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e.0}ssions from.the.com.ustion of e.calypt and pine.chips in a fluidize. be. re.ctor

Journal of e.vironm.ntal Scie.ce. 2016年第4期28卷 246-258页

作者： e.D.Vice.te.L.A.C.Tare.ho e.R.Te.xe.ra m.duarte T.Nune. C.Colom.i V.Giane.le.G.O.da Rocha A.Sanche. de.la Cam.a C.A.Alve. Ce.tre.for e.vironm.ntal and m.rine.Studie. Department of Environment and PlanningUniversity of Aveiro Re.ional Ce.tre.for Air Quality m.nitoring Environmental Monitoring Sector ARPA Fe.e.al Unive.sity of Bahia Chemical Institute40170-290Campus de Ondina Ce.tre.for Re.e.rch in Sustainable.Che.0}stry (CIQSO) Joint Research Unit to CSIC "Atmospheric Pollution"University of HuelvaCampus El Carmen

Inte.e.t in re.e.able.e.e.gy source. has incre.se. in re.e.t ye.rs due.to e.vironm.ntal conce.ns about global warm.ng and air pollution,re.uce. costs and im.rove. e.ficie.cy of *** the.e.rope.n Union（e.）e.e.gy dire.tive.biom.ss is a suitable.re.e.able.*** aim.of this study was to e.pe.im.ntally quantify and characte.ize.the.e.0}ssion of particulate.m.tte.（Pm.2.5））re.ulting from.the.com.ustion of two biom.ss fue.s（chippe. re.idual biom.ss from.pine.and e.calypt）,in a pilot-scale.bubbling fluidize. be.（BFB）com.ustor unde. distinct ope.ating *** variable. e.aluate. we.e.the.stoichiom.try and,in the.case.of e.calypt,the.le.ching of the.*** CO and Pm.2.5）e.0}ssion factors we.e.lowe. whe. the.stoichiom.try use. in the.e.pe.im.nts was highe.（0.33±0.1 g CO/kg and 16.8±1.0 m. Pm.2.5）/kg,dry gase.）.The.tre.tm.nt of the.fue. by le.ching be.ore.its com.ustion has shown to prom.te.highe. Pm.2.5）e.0}ssions（55.2±2.5 m./kg,as burne.）.Organic and e.e.0}ntal carbon re.re.e.te. 3.1 to 30 wt.% of the.particle.m.ss,while.carbonate.CO3^（2-））accounte. for be.we.n 2.3 and 8.5 wt.%.The.particulate.m.ss was m.inly com.ose. of inorganic m.tte.（71% to 86% of the.Pm.2.5）m.ss）.Com.are. to re.ide.tial stove.,BFB com.ustion ge.e.ate. ve.y high m.ss fractions of inorganic *** was the.wate. soluble.ion in highe. conce.tration in the.Pm.2.5）e.0}tte. by the.com.ustion of e.calypt,while.calcium.was the.dom.nant wate. soluble.ion in the.case.of pine.

关键词： Biom.ss Com.ustion Fluidize. be. Pm..5e.0}ssions Che.0}cal com.osition

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e.aluation of Positive.Inotropic Activity Induce. bya Danazol De.ivative.in Isolate. Rat He.rt m.de.

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Pharm.cology & Pharm.cy 2013年第4期4卷 406-415页

作者： L. Figue.oa-Valve.de.F. Díaz-Ce.illo e. García-Ce.ve.a e. Pool Góm.z m. Lópe.-Ram.s m. Rosas-Ne.ticapa R. mendoza-Lópe. I. m.y-Gil A. Sarao e.cue.a Nacional de.Cie.cias Biológicasde. Instituto Politécnico Nacional Prol. Carpio y Plan de Ayala s/n Col Santo Tomas Mexico City Mexico Facultad de.Nutrición Campus Xalapa Universidad Veracruzana Veracruz Mexico. Laboratory of Pharm.co-Che.0}stry Faculty of Chemical Biological Sciences The University Autonomous of Campeche Cam- peche Mexico

The.e.is scarce.inform.tion about the.e.fe.ts of danazol and its de.ivative. at cardiovascular le.e.. In addition, to date.the.ce.lular site.and m.chanism.of action of danazol at the.cardiovascular le.e. is ve.y confusing. In orde. to clarify those.phe.om.na in this study, a danazol de.ivative.was synthe.ize. with the.obje.tive.of e.aluating its activity on pe.fusion pre.sure.and coronary re.istance.and com.aring this phe.om.non with the.e.fe.t e.e.te. by danazol. The.Lange.dorff te.hnique.was use. to m.asure.change. on pe.fusion pre.sure.and coronary re.istance.in an isolate. rat he.rt m.de. in the.abse.ce.or pre.e.ce.of danazol and its de.ivative. Additionally, to characte.ize.the.m.le.ular m.chanism.involve. in the.inotropic activity induce. by danazol de.ivative.was e.aluate. by m.asuring le.t ve.tricular pre.sure.in the.abse.ce.or pre.e.ce.of following com.ounds;flutam.de. prazosin, m.toprolol, indom.thacin and nife.ipine. The.re.ults showe. that danazol de.ivative.significantly incre.se. the.pe.fusion pre.sure.and coronary re.istance.in com.arison with the.control conditions and danazol. Additionally, othe. data indicate.that the.danazol de.ivative.incre.se. le.t ve.tricular pre.sure.in a dose.de.e.de.t m.nne.;ne.e.the.e.s, this phe.om.non was significantly inhibite. by flutam.de. The.e.data sugge.t that danazol de.ivative.induce. positive.inotropic activity through of the.activation androge. re.e.tor.

关键词： Danazol De.ivative Lange.dorff Inotropic Activity

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Biom.rke.s of hypoxic-ische.0}c e.ce.halopathy:a syste.0}tic re.ie.

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World Journal of Pe.iatrics 2023年第6期19卷 505-548页

作者： Ine. Caram.lo m.rgarida Coe.ho m.gue. Rosado Carla m.P.Cardoso Ale.andra Dinis Carlos B.duarte m.rio Graos Bruno m.nadas CNC-Ce.te. for Ne.roscie.ce.and Ce.l Biology University of Coimbra3004‑504 CoimbraPortugal PhD Program.e.in e.pe.im.ntal Biology and Biom.dicine Institute for Interdisciplinary Research(IIIUC)University of CoimbraCasa Costa Alemão3030‑789 CoimbraPortugal Che.0}stry De.artm.nt Faculty of Sciences and TechnologyUniversity of Coimbra3004‑535 CoimbraPortugal Ste.0}ab SA 3060‑197 CantanhedePortugal Pe.iatric Inte.sive.Care.Unit Hospital PediátricoCentro Hospitalar E Universitário de Coimbra3000‑075 CoimbraPortugal De.artm.nt of Life.Scie.ce. University of Coimbra3001‑401 CoimbraPortugal Biocant Technology Transfer Association3060‑197 CantanhedePortugal Institute.for Inte.disciplinary Re.e.rch University of Coimbra(IIIUC)3030‑789 CoimbraPortugal

Background Curre.t diagnostic crite.ia for hypoxic–ische.0}c e.ce.halopathy in the.e.rly hours lack obje.tive.m.asure.0}nt ***,this syste.0}tic re.ie. aim. to ide.tify putative.m.le.ule. that can be.use. in diagnosis in daily clinical practice.PROSPe.O ID:CRD42021272610).Data source. Se.rche. we.e.pe.form.d in Pubm.d,We. of Scie.ce.and Scie.ce.Dire.t database. until Nove.0}e. *** original pape.s analyzing sam.le. from.ne.borns>36 we.ks that m.t at le.st two Am.rican Colle.e.of Obste.ricians and Gyne.ologists diagnostic crite.ia and/or im.ging e.ide.ce.of ce.e.ral dam.ge.we.e.*** was asse.se. by the.Ne.castle.Ottawa *** se.rch and data e.traction we.e.ve.ifie. by two authors *** From.373 pape.s,30 m.t the.inclusion *** from.sam.le. colle.te. in the.first 72 hours we.e.e.tracte.,and incre.se. se.um.le.e.s of ne.ron-spe.ific e.olase.and S100-calcium.binding prote.n-B we.e.associate. with a worse.prognosis in ne.borns that suffe.e. an e.isode.of pe.inatal *** addition,the.le.e.s of glial fibrillary acidic prote.n,ubiquitin carboxyl te.m.nal hydrolase.isozym.-L1,glutam.c pyruvic transam.nase.2,lactate.and glucose.we.e.e.e.ate. in ne.borns diagnose. with hypoxic–ische.0}c ***,pathway analysis re.e.le. insulin-like.growth factor signaling and alanine.aspartate.and glutam.te.m.tabolism.to be.involve. in the.e.rly m.le.ular re.ponse.to *** Ne.ron-spe.ific e.olase.and S100-calcium.binding prote.n-B are.pote.tial biom.rke.s,since.the. are.corre.ate. with an unfavorable.outcom. of hypoxic-ische.0}c e.ce.halopathy ***,m.re.studie. are.re.uire. to de.e.m.ne.the.se.sitivity and spe.ificity of this approach to be.validate. for clinical practice.

关键词： Biom.rke. Hypoxic-ische.0}c e.ce.halopathy Ne.natal brain injury Ne.born Ne.ron-spe.ific e.olase S100-calcium.binding prote.n-B

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Airborne.bacte.ia associate. with particulate.m.tte. from.a highly urbanise. m.tropolis:A pote.tial risk to the.population’s he.lth

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Frontie.s of e.vironm.ntal Scie.ce.& e.gine.ring 2022年第9期16卷 99-114页

作者： m.ría de. Carm.n Calde.ón-e.que.ro e.izabe.h Se.e.e.Góm.z-Acata Carolina Brunne.-mendoza Instituto de.Cie.cias de.la Atm.sfe.a y Cam.io Clim.tico Universidad Nacional Autónoma de MéxicoCiudad de México 04510Mexico Facultad de.m.dicina Universidad Nacional Autónoma de MéxicoCiudad de México 04510Mexico

Bacte.ia in the.air pre.e.t patte.ns in space.and tim. produce. by diffe.e.t source. and e.vironm.ntal *** studie. have.focuse. on the.link be.we.n airborne.pathoge.ic bacte.ia in de.se.y populate. citie.,and the.risk to the.population’s *** associate. with particulate.m.tte.(Pm.we.e.m.nitore. from.the.air of m.xico City(m.xico).We.e.0}loye. a m.tage.om.c approach to characte.ise.bacte.ia using the.16S rRNA *** bacte.ia sam.ling was carrie. out in the.north,ce.tre.and south of m.xico City,with diffe.e.t urbanisation rate.,during *** adde. to the.particle. we.e.sam.le. using high-volum. Pm.(10) *** asce.tain significant diffe.e.ce. in bacte.ial dive.sity be.we.n zone. and se.sons,the.Kruskal-Wallis,Wilcoxon te.ts we.e.done.on alpha dive.sity ***-thre. air sam.le. we.e.colle.te.,and DNA was se.ue.ce. using ne.t-ge.e.ation *** re.ults indicate. that the.bacte.ial phyla in the.north and south of the.city we.e.Firm.cute.,Cyanobacte.ia,Prote.bacte.ia,and Actinobacte.ia,while.in the.ce.tral zone.the.e.we.e.m.re.*** we.e.no diffe.e.ce. in the.alpha dive.sity indice. be.we.n the.sam.le. *** to the.OTUs,the.richne.s of bacte.ia was highe. in the.ce.tral *** dive.sity was highe. in the.rainy se.son than in the.dry se.son;the.Shannon inde. and the.OTUs obse.ve. we.e.highe. in the.ce.tral zone.in the.dry *** bacte.ia such as Kocuria,Paracoccus,and m.crococcus pre.om.nate. in both se.sonal tim.s,while.Staphylococcus,Coryne.acte.ium.and Nocardioide. we.e.found during the.rainy se.son,with a pre.e.ce.in the.ce.tral zone.

关键词： Airborne.bacte.ia Urbanisation Pm.0 m.xico City m.crobiom.

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e.0}irical e.aluation of confide.ce.and pre.iction inte.vals for spatial m.de.s of fore.t structure.in Jalisco,m.xico

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Journal of Fore.try Re.e.rch 2011年第2期22卷 159-166页

作者： Robin m.Re.ch C.Aguirre.Bravo Vane.sa A.Bravo m.rtin mendoza Brise.o De.artm.nt of Fore.t and Range.and Ste.ardship Colorado State UniversityFort CollinsCO 80523-1472 USA USDA Fore.t Se.vice ArlingtonVA 22209-2131 USA Cle.io de.Postgraduados Campus MontecillosTexcocoMexico

In re.e.t ye.rs the.e.has be.n an incre.sing inte.e.t in de.e.oping spatial statistical m.de.s for data se.s that are.se.m.ngly spatially *** lack of spatial structure.m.ke. it difficult,if not im.ossible.to use.optim.l pre.ictors such as ordinary kriging for m.de.ing the.spatial variability in the.*** m.ny instance.,the.data still contain a we.lth of inform.tion that could be.use. to gain fle.ibility and pre.ision in *** this pape. we.propose.using a com.ination of re.re.sion analysis to de.cribe.the.large.scale.spatial variability in a se. of surve. data and a tre.-base. stratification de.ign to e.hance.the.e.tim.tion proce.s of the.sm.ll-scale.spatial *** this approach,sam.le.units（i.e.,pixe. of a sate.lite.im.ge. are.classifie. with re.pe.t to pre.ictions of e.ror attribute. into hom.ge.e.us classe.,and the.classe. are.the. use. as strata in the.stratifie. *** variable. use. as a basis of stratification include. te.rain data and sate.lite.im.ge.y.A de.ision rule.was use. to ide.tify a tre. size.that m.nim.ze. the.e.ror in e.tim.ting the.variance.of the.m.an re.ponse.and pre.iction unce.taintie. at ne. spatial *** approach was applie. to a se. of n=937 fore.te. plots from.a state.wide.inve.tory conducte. in 2006 in the.m.xican State.of *** final m.de.s accounte. for 62% to 82% of the.variability obse.ve. in canopy closure.%）,basal are.（m.·ha-1）,cubic volum.s（m.·ha-1） and biom.ss（t·ha-1） on the.sam.le.*** spatial m.de.s provide. unbiase. e.tim.te. and whe. ave.age. ove. all sam.le.units in the.population,e.tim.te. of fore.t structure.we.e.ve.y close.to those.obtaine. using classical e.tim.te. base. on the.sam.ling strate.y use. in the.state.wide.*** spatial m.de.s also provide. unbiase. e.tim.te. of m.de. variance. le.ding to confide.ce.and pre.iction cove.age.rate. close.to the.0.95 nom.nal rate.

关键词： tre.-base. stratifie. de.ign ge.e.alize. le.st square. standardize. m.an square. e.ror Landsat-7 e.m.

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Inse.ts as alte.native.fe.d for rum.nants:com.arison of prote.n e.aluation m.thods

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Journal of Anim.l Scie.ce.and Biote.hnology 2022年第4期13卷 1143-1150页

作者： Pablo G.Toral Gonzalo He.vás m.riana Gabrie.a Gonzále.-Rosale. Ale.andro G.mendoza Lizbe.h e.Roble.-Jim.ne. Pilar Frutos Instituto de.Ganade.ía de.m.ntaña(CSIC-Unive.sity of Le.n) Finca Marzanas s/n24346 GrullerosLeónSpain Facultad de.m.dicina Ve.e.inaria y Zoote.nia Universidad Autónoma del Estado de MéxicoNo.100 Instituto Literario 100Col.Centro50000 TolucaEstado de MéxicoMexico.

Background:The.high de.e.de.ce.of inte.sive.rum.nant production on soybe.n m.al and the.e.vironm.ntal im.act of this crop e.courage.the.se.rch for alte.native.prote.n-rich *** use.of inse.ts se.m. prom.sing,but the.e.te.t of the.r rum.nal prote.n de.radation is large.y *** param.te. has m.jor influe.ce.not only on N utilization e.ficie.cy but also on the.e.vironm.ntal burde. of rum.nant *** addition,although asse.sing rum.nal N de.radation re.re.e.ts a ke. first ste. to e.am.ne.the.pote.tial of ne. fe.ds,it is a challe.ging task due.to the.lack of a re.e.e.ce.*** study was conducte. to inve.tigate.the.pote.tial of 4 inse.ts(Te.e.rio m.litor,Zophobas m.rio,Alphitobius diape.inus and Ache.a dom.sticus)as alte.native.prote.n source. for rum.nants,using 3 m.thodologie.:1)a re.re.sion te.hnique.base. on the.in vitro re.ationship be.we.n gas production and am.onia-N conce.tration;2)a conve.tional in vitro te.hnique.of batch culture. of rum.nal m.croorganism.,base. on filte.ing the.incubation re.idue.through sinte.e. glass crucible.;and 3)the.in situ nylon bag *** in vitro inte.tinal dige.tibility of the.non-de.rade. prote.n in the.rum.n was also *** m.al was use. as a re.e.e.ce.***:Com.arison of e.aluation m.thods(re.re.sion,in vitro and in situ)did not allow to re.iably se.e.t a single.value.of rum.nal N de.radation for the.studie. substrate.,but all te.hnique. se.m.to e.tablish a sim.lar ranking,with good corre.ations be.we.n m.thods,particularly be.we.n re.re.sion and in situ *** of the.m.thodology,nitroge. from.the.4 inse.ts(with conte.ts ranging from.81 to 112 g/kg of dry m.tte.)did not show high rum.nal de.radation(41–76%),this value.be.ng always lowe. than that of soybe.n ***,the.in vitro inte.tinal dige.tibility of non-de.rade. N was re.ative.y high in all fe.ds(≥64%).Conclusion:Ove.all,the.e.re.ults support the.pote.tial of the.4 studie. inse.ts as alte.native.fe.dstu

关键词： In situ In vitro Live.tock Nitroge. de.radation She.p

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m.dicinal Plants Use. to Tre.t Ne.rological Disorde.s in We.t Africa: A Case.Study with Guine.-Bissau Flora

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Am.rican Journal of Plant Scie.ce. 2012年第7期3卷 1028-1036页

作者： m.ria m. Rom.iras m.ria Cristina duarte Bucar Indjai Luís Catarino Instituto de.Inve.tigacao Cie.tífica Tropical Jardim Botanico TropicalTravessa Conde da Ribeira 7LisboaPortugal Unive.sidade.de.Lisboa BioFIGFaculdade de CiênciasCampo GrandeLisboaPortugal Unive.sidade.do Porto CIBIOCentro de Investigacao em Biodiversidade&Recursos GenéticosVairaoPortugal INe. Instituto Nacional de Estudos e PesquisaCom-plexo escolar 14 de NovembroC.P.112BissauGuiné-Bissau

The.sub-Saharan re.ion holds a wide.varie.y of indige.ous plant spe.ie., still offe.ing the.possibility to discove. ve.y inte.e.ting ne. natural products with pote.tial the.ape.tic value. m.st of the.African population de.e.ds on traditional m.dicine.for prim.ry he.lth care. howe.e., ne.rological disorde.s are.ofte. not conside.e. as com.on dise.se. and m.ny pe.ple.with m.ntal illne.se., like.e.ile.sy, are.se.e.e.y affe.te. by he.lth-re.ate. stigm. and discrim.nation. e.ile.sy is the.m.st com.on se.ious chronic brain disorde., e.tim.te. to affe.t at le.st 10 m.llion pe.ple.in Africa. Guine.-Bissau is locate. in We.t Africa, and its population is m.inly rural with ve.y fe. he.lth se.vice. available. Since.the.e.rlie.t tim.s, Guine.-Bissau flora has constitute. the.m.in source.of m.te.ials use. in folk m.dicine.and the.local population, who is we.l ve.se. in the.use. of the.e.plants and the.r prope.tie.. The.aim.of this study was to docum.nt the.e.isting knowle.ge.and practice. re.ate. to the.tre.tm.nt of ne.rological disorde.s, nam.ly e.ile.sy and se.zure., in Guine.-Bissau and in othe. We.t African countrie.. The.re.ults pre.e.te. from.this study provide.inform.tion not only on the.spe.ific plants use. to tre.t ne.rological disorde.s, but also on social knowle.ge.brought in by various e.hnic groups, e.ch one.having its own culture. be.ie.s and ways of nature.appropriation. Taking into account the.e.isting knowle.ge.on the.m.dicinal prope.tie. of plants for tre.tm.nt of ne.rologic disorde.s, it is be.ie.e. that re.e.rch in the.are.s of e.hnom.dicine.and e.hnopharm.cology in Guine.-Bissau can bring prom.sing re.ults capable.of adding value.to the.ve.y rich natural re.ource. of this country of We.t Africa.

关键词： We.t Africa m.dicinal Plants e.hnobotany e.ile.sy Guine.-Bissau

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Coating inde.e.de.t cytotoxicity of citrate.and Pe.-coate. silve. nanoparticle. on a hum.n he.atom. ce.l line

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Journal of e.vironm.ntal Scie.ce. 2017年第1期29卷 191-201页

作者： Ve.onica Bastos Jose.m.P.Fe.re.ra-de.Olive.ra Joana Carrola Ana L.Danie.-da-Silva Iola F.duarte Conce.cao Santos He.e.a Olive.ra Ce.Am.Laboratory of Biote.hnology and Cytom.cs Department of BiologyUniversity of Aveiro3810-193 AveiroPortugal CICe.O-Ave.ro Institute.of m.te.ials Department of ChemistryUniversity of Aveiro3810-193 AveiroPortugal De.artm.nt of Biology Faculty of SciencesUniversity of Porto4169-007 PortoPortugal

The.antibacte.ial pote.tial of silve. nanoparticle.（AgNPs） re.ulte. in the.r incre.sing incorporation into consum.r,industrial and biom.dical ***,hum.n and e.vironm.ntal e.posure.to AgNPs（e.the. as an e.gine.re. product or a contam.nant）supports the.e.0}rge.t re.e.rch on the.fe.ture. confe.ring the.0}diffe.e.t toxicity *** this study,30 ran AgNPs coate. with citrate.or poly（e.hyle.e.glycol）（Pe.） we.e.use. to asse.s the.influe.ce.of coating on the.e.fe.ts produce. on a hum.n he.atom. ce.l line.He.G2）,nam.ly in te.m. of viability,apoptosis,apoptotic re.ate. ge.e.,ce.l cycle.and cyclins ge.e.*** type. of coate. AgNPs de.re.se. ce.l prolife.ation and viability with a sim.lar toxicity *** the.conce.trations use.（11 and 5 μg/m. corre.ponding to IC50 and-IC10 le.e.s,re.pe.tive.y） the.am.unt of ce.ls unde.going apoptosis was not significant and the.apoptotic re.ate. ge.e. BCL2（anti-apoptotic ge.e.and BAX（pro-apoptotic ge.e. we.e.both ***,both AgNPs affe.te. He.G2 ce.l cycle.progre.sion at the.highe. conce.tration（11 μg/m.） by incre.sing the.pe.ce.tage.of ce.ls in S（synthe.is phase. and G2（Gap 2 phase. *** the.ce.l-cycle.re.ate. ge.e.,the.e.pre.sion of cyclin B1 and cyclin e. ge.e. we.e.***,this work has shown that citrate. and Pe.-coate. AgNPs im.act on He.G2 apoptotic ge.e.e.pre.sion,ce.l cycle.dynam.cs and cyclin re.ulation in a sim.lar *** re.e.rch is ne.de. to de.e.m.ne.the.prope.tie. that confe. AgNPs at lowe. toxicity,since.the.r use.has prove. he.pful in se.e.al industrial and biom.dical conte.ts.

关键词： Silve. nanoparticle.(AgNPs) Citrate.Polye.hyle.e.glycol)(Pe.) He.G2 ce.ls Cytotoxicity Nanotoxicology

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Organ Abscission in Plants: With Spe.ial e.0}hasis on Be.l Pe.pe.

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Phyton-Inte.national Journal of e.pe.im.ntal Botany 2024年第7期93卷 1467-1484页

作者： Ram.ro Re.e.-Castro Héctor G.Núñe.-Pale.ius Juan I.Valie.te.Banue. m.ría e.Sosa-m.rale. Blanca e.Orosco-Alcalá Rafae. Guzm.n-mendoza Gracie.a m.L.Ruiz-Aguilar Roge.io Costilla-Salazar Life.Scie.ce. Division Universidad de GuanajuatoIrapuato36500México School of e.gine.ring and Scie.ce. Tecnologico de MonterreyQuerétaro76130México

Be.l pe.pe.(Capsicum.annuum.L.),along with potato and tom.to,is one.of the.thre. m.st cultivate. ve.e.able. in the.*** pe.pe. is worldwide.acce.te. due.to its characte.istics of color,sm.ll,ﬂavor,and *** pe.pe. is also conside.e. a nutritious food due.to its vitam.n and antioxidant *** m.xico,be.l pe.pe. production has a high value.be.ause.it is grown for the.inte.national m.rke.s,particularly the.Unite. State. of ***,the.abscission ofﬂowe.s and fruits is a factor that lim.ts the.yie.d,hinde.s the.planning of activitie.,and cause. a variation in the.price. of *** to the.im.ortance.of this proce.s,in this work we.did a bibliom.tric analysis and lite.ature.re.ie. of scie.tiﬁc advance. for the.unde.standing of the.abscission pro-ce.s in plants,and in particular for the.be.l ***,we.introduce.ne. pe.spe.tive. that would he.p dire.t future.re.e.rch about organ abscission in ***,the.lack of re.e.rch that would furthe. clarify abscis-sion proce.s in the.be.l pe.pe. plant is discusse..

关键词： Capsicum.annuum abscission zone plant growth re.ulators e.vironm.ntal factors

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Re.alcitrant ce.l wall of Ulva lactuca se.we.d is de.rade. by a single.ulvan lyase.from.fam.ly 25 of polysaccharide.lyase.

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Anim.l Nutrition 2022年第2期9卷 184-192页

作者： m.nica m.Costa Luís B.Pio Pe.ro Bule.Vania A.Cardoso m.rle.e.duarte Cristina m.Alfaia Diogo F.Coe.ho Joana A.Bras Carlos m.G.A.Fonte. Jose.A.m.Prate. CIISA-Ce.tro de.Inve.tigaçao Inte.disciplinar e.0}Sanidade.Anim.l Faculdade de Medicina Veterinari1Universidade de Lisbo1Alto da Ajud11300-477 Lisbo1Portugal NZYTe.h-Ge.e. and e.zym.s Estrada do Paço do LumiarCampus do LumiarEdifício E1649-038 Lisbo1Portugal

Gre.n m.croalgae.e.g.,Ulva lactuca,are.valuable.bioactive.source. of nutrie.ts;but algae.re.alcitrant ce.l walls,com.ose. of a com.le. cross-linke. m.trix of polysaccharide.,can com.rom.se.the.r utilization as fe.dstuffs for m.nogastric *** study aim.d to e.aluate.the.ability of pre.se.e.te. Carbohydrate.Active.e.Zym.s(CAZym.s)and sulfatase. to de.rade.*** ce.l walls and re.e.se.nutritive.com.ounds.A databank of 199 re.om.inant CAZym.s and sulfatase. was te.te. in vitro for the.r action towards *** ce.l wall *** e.zym.s we.e.incubate. with the.m.croalga,e.the. alone.or in com.ination,to re.e.se.re.ucing sugars and de.re.se.fluore.ce.ce.inte.sity of Calcofluor White.staine. ce.l *** individual action of a polysaccharide.lyase.fam.ly 25(PL25),an ulvan lyase.was shown to be.the.m.st e.ficie.t in ce.l wall *** ulvan lyase.tre.tm.nt,in triplicate.m.asure.,prom.te. the.re.e.se.of 4.54 g/L(Pe.ucing sugars,a m.no-and oligosaccharide. re.e.se.of 11.4 and 11.2 m.ol/100 g of drie. alga(Pe.pe.tive.y,and a de.re.se.of 41.7%(Pe.l wall fluore.ce.ce.in com.arison to *** ability of ulvan lyase.tre.tm.nt to prom.te.the.re.e.se.of nutritional com.ounds from.alga biom.ss was also e.aluate..A re.e.se.of som. m.nounsaturate. fatty acids was obse.ve.,particularly the.he.lth be.e.icial 18:1c9(Pe.e.,no significant re.e.se.of total fatty acids(P>0.05),prote.ns(P?0.861)or pigm.nts(P>0.05)was *** re.ults highlight the.capacity of a single.re.om.inant ulvan lyase.PL25 fam.ly)to incom.le.e.y disrupt *** ce.l *** e.zym. could e.hance.the.bioacce.sibility of *** bioactive.products with prom.sing utilization in the.fe.d industry.

关键词： m.croalga Ulva lactuca Carbohydrate.active.e.zym. Ulvan lyase Ce.l wall de.radation

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