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Optimal culture conditions for the generation of natural killer cell-induced dendritic cells for cancer immunotherapy

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Cellular & Molecular Immunology 2012年第1期9卷 45-53页

作者： Thanh-Nhan Nguyen-Pham Deok-Hwan Yang Truc-Anh Thi Nguyen Mi-Seon Lim Cheol Yi Hong Mi-Hyun kim Hyun Ju Lee Youn-Kyung Lee Duck Cho Soo-Young Bae Jae-Sook Ahn Yeo-kyeoung kim Ik-Joo Chung Hyeoung-Joon kim Je-Jung Lee Research Center for Cancer Immunotherapy Chonnam National University Hwasun HospitalJeollanamdoKorea Department of Hematology-Oncology Chonnam National University Hwasun HospitalJeollanamdoKorea Vaxcell-Bio Therapeutics JeollanamdoKorea The Brain Korea 21 Project Center for Biomedical Human Resources at Chonnam National UniversityGwangjuKorea

Dendritic cell(DC)-based vaccines continue to be considered an attractive tool for cancer *** require an additional signal from the environment or other immune cells to polarize the development of immune responses toward T helper 1(Th1)or Th2 *** play a role in natural killer(NK)cell activation,and NK cells are also able to activate and induce the maturation of *** investigated the types of NK cells that can induce the maturation and enhanced function of DCs and the conditions under which these interactions *** that were activated by resting NK cells in the presence of inflammatory cytokines exhibited increased expression of several costimulatory molecules and an enhanced ability to produce *** cell-stimulated DCs potently induced Th1 polarization and exhibited the ability to generate tumor antigen-specific cytotoxic T lymphocyte *** data demonstrate that functional DCs can be generated by coculturing immature DCs with freshly isolated resting NK cells in the presence of Toll-like receptor agonists and proinflammatory cytokines and that the resulting DCs effectively present antigens to induce tumor-specific T-cell responses,which suggests that these cells may be useful for cancer immunotherapy.

关键词： cancer immunotherapy dendritic cells natural killer cells signal

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Type I and II interferons enhance dendritic cell maturation and migration capacity by regulating CD38 and CD74 that have synergistic effects with TLR agonists

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Cellular & Molecular Immunology 2011年第4期8卷 341-347页

作者： Thanh-Nhan Nguyen-Pham Mi-Seon Lim Truc Anh Thi Nguyen Youn-Kyung Lee Chun-Ji Jin Hyun Ju Lee Cheol Yi Hong Jae-Sook Ahn Deok-Hwan Yang Yeo-kyeoung kim Ik-Joo Chung Byoung Chul Park Hyeoung-Joon kim Je-Jung Lee Research Center for Cancer Immunotherapy Chonnam National University Hwasun HospitalJeollanamdoKorea Department of Hematology-Oncology Chonnam National UniversityGwangjuKorea The Brain Korea 21 Project Center for Biomedical Human Resources at Chonnam National UniversityGwangjuKorea Vaxcell-Bio Therapeutics JeollanamdoKorea Korea Research Institute of Bioscience and Biotechnology(KRIBB) DaejeonKorea

The major limitation for the maturation of dendritic cells(DCs)using Toll-like receptor(TLR)agonists is their decreased ability to migrate into lymph nodes compared with conventional ***38 can be used as a multifunctional marker to modulate migration,survival and Th1 responses of ***74 has been shown to negatively regulate DC *** goal of this study was to investigate the combinations of TLR agonists and interferons(IFNs)that most effectively regulate CD38 and CD74 expression on *** TLR agonist stimulation in combination with IFN-a and IFN-c was the best method for regulating CD38 and CD74 expression and inducing the highest secretion of *** in vitro migration assay showed that DCs treated with this combination had significantly enhanced migratory ability,similar to that observed in cells expressing CD38,CD74 and *** results of this study suggest that an alternative maturation protocol in which two TLR ligands are combined with type I and II IFNs generates potent DCs that have both a high migratory capacity and high IL-12p70 production.

关键词： CD38 CD74 dendritic cells interferon Toll-like receptor

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股骨头坏死塌陷的预测：改良Kerboul法在MRI上的应用

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骨科动态 2007年第1期3卷 9-14页

作者： YONG-CHAN HA WOON HWAJUNG JANG-R.ak kim Nak HOON SEONG SHIN-YOON kim KYUNG-HOI KOO 陈云苏（译）不详上海第六人民医院骨科 200233

背景：在MRI上测量相加坏死角度可预测股骨头坏死致髋关节塌陷的危险性。方法：对连续33例37髋患者的早期骨坏死的髋关节进行研究。采用改良Kerboul等方法，测量正中冠状位和正中矢状位MRI上（非前后位和侧位X线片）骨坏死累及股骨头表... 详细信息

背景：在MRI上测量相加坏死角度可预测股骨头坏死致髋关节塌陷的危险性。方法：对连续33例37髋患者的早期骨坏死的髋关节进行研究。采用改良Kerboul等方法，测量正中冠状位和正中矢状位MRI上（非前后位和侧位X线片）骨坏死累及股骨头表面的弧度，计算两角度之和。基于计算的相加坏死角度的度数，将髋关节分为四组：1级（〈200°），2级（200°-249°），3级（250°-299°），4级（≥300°在初始评估后，患者被随机分入髓芯减压组或非手术组进行治疗，同时接受正规随访直至股骨头出现塌陷或至少观察5年。结果：7例4级的髋关节和16例3级的髋关节在36个月出现了股骨头塌陷。9例2级的髋关节中的6例出现塌陷，5例1级的髋关节均未出现塌陷（10g-rank检验，p〈0．01）。研究期间，4例相加坏死角度≤190。（低风险组）的髋关节无1例出现塌陷，25例相加坏死角度≥240°（高风险组）的髋关节均出现塌陷，8例相加坏死角度界于190°-240°（中风险组）的髋关节中有4例（50％）出现塌陷。结论：使用MRI扫描代替X线片测量Kerboul相加坏死角度评估股骨头坏死致髋关节塌陷的可能性是一种好的方法。可信水平：预后性研究，上级。进一步可信度参见作者介绍。

关键词：股骨头坏死塌陷 MRI扫描改良预测 X线片测量早期骨坏死髋关节关节塌陷

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Comparison of anterior segment optical coherence tomography findings in acanthamoeba keratitis and herpetic epithelial keratitis

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International Journal of Ophthalmology(English edition) 2018年第8期11卷 1416-1420页

作者： Young Min Park Jong Soo Lee Ji-Myong Yoo Jong Moon Park Seong-Wook Seo In-Young Chun Seong Jae kim Department of Ophthalmology School of Medicine Gyeongsang National University Jinju 52727 South Korea Gyeongsang National University Changwon Hospital Samjeongja-ro Seongsan-gu Changwon-si Gyeongsangnamdo 51472 South Korea Department of Ophthalmology School of Medicine Pusan National University and Medical Research Institute Pusan National University Hospital Pusan 49241 South Korea Institute of Health Science Gyeongsang National UniversityJinju 52727 South Korea

This study is to investigate the characteristic features of Acanthamoeba keratitis（ak） that differentiating it from herpetic epithelial keratitis（HEK） using anterior segment optical coherence tomography（AS-OCT）. Medical records of three eyes of each ak and herpetic keratitis who had AS-OCT examination were reviewed in this study. Slitlamp biomicroscopy and AS-OCT was performed on the initial visit and on every follow-up visits in all patients. In all three ak cases, reflective bands in the corneal stroma that correspond to the area of radial keratoneuritis were observed. The depth of the reflective bands varied in each case. After ak treatment, slit-lamp biomicroscopy confirmed that radial keratoneuritis had resolved and AS-OCT confirmed that reflective bands in the corneal stroma had also disappeared in all patients. Unlike the AS-OCT results found in ak, highly reflective HEK lesions were observed only in the subepithelial area, not in the stroma. AS-OCT seems to be helpful analyzing the specific depth of the lesion which enables to distinguish ak from HEK.

关键词： acanthamoeba optical coherence tomography herpes herpetic keratitis

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A highly annotated whole- genome sequence of a Korean individual

A highly annotated whole- genome sequence of a Korean indivi...

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全国生物遗传多样性高峰论坛

作者： Jong-Il kim Joseph Thakuria Jeong-Sun Seo Macrogen Inc. SeoulKorea Genomic Medicine Institute Medical Research CenterSeoul National UniversitySeoulKorea Department of Genetics Harvard Medical SchoolBostonMassachusettsUSA

We provide a highly annotated,whole - genome sequence for a Korean individual,known as *** genome of ak1 was determined by an exacting,combined approach that included whole - genome shotgun sequencing(27.83 coverage),targeted bacterial artificial chromosome sequencing,and high - resolution comparative genomic hybridization using custom microarrays featuring more than

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Comparison of Specific Activity and Cytopathic Effects of Purified 33-Kda Serine Proteinase from Acanthamoeba Strains with Different Degree of Virulence

Comparison of Specific Activity and Cytopathic Effects of Pu...

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12th International Congress of Protozoology

作者： Won-Tae kim Hyun-Hee Kong Young-Ran Ha Yeon-Chul Hong Hae-Jin Jeong Hak-Sun Yu Dong-II Chung Department of Parasitology Kyungpook National University School of Medicine Taegu 700-422 Department of Parasitology Kyungpook National University School of Medicine Taegu 700-422 Department of Parasitology Kyungpook National University School of Medicine Taegu 700-422 Department of Parasitology Kyungpook National University School of Medicine Taegu 700-422 Department of Parasitology Pusan National University School of Medicine Pusan 602-702KOREA Department of Parasitology Pusan National University School of Medicine Pusan 602-702KOREA Department of Parasitology Kyungpook National University School of Medicine Taegu 700-422

＜正＞The pathogenic mechanism of granulomatous amoebic encephalitis （GAE） and amoebic keratitis （ak） by Acanthamoeba has yet to be clarified. Protease has been recognized to play an important role in the pathogenesis of GAE and ak. In the present study, we have compared specific activity and cytopathic effects of purified 33-kDa serine proteinases from Acanthamoeba strains with different degree of virulence (4. healyi OC-3A, A. lugdunensis KA/E2, A. castellanii Neff. Trophozoites of three strains

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Colonoscopic yield of colorectal neoplasia in daily clinical practice

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World Journal of Gastroenterology 2009年第9期15卷 1085-1092页

作者： Jochim S Terhaar sive Droste Mike E Craanen Rene WM van der Hulst Joep F Bartelsman Dick P Bezemer kim R Cappendijk Gerrit A Meijer Linde M Morsink Pleun Snel Hans ARE Tuynman Roy LJ van Wanrooy Eric IC Wesdorp Chris JJ Mulder Department of Gastroenterology and Hepatology VU University Medical CentrePO Box 70571007 MBAmsterdamThe Netherlands Gastroenterology and Hepatology Kennemer Gasthuis2000 AKHaarlemThe Netherlands Gastroenterology and Hepatology Academic Medical Centre1100 DDAmsterdamThe Netherlands Epidemiology and Biostatistics VU University Medical Centre1007 MBAmsterdamThe Netherlands Pathology VU University Medical Centre1007 MBAmsterdamThe Netherlands Gastroenterology and Hepatology Slotervaart Hospital1006 BKAmsterdamThe Netherlands Gastroenterology and Hepatology Medical Centre Alkmaar1800 AMAlkmaarThe Netherlands Gastroenterology and Hepatology Sint Lucas Andreas Hospital1061 AEAmsterdamThe Netherlands

AIM:To assess the prevalence and location of ad-vanced neoplasia in patients undergoing colonoscopy,and to compare the yield per ***:In a multicenter colonoscopy survey (n = 18 hospitals) in the Amsterdam area (Northern Holland),data of all colonoscopies performed during a three month period in 2005 were analyzed. The location and the histological features of all colonic neoplasia were recorded. The prevalence and the distribution ofadvanced colorectal neoplasia and differences in yield between indication clusters were evaluated. Advanced neoplasm was defi ned as adenoma > 10 mm in size,with > 25% villous features or with high-grade dyspla-sia or ***:A total of 4623 eligible patients underwent a total colonoscopy. The prevalence of advanced neo-plasia was 13%,with 281 (6%) adenocarcinomas and 342 (7%) advanced adenomas. Sixty-seven percent and 33% of advanced neoplasia were located in the distal and proximal colon,respectively. Of all patients with right-sided advanced neoplasia (n = 228),51% had a normal distal colon,whereas 27% had a syn-chronous distal adenoma. Ten percent of all colono-scopies were performed in asymptomatic patients,7% of whom had advanced neoplasia. In the respective procedure indication clusters,the prevalence of right-sided advanced neoplasia ranged from 11%-57%. CONCLUSION:One out of every 7-8 colonoscopies yielded an advanced colorectal neoplasm. Colonoscopy is warranted for the evaluation of both symptomatic and asymptomatic patients.

关键词： Colorectal cancer Screening Advanced neoplasia Colonoscopy Adenoma

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在相同压力环境下反应不同的HEK293和HepG2细胞中腺苷酸激酶4表达不同

在相同压力环境下反应不同的HEK293和HepG2细胞中腺苷酸激酶4表达...

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中国畜牧兽医学会2013年学术年会

作者：孔凡志 Bert Binas Jae Hee Moon Sung Soo Kang Hyo Joon kim 黑龙江八一农垦大学动物科技学院韩国汉阳大学安山校区分子生命科学部干细胞研究室韩国汉阳大学安山校区分子生命科学部分子免疫学研究室

目的腺苷酸激酶4(ak4)属于单核苷酸激酶家族成员参与能量代谢。近期有报道称ak4在应激反应中起到保护作用:当HEK293处于低氧压力下时ak4表达量上升,细胞的增殖和活力未受影响,然而,使用RANi封闭ak4的表达后,抑制了细胞的增值促进了细胞... 详细信息

目的腺苷酸激酶4(ak4)属于单核苷酸激酶家族成员参与能量代谢。近期有报道称ak4在应激反应中起到保护作用:当HEK293处于低氧压力下时ak4表达量上升,细胞的增殖和活力未受影响,然而,使用RANi封闭ak4的表达后,抑制了细胞的增值促进了细胞死亡。为了进一步证明ak4与细胞增殖之间的关系我们首次在HepG2细胞中研究了ak4的表达及其生理功能。材料方法HEK293细胞;HepG2细胞;RNAi;Microarray等。结果试验研究发现ak4在HepG2细胞中的表达量显著高于HEK293,并且当HepG2细胞处于低氧压力下时伴随ak4表达量下降,明显抑制了细胞增殖和促进了细胞死亡。然而通过RNAi封闭ak4的表达后两种细胞的增值均受到抑制,并且促进细胞死亡,虽然只有HepG2细胞的细胞周期受到了影响。所以抑制ak4的表达总是与细胞死亡相关。讨论以上结果更加深入的证明了ak4在新的生理压力下的保护功能。我们的结果也支持这样的假设:不同的本底ak4表达量反应了细胞处于的不同基础压力水平,导致了细胞在额外压力下导致了不同的反应。

关键词： HepG2细胞 HEK293细胞低氧压力 RNAi

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不能忘却的纪念：—记DFI ak75—AL“黄金纪念版”主机板

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家庭电脑世界 2001年第7期 8-8页

作者： kim

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