urine of hospitalized patients and assess the virulence factors and antifungal susceptibility profiles of the ***: Yeasts were identified using the chromogenic medium CHROMagar?, the VITEK~?2 system, hypertonic Sabouraud broth, tobacco agar, polymerase chain reaction,and DNA sequencing. The evaluated virulence factors were proteinase production,phospholipase production, and biofilm production on polystyrene. The broth microdilution technique was used to determine the minimum inhibitory ***: All yeasts isolated from urine were identified as C. albicans using both classical and molecular methods. Although 91.3% of the isolates showed no phospholipase activity, 56.5% showed strong proteinase activity and 91.7% produced biofilm. All microorganisms were sensitive to fluconazole, voriconazole and amphotericin B, but 56.5% of the yeasts showed resistance to ***: C. albicans isolates from urine have a high capacity for virulence and can be associated with infectious processes. Furthermore, the high percentage of isolates resistant to itraconazole is important because this antifungal agent is commonly used to treat fungal infections in the hospital environment.