Tissue-specific expression of GFP reporter gene in germline driven by GATA-2 promoter and enhancers in zebrafish

作     者：MENG Anming & LIN Shuo1. Laboratory of Developmental Biology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China 2. Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA 

作者机构：Laboratory of Developmental Biology Department of Biological Sciences and Biotechnology Tsinghua University Beijing China Institute of Molecular Medicine and Genetics Medical College of Georgia Augusta USA 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2000年第45卷第1期

页      面：31-34,97页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：清华大学校科研和教改项目 

主　　题：zebrafish transgenic GATA-2 GFP germline. 

摘      要：GATA-2, a transcription factor, is expressed in several types of blood cells and in the central nervous system (CNS), and regulates the differentiation of these cells. We have obtained five zebrafish transgenic germlines that carry and express the green fluorescent protein (GFP) gene ligated to various 5’ flanking sepuences of zebrafish GATA-2 gene. The spatial pattern of GFP expression varies, mainly depending on which regulatory sequence is used, among the germlines. In some of the germlines, the expression of GFP is restricted to the CNS and the enveloping layer (EVL) cells, while in some other lines GFP is observed only in the CNS. it is noted that the intensity of GFP in the transgenic fish remain unchanged after a six-generation passage of the transgenes. The transgenic fish could find its uses in the future in generating tissue-specific, even cellspecific mutant fish and in functional study of related genes through transgenesis.