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The Classical and Regulatory Functions of C1q in Immunity and Autoimmunity

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Cellular & Molecular Immunology 2008年第1期5卷 9-21页

作者： Jinhua Lu Boon King Teh linda Wang Yinan Wang Yen Seah Tan min Chern Lai Kenneth B. M. Reid Department of Microbiology Yong Loo Lin School of Medicine and 2NUS Immunology Program National University of Singapore Blk MD4 5 Science Drive 2 Singapore 117597 MRC Immunochemistry Unit Department of Biochemistry University of Oxford South Parks Road Oxford OXI 3QU UK

A classical function of Clq is to bind immune complexes and initiate complement activation producing membrane lytic complexes, opsonins and anaphylatoxins. This classical pathway of complement activation is also elicited when Clq binds some other ligands. Besides complement activation, Clq also regulates cell differentiation, adhesion, migration, activation and survival. Clq deficiency is associated with autoimmunity as well as increased susceptibility to infections. In this article, we discuss the basic properties of Clq, its expression, and classical and regulatory functions. Cellular ＆ Molecular Immunology.

关键词： C1q macrophage dendritic cell apoptotic cell autoimmunity

Esophagogastroduodenoscopy with conscious sedation does not interfere with catheter-based 24-h pH monitoring

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World Journal of Gastroenterology 2013年第11期19卷 1805-1810页

作者： Yung-Kuan Tsou Jau-min lien Chin-Kuo Chen Cheng-Hui lin Hsing-Yu Chen Mu-Shien Lee Department of Gastroenterology and Hepatology Chang Gung Memorial HospitalChang Gung University College of MedicineTaoyuan 333Taiwan Department of Otolaryngology Chang Gung Memorial HospitalChang Gung University College of MedicineTaoyuan 333Taiwan Center for Traditional Chinese Medicine Chang Gung Memorial HospitalChang Gung University College of MedicineTaoyuan 333Taiwan

AIM:To investigate the impact of esophagogastroduodenoscopy with conscious sedation on the subsequent 24-h catheter-based pH ***:Fifty patients with extra-esophageal symptoms of gastroesophageal reflux disease undergoing ambulatory dual-probe 24-h pH monitoring were enrolled from March 2010 to August *** of the data were collected prospectively and analyzed ***-six patients(72%,group A) underwent pH monitoring shortly after esophagogastroduodenoscopy(EGD) with conscious sedation,and 14 patients(28%,group B) underwent pH monitoring without conscious *** 24-h pH data from two time periods were analyzed:the first 4 h(Period Ⅰ) and the remaining time of the study(Period Ⅱ).RESULTS:The mean age of the patients was 49.6 ± 12.5 years;20 patients(40%) were *** baseline data,including age,sex,body mass index,reflux esophagitis,the Reflux Symptom Index,and the Reflux Findings Score,were comparable between the two *** percentage of total time with a pH < 4 and the frequency of acid reflux during Period Ⅰ were not significantly different between the two groups,as measured using both pharyngeal(0.03% ± 0.10% vs 0.07% ± 0.16%,P = 0.32;and 0.07 ± 0.23 episodes/h vs 0.18 ± 0.47 episodes/h,P = 0.33,respectively) and esophageal probes(0.96% ± 1.89% vs 0.42% ± 0.81%,P = 0.59;and 0.74 ± 1.51 episodes/h vs 0.63 ± 0.97 episodes/h,P = 0.49,respectively).The percentage of total time with a pH < 4 and the frequency of acid reflux were also not significantly different between Periods I and Ⅱ in group A patients,as measured using both pharyngeal(0.03% ± 0.10% vs 0.23% ± 0.85%,P = 0.21;and 0.07 ± 0.23 episodes/h vs 0.29 ± 0.98 episodes/h,P = 0.22,respectively) and esophageal probes(0.96% ± 1.89% vs 1.11% ± 2.57%,P = 0.55;and 0.74 ± 1.51 episodes/h vs 0.81 ± 1.76 episodes/h,P = 0.55,respectively).CONCLUSION:EGD with conscious sedation does not interfere with the results of subsequent 24-h pH monitoring in patients with extra-esophageal

关键词： Esophagogastroduodenoscopy Conscious sedation pH monitoring Gastroesophageal reflux disease Extraesophageal symptoms

Role of JAK2/STAT3 Pathway in IL-6-Induced Activation of Human Proximal Tubular Epithelial Cells

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器官移植内科学杂志 2009年第4期4卷 181-186页

作者： linda Zhan Hequn Zou Lixin Wei lin Chen Wenying Zhou Department of Nephrology The 5th Affiliated Hospital of Sun Yat-sen University Zhuhai 519000 China Department of Nephrology and Rheumatology The 3rd Affiliated Hospital of Southern Medical UniversityGuangzhou 510630 China

Objective To study the role of JAK2/STAT3 in IL-6-induced activation of human proximal tubular epithelial cells (HK-2 cells). Methods Cell proliferation was measured by MTT assay. HK-2 cells growing in RPMI 1640 medium containing 10% fetal bovine serum were cultured for 24h in serum-free medium after 60%-80% cells pasted on the wail, and then cultured with IL-6 at designated concentration for designated periods. The expression of a-SMA was assessed by Western blot after the induction of IL-6 at 25 ng/mL concentrations for a period of 24h, 48h, 72h respectively. The expression of P-STAT3 was detected with Western blot after the induction of IL-6 at 25 ng/mL concentration for a period of 0, 15, 30, 60, 120 min respectively and at the concentrations of 0, 5, 10, 25, 50 ng/mL for 30 min. The cells were pre-incubated with 100 uM AG490 for 4h and then stimulated with 25 ng/mi IL-6 for 30 min and 48h respectively, The expression of P-STAT3, P-JAK2 andα-SMA was determined by Western blot. Results Incubation for 48 hours with 10-50 ng/mL rIL-6 caused concentration- and time-dependent stimulation of HK-2 cells proliferation, IL-6 induced the expression ofα-SMA. IL-6 rapidly induced the phosphorylation of STAT3 in a dose- and time-dependent manner with a peak expression at 30 min. 100 IxM AG490, a specific JAK2 inhibitor, partially inhibited STAT3, JAK2 activation and a-SMA production. Conelusion It is suggested that JAK2/STAT3 pathway partially mediate IL-6-induced et-SMA production in HK-2 cells. It is implicated that JAK2/STAT3 signaling pathway might play a role in the development of tubulointerstitial fibrosis.

关键词：器官移植术临床医学 JAK2/STAT3

Role of JAK2/STAT3 Pathway in IL-6-Induced Activation of Human Proximal Tubular Epithelial Cells

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器官移植内科学杂志 2008年第2期3卷 59-63页

作者： Hequn Zou linda Zhan Lixin Wei lin Chen Wenying Zhou Department of Nephrology The 5th Affiliated Hospital of Sun Yat-sen University Zhuhai 519000 China

Objective To study the role of JAK2/STAT3 in IL-6-induced activation of human proximal tubular epithelial cells (HK-2 cells). Methods Cell proliferation was measured by MTT assay. HK-2 cells growing in RPMI 1640 medium containing 10% fetal bovine serum were cultured for 24h in serum-free medium after 60%-80% cells pasted on the wall, and then cultured with IL-6 at designated concentration for designated periods. The expression ofα-SMA was assessed by Western blot after the induction of IL-6 at 25 ng/mL concentrations for a period of 24h, 48h, 72h respectively. The expression of P-STAT3 was detected with Western blot after the induction of IL-6 at 25 ng/mL concentration for a period of 0, 15, 30, 60, 120 min respectively and at the concentrations of 0, 5, 10, 25, 50 ng/mL for 30 min. The cells were pre-incubated with 100μM AG490 for 4h and then stimulated with 25 ng/ml IL-6 for 30 min and 48h respectively, The expression of P-STAT3, P-JAK2 andα-SMA was determined by Western blot. Results Incubation for 48 hours with 10-50 ng/mL rIL-6 caused concentration- and time-dependent stimulation of HK-2 cells proliferation, IL-6 induced the expression ofα-SMA. IL-6 rapidly induced the phosphorylation of STAT3 in a dose- and time-dependent manner with a peak expression at 30 min. 100μM AG490, a specific JAK2 inhibitor, partially inhibited STAT3, JAK2 activation andα-SMA production. Conclusion It is suggested that JAK2/STAT3 pathway partially mediate IL-6-inducedα-SMA production in HK-2 cells. It is implicated that JAK2/STAT3 signaling pathway might play a role in the development of tubulointerstitial fibrosis.

关键词：上皮细胞人体生理学 HK-2细胞

Rac1 Overexpression is correlated with Epithelial-Mesenchymal Transition and predicts poor prognosis of colon cancer

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Rac1 Overexpression is correlated with Epithelial-Mesenchyma...

Goldilocks principle of minimally invasive surgery for gastric subepithelial tumors

2017年中国肿瘤标志物学术大会暨第十一届肿瘤标志物青年科学家论坛

作者： Yujuan Zhou Longzheng Xia linda Oyang Heran Wang Jingguan lin Shiming Tan Yutong Tian min Su Xia Luo Xiaoyan Chen Hui Wang Qianjin Liao Key Laboratory of Translational Radiation Oncology Hunan ProvinceHunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of MedicineCentral South University

Objective: Rac1 and epithelial-mesenchymal transition(EMT) is an important target in new cancer treatment strategies. We investigated the clinical significance of Rac1 and markers of EMT expression in colon cancer, and their possible correlation with EMT phenotype. Methods: Immunohistochemistry was used to assess the expressions of Rac1, Snail1, Twist1, N-cadherin(Ncad), Vimentin(Vim), and E-cadherin(E-cad) in 126 colon cancer paraffin-embedded specimens and 50 tumor-adjacent normal specimens. The association of Rac1 and EMT markers with clinicopathological characteristics and relationships between the protein levels and progression free survival(PFS) and overall survival(OS) were analyzed. Results: Compared with non-tumorous tissues, Rac1, Snail1, Twist1, N-cad, Vim levels were markedly increased in colon cancer tissues, whereas decreased E-cad levels were observed(P linicopathologic characteristics, multivariateanalysis indicated Rac1, Snail1, Twist1, N-cad, Vim, and E-cad expression respectively could be as an independent prognostic factor in colon cancer. Rac1 expression was positively correlated with Snail1, Twist1, N-cad, Vim levels(P<0.01) and negatively correlated with E-cadherin amounts(P<0.001) in colon cancer tissues. Conclusion: Rac1 may promote colon cancer progression and metastasis by involving EMT program, which may be considered a potential therapeutic target in colon cancer.

关键词： Rac1 colon cancer epithelial-mesenchymal transition prognosis metastasis

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世界胃肠外科杂志:英文版(电子版) 2023年第8期15卷 1629-1640页

作者： Wei-Jung Chang lien-Cheng Tsao Hsu-Heng Yen Chia-Wei Yang Hung-Chi Chang Chew-Teng Kor Szu-Chia Wu Kuo-Hua lin Department of General Surgery Changhua Christian HospitalChanghua 50006TaiwanChina Department of Gastroenterology Changhua Christian HospitalChanghua 50006TaiwanChina General Education Center Chienkuo Technology UniversityChanghua 50006TaiwanChina School of Medicine Chung Shan Medical UniversityTaichung 40201TaiwanChina Big Data Center Changhua Christian HospitalChanghua 50006TaiwanChina Graduate Institute of Statistics and Information Science National Changhua University of EducationChanghua 50007TaiwanChina Transplant Medicine&Surgery Research Center Changhua Christian HospitalChanghua 50006TaiwanChina

BACKGROUND minimally invasive surgery had been tailored to individual cases of gastric subepithelial tumors(SETs)after comparing the clinical outcomes of endoscopic resection(ER),laparoscopic resection(LR),and hybrid *** To study the use of Goldilocks principle to determine the best form of minimally invasive surgery for gastric *** In this retrospective study,194 patients of gastric SETs with high probability of surgical intervention were *** patients underwent tumor resection in the operating theater between January 2013 and December *** patients were divided into two groups,ER or LR,according to the tumor characteristics and the initial intent of *** patients in the ER group required further backup laparoscopic surgery after an incomplete *** patients who had converted open surgery were excluded.A logistic regression model was used to assess the associations between patient characteristics and the likelihood of a treatment *** area under the curve was used to assess the discriminative ability of tumor size and Youden’s index to determine the optimal cut-off tumor *** One-hundred ninety-four patients(100 in the ER group and 94 in the LR group)underwent tumor resection in the operating *** the ER group,27 patients required backup laparoscopic surgery after an incomplete *** patients in the ER group had small tumor sizes and shorter procedure durations while the patients in the LR group had large tumor sizes,exophytic growth,malignancy,and tumors that were more often located in the middle or lower third of the *** groups had similar durations of hospital stays and a similar rate of major postoperative *** patients in the ER group who underwent backup surgery required longer procedures(56.4 min)and prolonged stays(2 d)compared to the patients in the LR group without the increased rate of major postoperative *** optimal cut-off point for the tumor size for l

关键词： Gastric subepithelial tumors Endoscopic resection Laparoscopic resection Tumor size

PHB1 inhibits the proliferation of nasopharyngeal carcinoma cells via inactivation of the NF-κB signaling pathway

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PHB1 inhibits the proliferation of nasopharyngeal carcinoma ...

Secukinumab demonstrates high efficacy and a favorable safety profile over 52 weeks in Chinese patients with moderate to severe plaque psoriasis

2017年中国肿瘤标志物学术大会暨第十一届肿瘤标志物青年科学家论坛

作者： Heran Wang Yujuan Zhou Longzheng Xia linda Oyang Shiming Tan Jingguan lin Yutong Tian min Su Ying Wang Xia Luo Yang Wu Xiaoyan Chen Pan Chen Hui Wang Qianjin Liao Key Laboratory of Translational Radiation Oncology Hunan ProvinceHunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of MedicineCentral South University

Objective: PHB1 has been proved tumor-associated and functions as tumor suppressor in multiple cancer cells. This study aimed to investigate the effects of PHB1 on the proliferation in human nasopharyngeal carcinoma(NPC) cell lines and its mechanisms. Method: PHB1 was stably overexpressed in CNE1 and HNE1 cells. Cell proliferation was assessed by colony formation and CCK8 assay. Cell-cycle distribution and apoptosis were analyzed using flow cytometry. Luciferase reporter assays, immunofluorescent assay, western blot and quantitative RT-PCR were used to detect the NF-κB activity and downstream signaling pathway expression. Results: PHB1 significantly inhibited the proliferation of NPC cell lines CNE1 and HNE1 in vitro. PHB1 induced an apparent cell cycle arrest at the G1/S phase and apoptosis, which was accompanied by an obvious inactivation of the NF-κB signaling pathway. PHB1 led to a remarkable decrease the expression of NF-κB p65, IKKβ, cyclin D1, cyclin-dependent kinase(CDK) 4 and Bcl-2, obviously increased the expression of IκBα, p21, Bax at both mRNA and protein levels in NPC cells. In addition, PHB1 restrained the LPS-induced activation of NF-κB signaling via inhibiting p65 translocation into nuclear. Conclusion: PHB1 could inhibit cell proliferation in vitro via inactivation of the NF-κB signaling pathway. PHB1 maybe serve as a tumor suppressor gene and a potential therapeutic target against NPC.

关键词： Nasopharyngeal carcinoma PHB1 NF-κB cell proliferation signaling pathway

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Chinese Medical Journal 2020年第22期133卷 2665-2673页

作者： lin Cai Jian-Zhong Zhang Xu Yao Jun Gu Quan-Zhong Liu min Zheng Shi-Fa Zhang Jin-Hua Xu Cheng-Xin Li Hao Cheng Qing Guo Wei-Li Pan Shen-Qiu Li Ruo-Yu Li Zai-Pei Guo Zhi-Qi Song Shan-Shan Li Xiu-Qin Dong linda Wang Rong Fu Pascaline Regnault Pascal Charef Ratal Mazur Manmath Patekar Department of Dermatology.Peking University People's Hospital Beijing 100044China Institute of Dermatology Chinese Academy of Medical Science&Peking Union Medical CollegeBeijing 100730China Department of Dermatology Changhai HospitalShanghai 200433China Department of Dermatology Tianjin Medical University General HospitalTianjin 300052China Department of Dermatology Second Affiliated Hospital School of MedicineZhejiang UniversityHangzhouZhejiang 311215China Department of Dermatology General Hospital of Northern Theater CommandShenyangLiaoning 110016China Department of Dermatology Huashan HospitalFudan UniversityShanghai 200040China Department of Dermatology and Venereology Chinese People's Liberation Army General HospitalBeijing 100853China Department of Dermatology Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhouZhejiang 310016China Department of Dermatology Sun Yat-Sen Memorial HospitalGuangzhouGuangdong 510120China Department of Dermatology Zhejiang Provincial People's HospitalZhejiangHangzhouZhejiang 310014China Department of Dermatology Tongji HospitalTongji Medical College of Huazhong University of Science&TechnologyWuhanHubei 430030China Department of Dermatology Peking University First HospitalBeijing 100034China Department of Dermatology West China HospitalSichuan UniversityChengduSichuan 610041China Department of Dermatology First Affiliated Hospital of Dalian Medical UniversityDalianLiaoning 116011China Department of Dermatology The First Hospital of Jilin UniversityChangchunJilin 130021China Department of Dermatology Guangdong General HospitalGuangzhouGuangdong 510080China Beijing Novartis Pharma Co.Ltd. Shanghai 201203China Novartis Pharma AG Basel 6463Switzerland

Background:Psoriasis is a chronic inflammatory skin disease,affecting about 0.6%of the Chinese *** patients are not well controlled by conventional treatments,thus there is need for new treatment *** this study,we assessed the efficacy and safety of secukinumab in Chinese patients with moderate to severe plaque ***:This study was a 52-week,multicentre,randomized,double-blind,placebo-controlled,parallel-group,Phase 3 trial.A sub-population of study participants(≥18 years)of Chinese ethnicity were randomized to receive subcutaneous injections of 300 or 150 mg secukinumab,or *** co-primary endpoints were psoriasis area severity index(PASI)75 and Investigator’s Global Assessment(IGA)0/1 at Week ***:A total of 441 Chinese patients were enrolled in this ***-primary outcomes were achieved;300 and 150 mg secukinumab were superior to placebo as shown in the proportion of patients that achieved PASI 75(97.7%and 87.2%vs.3.7%,respectively;P<0.001),and IGA 0/1(82.3%and 69.7%vs.2.7%;P<0.001)at Week *** efficacy was maintained until Week *** was no increase in overall adverse events with secukinumab relative to placebo throughout the 52-week ***:Secukinumab is highly effective and well tolerated in Chinese patients with moderate to severe plaque psoriasis.

关键词： Chinese Psoriasis IL-17 Dermatology Clinical trial PASI

在初治和经治丙型肝炎病毒基因1b型慢性感染的代偿期肝硬化亚洲成年患者中评价奥比帕利和达塞布韦联合利巴韦林治疗的有效性和安全性

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中华肝脏病杂志 2018年第5期26卷 353-358页

作者：魏来王贵强 Sarah Kopecky-Bromberg 成军诩青汪茂荣许敏段钟平侯金林张明香张跃新唐红赵伟蔺淑梅贾战生牛俊奇高志良袁宏林明华周新民 Luo Yan linda Fredrick Niloufar Mobashery Wang Ye 贾继东北京大学人民医院 100044 北京大学第一医院 100034 AbbVie Inc. North Chicago IL USA60064 首都医科大学附属北京地坛医院北京100015 上海交通大学医学院附属瑞金医院 200025 解放军第八一医院南京210002 广州市第八人民医院 510000 首都医科大学附属佑安医院北京100069 南方医科大学南方医院广州510515 沈阳市第六人民医院 110006 新疆医科大学第一附属医院乌鲁木齐830011 四川大学华西医院成都610041 南京市第二医院 210028 西安交通大学第一附属医院 710065 吉林大学第一医院长春130021 中山大学附属第三医院广州510630 兰州大学第一医院 730000 福州市传染病医院 350001 第四军医大学第一附属医院西安710032 AbbVie.China. Shanghai 100004 首都医科大学附属北京友谊医院北京100050

目的评估奥比帕利（奥比他韦/帕立瑞韦/利托那韦）25/150/100mg,1次/d和达塞布韦（DSV） 250 mg,2次/d联合利巴韦林在丙型肝炎病毒基因1b型感染的代偿期肝硬化中国大陆成年患者中的有效性和安全性. 方法采用开放标签、多中心Ⅲ期临床... 详细信息

目的评估奥比帕利（奥比他韦/帕立瑞韦/利托那韦）25/150/100mg,1次/d和达塞布韦（DSV） 250 mg,2次/d联合利巴韦林在丙型肝炎病毒基因1b型感染的代偿期肝硬化中国大陆成年患者中的有效性和安全性. 方法采用开放标签、多中心Ⅲ期临床试验,在中国大陆、中国台湾地区和韩国开展,纳入初治和经治基因1b型丙型肝炎病毒感染的代偿期肝硬化（Metavir 评分纤维化分期=F4）成年患者,接受奥比他韦/帕立瑞韦/利托那韦和达塞布韦联合利巴韦林治疗12周.评估患者停药12周获得的持续病毒学应答（SVR）及24周获得的SVR率,并在接受至少1次研究药物的患者中评估有效性和安全性. 结果共纳入63例中国大陆患者,其中62例（98.4％）患者基线Child-Pugh评分为5分.患者总体SVR12率及SVR24率为100％（95％ CI：94.3％～ 100.0％）.发生的大多数不良事件为轻度.常见（发生率≥10％）所有级别不良事件和实验室异常包括总胆红素升高（36.5％）、乏力（19.0％）、非结合胆红素升高（19.0％）、结合胆红素升高（17.5％）和贫血（14.3％）.3例（4.8％）患者发生≥3级不良事件,均被研究者判定为与研究药物无关.无患者出现导致提前停药的不良事件. 结论丙型肝炎病毒基因1b型感染的代偿期肝硬化中国大陆患者接受奥比他韦/帕立瑞韦/利托那韦和达塞布韦联合利巴韦林治疗12周,停药12周和24周的SVR均为100％.耐受性及安全性良好,大多数不良事件为轻度.

关键词：肝炎,丙型,慢性肝硬化直接抗病毒药物