PHB1 inhibits the proliferation of nasopharyngeal carcinoma cells via inactivation of the NF-κB signaling pathway
作者单位：Key Laboratory of Translational Radiation OncologyHunan ProvinceHunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of MedicineCentral South University
会议名称：《2017年中国肿瘤标志物学术大会暨第十一届肿瘤标志物青年科学家论坛》
会议日期：2017年
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
关 键 词：Nasopharyngeal carcinoma PHB1 NF-κB cell proliferation signaling pathway
摘 要：Objective: PHB1 has been proved tumor-associated and functions as tumor suppressor in multiple cancer cells. This study aimed to investigate the effects of PHB1 on the proliferation in human nasopharyngeal carcinoma(NPC) cell lines and its mechanisms. Method: PHB1 was stably overexpressed in CNE1 and HNE1 cells. Cell proliferation was assessed by colony formation and CCK8 assay. Cell-cycle distribution and apoptosis were analyzed using flow cytometry. Luciferase reporter assays, immunofluorescent assay, western blot and quantitative RT-PCR were used to detect the NF-κB activity and downstream signaling pathway expression. Results: PHB1 significantly inhibited the proliferation of NPC cell lines CNE1 and HNE1 in vitro. PHB1 induced an apparent cell cycle arrest at the G1/S phase and apoptosis, which was accompanied by an obvious inactivation of the NF-κB signaling pathway. PHB1 led to a remarkable decrease the expression of NF-κB p65, IKKβ, cyclin D1, cyclin-dependent kinase(CDK) 4 and Bcl-2, obviously increased the expression of IκBα, p21, Bax at both mRNA and protein levels in NPC cells. In addition, PHB1 restrained the LPS-induced activation of NF-κB signaling via inhibiting p65 translocation into nuclear. Conclusion: PHB1 could inhibit cell proliferation in vitro via inactivation of the NF-κB signaling pathway. PHB1 maybe serve as a tumor suppressor gene and a potential therapeutic target against NPC.