Insects as alternative feed for ruminants:comparison of protein evaluation methods

作     者：Pablo G.Toral Gonzalo Hervás Mariana Gabriela González-Rosales Alejandro G.Mendoza Lizbeth E.Robles-Jiménez Pilar Frutos Pablo G.Toral;Gonzalo Hervás;Mariana Gabriela González-Rosales;Alejandro G.Mendoza;Lizbeth E.Robles-Jiménez;Pilar Frutos

作者机构：Instituto de Ganadería de Montaña(CSIC-University of León)Finca Marzanas s/n24346 GrullerosLeónSpain Facultad de Medicina Veterinaria y ZootecniaUniversidad Autónoma del Estado de MéxicoNo.100 Instituto Literario 100Col.Centro50000 TolucaEstado de MéxicoMexico. 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2022年第13卷第4期

页      面：1143-1150页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：conducted within the framework of project PIE 202040E100 (CSIC, Spanish National Research Council) Pablo G. Toral benefited from a Ramón y Cajal research contract (RYC-2015-17230), from the Spanish Ministry of Economy and Competitiveness (MINECO), co-funded by the European Social Fund Lizbeth E. Robles-Jiménez was granted a SEGIB-Fundación Carolina fellowship 

主　　题：In situ In vitro Livestock Nitrogen degradation Sheep 

摘      要：Background:The high dependence of intensive ruminant production on soybean meal and the environmental impact of this crop encourage the search for alternative protein-rich *** use of insects seems promising,but the extent of their ruminal protein degradation is largely *** parameter has major influence not only on N utilization efficiency but also on the environmental burden of ruminant *** addition,although assessing ruminal N degradation represents a key first step to examine the potential of new feeds,it is a challenging task due to the lack of a reference *** study was conducted to investigate the potential of 4 insects(Tenebrio molitor,Zophobas morio,Alphitobius diaperinus and Acheta domesticus)as alternative protein sources for ruminants,using 3 methodologies:1)a regression technique based on the in vitro relationship between gas production and ammonia-N concentration;2)a conventional in vitro technique of batch cultures of ruminal microorganisms,based on filtering the incubation residue through sintered glass crucibles;and 3)the in situ nylon bag *** in vitro intestinal digestibility of the non-degraded protein in the rumen was also *** meal was used as a reference ***:Comparison of evaluation methods(regression,in vitro and in situ)did not allow to reliably select a single value of ruminal N degradation for the studied substrates,but all techniques seem to establish a similar ranking,with good correlations between methods,particularly between regression and in situ *** of the methodology,nitrogen from the 4 insects(with contents ranging from 81 to 112 g/kg of dry matter)did not show high ruminal degradation(41–76%),this value being always lower than that of soybean ***,the in vitro intestinal digestibility of non-degraded N was relatively high in all feeds(≥64%).Conclusion:Overall,these results support the potential of the 4 studied insects as alternative feedstu