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Aagab is required for zebrafish larval development by regulating neural activity

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Journal of Genetics and Genomics 2024年第6期51卷 630-641页

作者： Shihui Ding Tursunjan Aziz anming meng Shunji Jia State Key laboratory of Membrane biology Tsinghua-Peking Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China Guangzhou laboratory GuangzhouGuangdong 510320China State Key laboratory of Molecular developmental biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China

Clathrin-mediated endocytosis has been implicated in various physiological processes,including nutrient uptake,signal transduction,synaptic vesicle recycling,maintenance of cell polarity,and antigen *** prior knowledge of its importance as a key regulator in promoting clathrin-mediated endocytosis,the physiological function of α-and γ-adaptin binding protein(aagab)remains *** this study,we investigate the biological function of aagab during zebrafish *** establish a loss-of-function mutant of aagab in zebrafish,revealing impaired swimming and early larval *** the high expression level of aagab in the brain,we probe into its physiological role in the nervous *** mutants display subdued calcium responses and local field potential in the optic tectal neurons,aligning with reduced neurotransmitter release(e.g.,norepinephrine)in the tectal neuropil of aagab *** aagab mRNA or nervous stimulant treatment in mutants restores neurotransmitter release,calcium responses,swimming ability,and ***,our observations show delayed release of FM 1-43 in AAGAB knockdown differentiated neuroblastoma cells,pointing towards a probable link to defective clathrin-mediated synaptic vesicle *** conclusion,our study underscores the significance of Aagab in neurobiology and suggests its potential impacts on neurological disorders.

关键词： Aagab Endocytosis Neural activity Synaptic transmitter release Zebrafish

Crystallization and preliminary X-ray studies of porcine kidney aminoacylase Ⅰ

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Chinese Science Bulletin 1997年第20期42卷 1750-1752页

作者： ZHAOHaiyan, mengWuyi, lin Zhengjiong and ZHOU Haimeng1. National laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of sciences, beijing 100101, china 2. department of biological Science and biotechnology, tsinghua university, beijing 100084, china National laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing China department of biological Science and biotechnology Tsinghua University Beijing China

AMINOACYLASE Ⅰ（ACY-1）（E. C. 3. 5. 1. 14 ） participated in amino acid metabolism existingin mammalian kidney and microorganisms, and catalyzes reversible hydrolysis of acylaminoacids. ACY-1 consists of 772 amino acids, and the molecular weight is 85 500 daltons. It is adimeric metalloprotein having two Zn2+ in the molecule. The nucleotide and the amino acid se-quence of porcine and human ACY-1 have been determined. The nucleotide sequence and

关键词： aminoacylase I crystallization preliminary crystallography.

Evaluation of non-covalent interaction between Seryl-Histidine dipeptide and cyclophilin A using NMR and molecular modeling

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Science china Chemistry 2010年第9期53卷 1987-1993页

作者： LIU Yan1,SHI YanHong2,LIU XiaoXia1,lin MingKun1,lin DongHai1,2 & ZHAO YuFen1,3 1Key laboratory for Chemical biology of Fujian Province,department of Chemical biology,department of Chemistry,College of Chemistry and Chemical Engineering,Xiamen university,Xiamen 361005,china 2Shanghai Institute of Materia Medica,Shanghai Institutes of biological sciences,Chinese Academy of sciences,Shanghai 201203,china 3Key laboratory for Bioorganic Phosphorus Chemistry and Chemical biology,Ministry of Education,department of Chemistry,School of Life sciences and Engineering,tsinghua university,beijing 100084,china Key laboratory for Chemical biology of Fujian Province Department of Chemical Biology Department of Chemistry College of Chemistry and Chemical Engineering Xiamen University Xiamen China Shanghai Institute of Materia Medica Shanghai Institutes of Biological Sciences Chinese Academy of Sciences Shanghai China Key laboratory for Bioorganic Phosphorus Chemistry and Chemical biology Ministry of Education Department of Chemistry School of Life Sciences and Engineering Tsinghua University Beijing China

Seryl-Histidine dipeptide(Ser-His) has been previously reported to be capable of cleaving DNAs and carboxyl esters,as well as *** protein cleavage mechanism has not been addressed *** an initial step of protein cleavage activity,the non-covalent binding affinity of Ser-His for proteins is a crucial *** this work,we took cyclophilin A(CyPA) as a substrate protein,and evaluated the non-covalent interaction between CyPA and Ser-His using a combination of NMR spectroscopy and molecular modeling *** independent Ser-His binding sites on CyPA were detected using 15N-1H heteronuclear single-quantum coherence(HSQC) *** binding site binds one Ser-His *** constants,Kd1 and Kd2,were estimated to be 2.07 and 6.66 mmol/L,respectively,indicative of the weak non-covalent interaction between Ser-His and *** on molecular modeling results,we suggest that both the α-amino and the side chain hydroxyl group of Ser-His are crucial for the non-covalent interaction between Ser-His and *** work sheds light on the molecular mechanism of Ser-His and its analogues cleaving proteins.

关键词： Ser-His cyclophilin A HSQC non-covalent interaction binding sites

Kinetochore proteins in zebrafish development and cancer

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Kinetochore proteins in zebrafish development and cancer

第三届细胞结构与功能的信号基础研讨会

作者： anming meng Protein Science laboratory of Ministry of Education,department of biological sciences and biotechnology,tsinghua university,beijing 100084,china State Key laboratory of Biomembrane and Membrane Engineering,Institute of Zoology, Chinese Academy of sciences,beijing 100101,china

Kinetochore proteins associate with centromeric DNA and spindle microtubules and play essential roles in chromosome segregation during *** is a constitutive component of the inner kinetochores,but its importance in development is *** this study,we uncovered a zebrafish mutant,stagnant and curly(stac), which carries the Tol2-transposon element inserted at the cenph *** embryos exhibit discernible cell death as early as 20 hpf,extensive apoptosis in the head and spinal cord and upward curly tail at later stages,and deform around 5 dpf. The stac mutant phenotype can be rescued by cenph mRNA overexpression,and mimicked by cenph knockdown with antisense morpholinos,suggesting the responsibility of cenph for stac *** demonstrate that the intrinsic apoptosis pathway is hyperactivated in stac mutants and that p53 knockdown temporarily blocks excess apoptosis in stac *** cells in stac mutants show chromosome missegregation and are usually arrested in G2/M ***, compared to wildtype siblings,heterozygous stac fish develop invasive tumors at a dramatically reduced level,suggesting a reduced cancer *** together,our findings uncover an essential role of cenph in mitosis and embryonic development and its association with tumor development.

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Amip inhibits mesoderm induction activity of Nodal in zebrafish

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Amip inhibits mesoderm induction activity of Nodal in zebraf...

中国细胞生物学学会第八届会员代表大会暨学术大会

作者： Lixia Zhang, Hu Zhou, Ye-Guang Chen and anming meng department of biological sciences and biotechnology, The State Key laboratory of Biomembrane and Membrane biotechnology, tsinghua university, beijing 100084, china

Nodal is a TGFβ-related factor and has been identified as a key endogenous inducerfor both mesoderm and endoderm during vertebrate embryogenesis. In zebrafish,concurrent deficiency of Sqt and Cyc, two Nodal-related factors, lead to absence ofmost mesodermal and endodermal tissues in embryos. Here we report a zebrafishgene amip that encodes a novel component of the Nodal signaling pathway inzebrafish. amip mainly expresses in the territories where Sqt and Cyc have inductiveactivities during midblastula and gastrulation period. The expression of amip isnegatively autoregulated but activated by Nodal signaling. Knocking-down of Amip

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Crystallization and Preliminary Crystallographic Analysis of Histone Demethylase PHF8

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生物物理学报 2009年第S1期25卷 320-321页

作者： lin Yu, Yang Wang, Zhongzhou Chen State Key laboratory of Agrobiotechnology, College of biological sciences, china Agricultural university, beijing 100193, china

The reversible process of histone methylation and demethylation plays important roles of epigenetic regulation1. For example, Truncating mutations in human PHF8 gene

关键词： Histone Demethylase PHF8 Structure Protein Crystallography

The roles of axinl and axin2 in left-right asymmetry in zebrafish embryogenesis

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The roles of axinl and axin2 in left-right asymmetry in zebr...

中国遗传学会“发育、遗传和疾病”研讨会

作者： Zhang M lin SC meng A State Key laboratory of Biomembrane and Membrane biotechnology,Protein sciences laboratory of the Ministry of Education,department of biological sciences and biotechnology,tsinghua university,beijing 100084,china.

As a muitidomain protein,Axin has been identified to regulate several signaling pathways, including the Wnt pathway and the JNK pathway,by serving as a scaffold protein,controlling diverse cellular functions in proliferation,fate determination and suppression of *** many functions of Axin in embryogenesis have been studied,its roles in LR asymmetry are still not well ***,our observation of the intrinsic left-right defects in mbl mutant embryos drove us to identify the roles of Axins in left-right asymmetry in *** morpholino knock down experiments, we found down-regulating the expression of axinl and axin2 can both disrupt the expression pattern of the LR genes lft1,lft2 and *** the Wnt signaling and the JNK signaling by knocking down the endogenousβ-catenin and jnkl with specific morpholinos exhibites the similar results to that of axins. Furthermore,we found that the morpholino mediated knock down of β-catenin andjnkl can affect not only the expression of ntl and dyn but also the size of ***,our findings indicate that Axin can also disrupt the normal LR patterning in early steps in zebrafish through the JNK signaling pathway besides the Wnt signaling.

关键词： The roles of axinl and axin2 in left-right asymmetry in zebrafish embryogenesis

smad2 and smad3 are required for mesendoderm induction and neural patterning in zebrafish

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smad2 and smad3 are required for mesendoderm induction and n...

2010·第一届斑马鱼学术研讨会

作者： Shunji Jia~1,Di Wu~1,Cencan Xing~1,anming meng~(1,2,*) 1.department of biological sciences and biotechnology,tsinghua university,beijing 100084, china 2.State Key laboratory of Biomembrane and Membrane Engineering,Institute of Zoology, Chinese Academy of sciences,beijing 100101,china

The *** Nodal,Activin,and Vg1 play important roles in mesendoderm induction and patterning during vertebrate *** ligands are believed to transduce the signal through the receptor-activated transcription factors Smad2 ...

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Characterization and expression pattern of pouII1,a novel class Ⅱ POU gene in zebrafish

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Chinese Science Bulletin 2001年第18期46卷 1523-1527,1586页

作者： LI Ming CAO Ying ZHAO Zhixing lin shuo meng anming Institute of Cellular and developmental biology Department of Biological Science and Biotechnologyand the Protein Science Laboratory of MOETsinghua UniversityBeijing 100084China Institute for Molecular Medicine and Genetics Medical College of GeorgiaGA30912USA

POU domain transcription factors that share a conserved DNA-binding domain, POU domain, are important regulators for the development of embryos in various animal species. A novel zebrafish POU domain gene, poulll has been cloned. The poullll cDNA is 2080 kb in length and encodes a putative polypeptide of 596 ammo acids. It is placed into class II POU family since it shares a high degree of homology with the known members of this family. Northern hybridization identifies a major transcript of approximately 2.1 kb that was present in embryos at the single-cell stage throughout 24 h postfertilization. The whole mount in situ hybridization shows that poulll transcripts are present in the single-cell embryos, strongly suggesting that these transcripts are of maternal origin. During early development of the embryos, poulll mRNA was ubiquitously distributed in all cells and tissues. The transcripts are gradually limited to brains and become completely undetectable by day 3. To our knowledge, poulll is the

关键词： POU zebrafish maternal gene embryogenesis embryos expression pattern.